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Spectrophotometric Determination of Glutathione and Cysteine Based on Aggregation of Colloidal Gold Nanoparticles
, M.Sc. Thesis Sharif University of Technology ; Hormozi Nezhad, Mohammad Reza (Supervisor)
Abstract
low-molecular-weigth aminothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play a critical role in many biochemical pathways . Their level in biological fluids such as human plasma and urine are important for clinical diagnostics of a variety of diseases.
We report herein the development of a highly sensitive colorimetric method for detection of cysteine and glutathione using the citrate capped gold nanoparticles (Au-NPs). This thiol containing molecules exhibit intriguing reactivities with Au nanoparticles. The reactivity involves the displacement of the citrate shell by the thiolate shell which is followed by intermolecular electrostatic interactions or...
We report herein the development of a highly sensitive colorimetric method for detection of cysteine and glutathione using the citrate capped gold nanoparticles (Au-NPs). This thiol containing molecules exhibit intriguing reactivities with Au nanoparticles. The reactivity involves the displacement of the citrate shell by the thiolate shell which is followed by intermolecular electrostatic interactions or...
Simultaneous Determination of Glutathione- Cysteine, and Detection of Mercury Ion (II) Based on Fluorescence Resonance Energy Transfer between Gold Nanoparticles and Fluorescein Isothiocyanate
, M.Sc. Thesis Sharif University of Technology ; Hormozi-Nezhad, Mohammad Reza (Supervisor)
Abstract
Turn-On Fluorescence detection is a selective and sensitive method for many analytical purposes. Fluorescence quenching is a commonly observed consequence when fluorophores and quencher, gold nanoparticles (Au NPs) in this work, are in same media. Here, we report a “turn-on” fluorescent nanoprobe for simultaneous determination of glutathione and cysteine using H-point standard addition method (HPSAM). This probe develops based on the fluorescence resonance energy transfer (FRET) between Au NPs and fluorescein isothiocyanate (FITC), in which FITC acts as the donor and Au NPs as the acceptor. FRET causes the fluorescence intensity of FITC strictly quenched as a result of noncovalently adsorbed on...
Design of Nature-Inspired Multicolor Sensor for Detection and Discrimination of Biothiols based on Anti-Etching on Gold Nanorods
, M.Sc. Thesis Sharif University of Technology ; Hormozinezhad, Mohammad Reza (Supervisor)
Abstract
Biological thiols are crucial substances that play an essential role in several biological processes and activities in the body. Hence, the fluctuation of their concentrations can be used as a diagnostic sign for numerous disorders. In contrast, the ratio of these thiols to their disulfide form within the cell is useful as an indicator for determining cytotoxicity. Hence, it is crucial for develop a simple yet efficient analytical technique for quickly recognizing and determining them. The purpose of this study is to develop a plasmonic colorimetric sensor by utilizing gold nanorods (AuNRs). This sensor aims to accurately measure and distinguish between cysteine (CYS) and glutathione (GSH),...