Sharif Digital Repository

Simulating the in-vivo environment of cancer tumors outside the body, can provide an appropriate condition for studying different key processes of the tumor micro-environment, such as aggregation, formation, growth, angiogenesis and metastasis of the cancer tumor cells, which can be useful in finding methods to cure, or at least control cancer. Every year, a variety of drugs are being developed to overcome cancer, all of which need to be experimented. Although, these drugs are now experimented on animals, and some human volunteers, the reaction of different patients’ cancer cells to different drugs may differ with the experimented animals, or even with other people. So, there needs to be a... 

Cancer is one of the most causes of mortality in the world. Leukemia is a type of cancer initiated by the rapid proliferation of blood cells including WBCs, RBCs, and Platelets, that classified into four general types of AML, ALL, CML, and CLL. The main treatment of leukemia is chemotherapy. Because of many problems that patients have with this treatment and not so good results of chemotherapy, we design a microfluidic chip that can perform personalized medicine treatment for leukemia patients.This microchip consists of two main parts, Concentration Gradient Generator (CGG), and cell culture. CGG system is a tree CGG with two inlets and four outlets, which makes a linear concentration of... 

Nowadays, due to the growing need of the human to further improve the health of the community, research in the field of pharmacology as well as simulation of the cell culture environment is increasing. One of the most important requirements for simulating cell culture is the use of concentration gradient in culture media. The reason for this is the dependence of cellular activity on the presence of a gradient of concentration in the environment. Also, in drug investigations, in order to speed up the examination of different drugs and reduce the amount of drug that will used, different concentrations of the drug should be considered simultaneously in the microchips. Therefore, to make the... 

According to today’s medicine progress, the need for improving the medical facilities have been increased. Within the human body, the biomolecules concentration gradient is regulating the cell functions. Biological processes such as immune response, wound healing, and cancer metastasis have been affected by the bimolecular concentration gradient. So understanding the cell behavior in the presence of a chemical gradient can improve the understanding from these biological processes, and also would help in medical researches. On the other hand, finding the appropriate dose of the drugs and in some cases finding the most effective drug is a clinical challenge that made a new field of research in... 

It is of great interest to investigate the effects of different concentrations of chemical species on the behavior of test samples. For example, while developing a new drug, pharmacologists are concerned with the behavior of the live sample against different drug concentrations. Microfluidic systems are the new engineering platforms developed for simplifying these experiments. The main goal of the current study is the design and fabrication of a microfluidic system with the capability of generating a continuous concentration gradient from two species simultaneously on a millimeter-sized test sample. Such a system creates many benefits for researchers using microfluidics technology. Our... 

In this manuscript, we designed and fabricated a novel integrated microfluidic Kidney Nephron-On-Chip. This chip is able to culture cell monolayers under various fluid shear stresses and divert osmotic pressure gradients while imposing four different concentrations of an injected drug on cells. The multi-layer platform consisting of two bubble-trappers to eliminate all unwanted bubbles from the system, a concentration gradient generator to generate four different concentrations of the injected drug, and a membrane-based cell culture chamber caple of providing renal cells with their in-vivo condition. Using colorimetric techniques, the bubble trapper ability was quantified at flow rates up to...