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Synthesis and characterization of iron oxide/functionalized graphene oxide nanocomposites for highly efficient DNA isolation

Akrami, M ; Sharif University of Technology | 2023

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  1. Type of Document: Article
  2. DOI: 10.1016/j.mseb.2023.116401
  3. Publisher: Elsevier Ltd , 2023
  4. Abstract:
  5. In this research, Fe3O4 nanoparticles/graphene oxide (Fe3O4/GO) and Fe3O4/polypyrrole/graphene oxide (Fe3O4/PPy/GO) magnetic nanocomposites were fabricated for DNA isolation. The prepared aminated nanocomposite conducted perfect DNA isolation characteristics. The results showed that the Freundlich isotherm and the pseudo-second-order models well-matched the equilibrium extraction data and kinetic outcomes, respectively. The electrophoresis findings confirmed the efficacy of as-prepared nanocomposites for DNA adsorption/desorption. In addition, the adsorption capacity was reduced by 8.9% after five cycles, implying that Fe3O4/PPy/GO nanocomposite can maintain increased isolation ability after these cycles. The result of the MTT assay revealed that Fe3O4/PPy/GO nanocomposites were not toxic and had high biocompatibility. Thus, the prepared Fe3O4/PPy/GO nanocomposites would be a beneficial device for DNA isolation in biological uses. © 2023 Elsevier B.V
  6. Keywords:
  7. DNA isolation ; Fe3O4 ; Graphene oxide ; Magnetic nanoparticle ; Nanocomposites
  8. Source: Materials Science and Engineering: B ; Volume 292 , 2023 ; 09215107 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0921510723001435