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A new slopping micro-channel Dielectrophoresis-Based System for Non-invasive Same-Size Cells Separation

Alipour, Z ; Sharif University of Technology | 2023

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  1. Type of Document: Article
  2. DOI: 10.1109/ICBME61513.2023.10488594
  3. Publisher: Institute of Electrical and Electronics Engineers Inc , 2023
  4. Abstract:
  5. In this paper, we proposed a continuous label-free microfluidic chip design for same-size cell separation. The design is based on dielectrophoresis (DEP) force, a rapid and semiautomated method incorporating the difference in dielectric properties of cells as the basis of the separation. This device employs four pairs of planar electrodes, where the cells under positive DEP (PDEP) phenomenon move away from the central streamline to the bottom outlet, while cells experiencing negative DEP (NDEP) force do vice versa. COMSOL Multiphysics is used to optimize channel geometry and electrode design. Preserving the viability of cells while moving toward their respective outlets, the microchannel is designed in a new slopping manner to decrease the required electric field. The maximum applied electric field of 3.64 imes 10{5} V/m (i.e. voltage amplitude of 10v) is lower than similar reported works with planar electrodes. Separation efficacy is tested by separating dead and live yeast at 1 MHz, where dead yeasts experience NDEP force and live yeasts experience PDEP. We utilized an accessible and easy fabrication process. Electrodes are made of gold using a standard lithography process, and microchannel is created by soft lithography using 3d printed mold and PDMS, an accessible and biocompatible material. The flow rates of the main inlet and sheathes are 0.015 muL/min and 0.072 muL/min, respectively, employing accurate homemade injection pumps. Both simulations and experimental results confirm the successful performance of the microfluidic chip in this design. © 2023 IEEE
  6. Keywords:
  7. 2D electrodes ; Bio-particle separation ; Dielectrophoresis ; Microchannel design ; Microfluidics
  8. Source: 2023 30th National and 8th International Iranian Conference on Biomedical Engineering, ICBME 2023 ; 2023 , Pages 110-114 ; 979-835035973-2 (ISBN)
  9. URL: https://ieeexplore.ieee.org/document/10488594