Loading...
Search for:
vosoughi--manochehr
0.01 seconds
Experimental Study of Partitioning of Lysozyme Enzyme in Aqueous Two-Phase Systems based on Surfactants
, M.Sc. Thesis Sharif University of Technology ; Bastani, Dariush (Supervisor) ; Vosoughi, Manochehr (Supervisor)
Abstract
In this study, aqueous two-phase systems (ATPS) containing Tween 20 as a biocompatible nonionic surfactant and three different salts (NH4HPO4, NH4CH3COO and Mg(CH3COO)2 ) were prepared and their extractive potential for Lysozyme enzyme was evaluated. These systems have surfactant-rich top phase and salt-rich bottom phase. Phase equilibrium diagrams at 23 ° C were obtained by using the cloud point method and the results were successfully fitted to the Merchuk correlation and their trends were correlated with the effective excluded volume (EEV) parameter. Afterward, the partitioning behavior of the lysozyme and its extraction efficiency in the systems of Tween 20/diammonium hydrogen phosphate,...
Experimental Study of Oily Wastewater Treatment by Hybrid Baffled Bioreactor
, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran (Supervisor) ; Vosoughi, Manochehr (Supervisor)
Abstract
Oil refineries, petrochemical factories and oil platforms produce a large amount of oily wastewater every day. If these wastewaters are released in environment, it can damage soil and water seriously. Although, Biological treatment of oily wastewaters is a well-established method for remediation of these wastes, efficiency of conventional systems such as Activated Sludge, aren’t very high. Also these systems have many problems such as instability during hydraulics shocks and bulking phenomena. the hybrid bioreactor system are designed to increase the efficiency of treatment by allowing greater organic loads, increasing microbial concentration by using attached and suspended bacteria, and...
Investigation of Activation Mechanism of Mechanosensitive Nano Ion Channels
, Ph.D. Dissertation Sharif University of Technology ; Vosoughi, Manochehr (Supervisor) ; Jamali, Yusef (Supervisor) ; Naghdabadi, Reza (Supervisor)
Abstract
Mechanosensitive (MS) channels are ubiquitous molecular force sensors that respond to a number of different mechanical stimuli including tensile, compressive and shear stress. One of the main mechanisms to functionally study these channels is the patch clamp technique. Here we use continuum mechanics to probe the question of how curvature, in a standard patch clamp experiment, at different length scales (global and local) affects a model MS channel. Firstly, to increase the accuracy of the Laplace’s equation in tension estimation in a patch membrane and to be able to more precisely describe the transient phenomena happening during patch clamping, we propose a modified Laplace’s equation. In...