Sharif Digital Repository

Flagella is the organelle involved in motility of Salmonella and plays an important role in colonization of Salmonella. Flagellins is the structural subunits of flagellar filament that is known as an adjuvant. Recombinant production of flagellin of S. enteritidis is advantageous due to the removal of pathogenic microorganism from the production process and improved process safety. E.coli is an appropriate host for the expression of recombinant proteins. However, its major obstacle is the formation of inclusion bodies. Inclusion bodies are aggregated inactive proteins and the recovery of proteins in the active form from these aggregates usually has a low yield. Therefore, the objective of our... 

Lipases (Triacyl Glycerol Acyl Hydrolase, EC.3.1.1.3) are multidimensional biocatalysts which have many applications in food industrials, dairy, fats and oils processing, detergents, synthesis of pure chemical and pharmaceutical materials, paper industrial and cosmetics. Generally, industrial enzymes are produced extracellularly, so their separation from a low-cost complex medium (usually used for production) is one of the cost stages in their production process. Filtration is the most economical method of separation in the production of enzymes, but due to the small size of colloidal particles and cell masses present in the fermentation environment, its use is subject to limitations. In... 

Heat pipes are useful devices in heat transfer, renewable energies and cooling systems in particular. According to various application of this systems in different industries, performance improvement of this devices has gained much importance. Working fluid is one of the effective factors on pulsating heat pipes. Since heat transfer in PHPs is natural convection and two-phase, specific heat and thermal conductivity of working fluid have an important role in the performance of pulsating heat pipes. In the present study, the effect of using nano-encapsulated PCM along with reduced graphene oxid (RGO) dispersed in water and their mixture as working fluid has on the performance of PHPs has been... 

In this research, direct synthesis of dimethyl ether (DME) from CO2 hydrogenation in three phase slurry reactor was investigated. Bifunctional catalysts composed of Cu- ZnO-Al2O3-ZrO2 as hydrogenation component and zeolite Na-Mordenite as dehydration component, with different Al2O3 and ZrO2 contents were synthesized by coprecipitating sedimentation and influences of Al2O3 and ZrO2 on CO2 conversion and DME selectivity were evaluated. Results show, both Al2O3 and ZrO2 enhanced conversion, but Al2O3 markedly increase selectivity of DME. However, when the total contents of Al2O3 and ZrO2 was increased up to 16 wt% , CO2 conversion and DME selectivity remarkably decreased. BET and XRD analyzes... 

In this study, a mixture of nano-encapsulated phase change materials in water has been used as a working fluid in a pulsating heat pipe in order to investigate its thermal performance. The results of the experiments showed that using the nano-encapsulated paraffin dispersed in water as working fluid improves heat transfer and decreases the thermal resistance of the pulsating heat pipe. On the other hand, there is an optimal concentration among tested concentrations for nano-encapsulated paraffin, in which increasing its concentration due to the increased dynamic viscosity of the fluid causes an increase in the thermal resistance of the pulsating heat pipe. Enhancement in the thermal...