Sharif Digital Repository

Enzymes are an important part of detergents, they reduce the activation energy of the reaction and thus increase the efficiency of the process. Protease enzyme is one of the most common enzymes in detergents. Bacillus strain is the most important strain in the production of this enzyme and among the species of Bacillus, Bacillus subtilis is the most used. Bacillus subtilis is a gram-positive, rod-shaped bacterium. This bacterium is considered a non-pathogenic cell that has the ability to form spores and prevent death and damage in harsh conditions. In this study, CRISPR system was used to modify the genome of Bacillus subtilis ATCC 6633 in order to increase alkaline protease. To increase the... 

Clustered Regularly Interspaced Short Palindromic Repeats, or in short, CRISPR is a relatively new technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding, or altering parts of the DNA. Initially found in the genomes of prokaryotic organisms such as bacteria and archaea, this technology can cure many illnesses such as blindness and cancer. A significant issue for a practical application of CRISPR systems is accurately predicting the single guide RNA (sgRNA) on-target efficacy and off-target sensitivity. While some methods classify these designs, most algorithms are on separate data with different genes and cells. The lack of... 

Genetic engineering of cells has been one of the most effective ways to increase protein production in wild strains. Among the various genetic engineering techniques, the CRISPR/Cas9 system that directly edits the cell chromosome, yielding greater efficiency and easier operation. In this study, we used this system to modify the genome of Bacillus subtilis ATCC 6633 to increase the production of extracellular alkaline protease enzyme. Alkaline protease enzyme has many applications in different industries, but in this study, the application of this enzyme in the detergent industry has been considered. To increase the production of this enzyme, we disrupted and prevented the gene responsible... 

Industrial enzymes can generally be classified into three groups: carbohydrases, proteases and lipases, which account for a major part of the global enzyme trade. Proteases, in particular, have always been extensively studied and investigated due to their widespread use in various industries, such as food, pharmaceutical, chemical, and agricultural sectors. Proteases are a group of proteolytic enzymes that break down proteins and peptides into their constituent amino acids by hydrolyzing peptide bonds. Among the family of proteases, alkaline serine proteases have special biocatalytic abilities in both aqueous and organic environments. Their suitable resistance in alkaline environments and...