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Development of a Colorimetric Sensor Based on the Anti-Etching of Gold Nanorods for Detection and Discrimination of Neurotransmitters
Daneshmandi, Mahsa | 2024
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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 57085 (03)
- University: Sharif University of Technology
- Department: Chemistry
- Advisor(s): Hormozinezhad, Mohammad Reza
- Abstract:
- Neurotransmitters can be classified depending on their molecular structure, such as monoamines, which include dopamine (DA), epinephrine (EP), norepinephrine (NE), and levodopa (L-dopa), which acts as a metabolic precursor for dopamine. Simultaneous identification and measurement of these neurotransmitters in human bodily fluids is crucial for the early identification of various disorders. Our current study relies on gold nanorods as a colorimetric sensor element for the identification and classification of biological neurotransmitters. Initially, the gold nanorods undergo corrosion and annihilation in neutral conditions by means of a corrosive substance. Subsequently, in the presence of neurotransmitters, the anti-corrosion mechanism of the gold nanorods is influenced by the varying degrees of oxidation of the analytes triggered by NBS, thereby impeding the process. The reaction of AuNRs with NBS results in the formation of a distinct pattern resembling a fingerprint, accompanied by the generation of unique colors. These colors serve as an instrument to distinguish and measure the examined analytes. The proposed sensor was assessed using multivariate analysis techniques, such as linear discriminant analysis and partial least squares regression. The linear range achieved for the epinephrine, norepinephrine, levodopa, and dopamine, were (25-67), (1.93-25), (4.04-25), and (1.84-60) micromolar, respectively. The sensor is capable of measuring very small quantities of neurotransmitters, with detection limits of 0.62, 0.47, 0.49, and 0.92 micromolar for epinephrine, norepinephrine, levodopa, and dopamine analytes, respectively. The statistical data obtained at this point also demonstrated the potential capability of the proposed sensor in discerning and differentiating between single-component and multi-component solutions of the aforementioned neurotransmitters. Moreover, the researched sensor effectively detects and quantifies neurotransmitters in real samples, including urine
- Keywords:
- Gold Nanorods ; Anticorrosive Coating ; Colorimetric Sensor ; Neuro Transmitter ; Core-Shell Structure
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