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Design, Simulation, and Fabrication of DEP-Based Microfluidic Chip for Bioparticles Separation
, M.Sc. Thesis Sharif University of Technology ; Fardmanesh, Mehdi (Supervisor)
Abstract
Microfluidics, employing fluids in structures with micrometer dimensions, is an emerging technology that has brought great opportunities to develop various devices including lab-on-a-chip systems. Lab-on-a-chip systems have the advantages of integrating several stages of different analytical methods, application in diverse fields, consumption of small volumes of samples, and portability. One of the crucial applications of lab-on-a-chip devices is sorting cells and biological particles, done by different methods. In separator devices, high separation efficiency and repeatability are critical. When a neutral particle is polarized in a non-uniform electric field, the dielectrophoresis force...
Design and Fabrication of a Microfluidic System Based on Dielectrophoresis for Enrichment and Separation of Bioparticles
, M.Sc. Thesis Sharif University of Technology ; Mohammadi, Ali Asghar (Supervisor)
Abstract
The main purposes of this project are simulation, design, and fabrication of a microfluidic system based on dielectrophoresis (DEP) exploiting AC voltages for separation of microparticles. Generally, this project can be divided into two parts. First, the engineering section including design and fabrication of microchip, and second biological samples preparation for investigating the system performance. The main advantages of proposed system are eliminating joule heating, a significant problem in insulating based dielectrophoresis systems, overcoming problems related to metal contamination in biological samples, the main problem of conventional dielectrophoresis platforms, and removing...
Separation and Microfluidic Analysis of one Cancer Biomarker (Exosome) by Means of Microfluidic Chip Microfluidc Analysis Exosome Toward Liquid Biopsy for Cancer Tumor Cell
, M.Sc. Thesis Sharif University of Technology ; Saidi, Mohammad Said (Supervisor) ; Firouzabadi, Bahar (Co-Supervisor)
Abstract
The recent breakthroughs in the lab-on-a-chip technology will not be directly used as clinical devices unless the specific applications are carefully tested and certified. As there is a growing trend towards microfluidic devices for biomedical research, an active microfluidic device was developed. Common microfabrication methods were used to develop a microchip to separate and concentrate exosome and particular cell based on the dielectrophoresis phenomenon. Unique capabilities of the dielectrophoresis phenomenon make it a powerful tool for micro particle manipulation in micro scale. In this research, geometry for the mentioned purpose is designed and optimized. Three-dimensional...
Design and Fabrication of a Microfluidic Chip for Live and Dead Cell Separation
, M.Sc. Thesis Sharif University of Technology ; Firouzbakhsh, Keykhosrow (Supervisor) ; Saeedi, Mohammad Saeed (Supervisor)
Abstract
The recent breakthroughs in the lab-on-a-chip technology will not be directly used as clinical devices unless the specific applications are carefully tested and certified. As there is a growing trend towards microfluidic devices for biomedical research, an active microfluidic device was developed. Common microfabrication methods were used to develop a microchip to separate and concentrate live and dead cells based on the dielectrophoresis phenomenon. Unique capabilities of the dielectrophoresis phenomenon make it a powerful tool for micro particle manipulation in micro scale. In this research, geometry for the mentioned purpose is designed and optimized. Three-dimensional microelectrodes are...
Design and Numerical Simulation of a Micro-chip for Separating CTCs from Peripheral Blood
, M.Sc. Thesis Sharif University of Technology ; Saeedi, Mohammad Saeed (Supervisor)
Abstract
According to studies, cancer is the second leading cause of death in the world and millions of people die from this disease every year. Early diagnosis is one of the most important factors in controlling and treating the disease. The ability of circulating tumor cells to diagnose and monitor the disease has led many researchers to focus on studying and researching these cells in recent years. However, due to the fact that the concentration of this type of cells in the blood is very low, their isolation is associated with challenges. Various methods isolate CTCs based on physical, biological and other differences in characteristics. In this study, we investigate performance of a microfluidic...
CTC Cluster Separation on Microfluidics Chip
, M.Sc. Thesis Sharif University of Technology ; Firouzabadi, Bahar (Supervisor) ; Saidi, Mohammad Said (Supervisor)
Abstract
According to statistics in 2012, around 14 million people worldwide have cancer, and 8 million have died that year, according to estimates, this number will increase to 21 millions new cancer case and 13 million mortality in 2030. Despite years of research on cancer, many questions about the mechanism of the spread of cancer in the body remain unanswered so far. The proposed models identify that the cells isolated from the primary tumor and transmit them to the circulatory system in the form of circulating tumor cells (CTCs) or in the form of clusters as responsible for the incidence of cancer. This process is called metastasis. Recent studies have reported the high potential of metastasis...
Cell Separation via Nano-porous Molecularly Imprinted Polymer
, M.Sc. Thesis Sharif University of Technology ; Abdekhodaie, Mohammad Jafar (Supervisor) ; Nematollah Zade, Ali (Co-Advisor)
Abstract
Molecularly imprinted polymers (MIPs) with synthetic recognition sites are capable of detecting and separating chemical species in complex environments. These characteristics are of increasing interest for bioanalytical applications. The chief advantages of MIPs are mechanical and chemical stability, low cost, and ease of preparation. Recently, imprinting technology has been used for fabrication polymer thin films with recognition sites of proteins, plant viruses, and yeasts. Also few investigations applied lithography for synthesis of cell imprinted polymers targeting cell detection and observation of cell morphology.
For the first time, we demonstrate surface imprinting for the...
For the first time, we demonstrate surface imprinting for the...
Puzzle of Jobless Growth and Growthless Employment: Evidences from Iranian Labor Market
, M.Sc. Thesis Sharif University of Technology ; Nili, Masoud (Supervisor) ; Joshaghani, Hossein (Supervisor)
Abstract
From 1384 to 1395, the labor market in Iran has experienced a mysterious behavior in total number of employment, which we call it puzzle of jobless growth and growthless employment. By studying only the net changes in total number of employment, some important information neglected in explaining this phenomenon. To this end, it is common in labor economics to use dynamic labor market indicators such as job flows and labor flows. In this research, we first calculate labor flows from 1384 to 1395 and job flows from 1382 to 1392 by economic sector, firm size, job classification, occupational insurance coverage, gender and job type. Then the following question will be answered: Is the...
Treatment of Triclosan Through Enhanced Microbial Biodegradation
, M.Sc. Thesis Sharif University of Technology ; Yaghmaei, Soheila (Supervisor) ; Ghobadi Nejad, Zahra (Supervisor)
Abstract
Triclosan is widely used as an antibacterial agent in personal health care products, especially in recent years with the spread of the Coronavirus. This substance is one of the emerging contaminants, and due to its stable and toxic nature, this material cannot be destroyed in the process of biological wastewater treatment. In this research, a new bacterial strain was isolated and purified from industrial wastewater for the biological removal of triclosan. Based on 16S rRNA sequencing, it was found that the isolated strain belongs to the Enterobacter cloacae family and can grow in high concentrations of triclosan. It was observed that naphthalene dioxygenase and laccase enzymes are active...