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Effect of calcium peroxide particles as oxygen-releasing materials on cell growth and mechanical properties of scaffolds for tissue engineering

Mollajavadi, M.Y ; Sharif University of Technology | 2023

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  1. Type of Document: Article
  2. DOI: 10.1007/s13726-023-01147-y
  3. Publisher: Springer Science and Business Media Deutschland GmbH , 2023
  4. Abstract:
  5. One of the challenges of building complex and functional tissues and organs in tissue engineering is transporting nutrients and oxygen to the tissue. The use of oxygen-releasing materials is one solution to overcome this challenge. In this study, alginate (Alg) 4% (w/v) was used as the main material for the scaffold, and calcium peroxide (CPO) was used to release oxygen. CPO can react with water and finally release oxygen, which leads to the elimination of hypoxia and effective distribution of oxygen in the scaffold. Multiple tests, including the oxygen release profile, degradability, swelling, porosity, and SEM images, performed on the bulk of the material showed that increasing the percentage of CPO in the scaffold led to an increase in oxygen release, porosity, and degradation. All CPO-containing samples could maintain oxygen concentration in solution above 5 mg/L for 1 week. Increasing CPO concentration resulted in an increase in scaffold porosity from about 48% in the control sample (Alg100-CPO0), which contains no CPO, to about 83% in the alginate hydrogel with CPO:Alg (% by w/w) equal to 5% (Alg100-CPO5). Additionally, Young’s modulus of the two samples, Alg100-CPO1 [CPO:Alg (% by w/w) equal to 1%] and Alg100-CPO5, was about 58 ± 3 and 74 ± 5 kPa, respectively. Alg100-CPO5 showed low cell viability compared to other samples. Finally, Alg100-CPO1 was chosen as the optimum sample with excellent cell viability, oxygen release, and mechanical properties of the scaffold for further studies. Graphical abstract: [Figure not available: see fulltext.] © 2023, Iran Polymer and Petrochemical Institute
  6. Keywords:
  7. Alginate ; Calcium peroxide ; Hypoxia ; Oxygen-releasing materials ; Tissue engineering
  8. Source: Iranian Polymer Journal (English Edition) ; Volume 32, Issue 5 , 2023 , Pages 599-608 ; 10261265 (ISSN)
  9. URL: https://link.springer.com/article/10.1007/s13726-023-01147-y