Search for: glutathione
0.006 seconds

    Quantitative proteomic analysis of cellular responses to a designed amino acid feed in a monoclonal antibody producing Chinese hamster ovary cell line

    , Article Iranian Biomedical Journal ; Volume 22, Issue 6 , 2018 , Pages 385-393 ; 1028852X (ISSN) Torkashvand, F ; Mahboudi, F ; Vossoughi, M ; Fatemi, E ; Moosavi Basri, S. M ; Heydari, A ; Vaziri, B ; Sharif University of Technology
    Pasteur Institute of Iran  2018
    Background: Chinese hamster ovary (CHO) cell line is considered as the most common cell line in the biopharmaceutical industry because of its capability in performing efficient post-translational modifications and producing the recombinant proteins, which are similar to natural human proteins. The optimization of the upstream process via different feed strategies has a great impact on the target molecule expression and yield. Methods: To determine and understand the molecular events beneath the feed effects on the CHO cell, a label-free quantitative proteomic analysis was applied. The proteome changes followed by the addition of a designed amino acid feed to the monoclonal antibody producing... 

    Conformational aspects of glutathione tripeptide: Electron density topological & natural bond orbital analyses

    , Article Structural Chemistry ; Volume 24, Issue 1 , 2013 , Pages 147-158 ; 10400400 (ISSN) Aliakbar Tehrani, Z ; Fattahi, A ; Sharif University of Technology
    Glutathione tripeptide (γ-glutamyl-cysteinyl-glycine) is a flexible molecule and its conformational energy landscape is strongly influenced by forming intramolecular hydrogen bond, its charge and the environment. This study employs DFT-B3LYP method with the 6-31+G (d,p) basis set to carry out conformational analysis of neutral, zwitterionic, cationic, and anionic forms of glutathione. In analyzing the structural characteristics of these structures, intramolecular hydrogen bonds were identified and characterized in details by topological parameters such as electron density ρ(r) and Laplacian of electron density Δ2 ρ(r) from Bader's atom in molecules theory. Charge transfer energies based on... 

    Redox-sensitive smart nanosystems for drug and gene delivery

    , Article Current Organic Chemistry ; Volume 20, Issue 28 , 2016 , Pages 2949-2959 ; 13852728 (ISSN) Karimi, M ; Moosavi Basri, S. M ; Vossoughi, M ; Pakchin, P. S ; Mirshekari, H ; Hamblin, M. R ; Sharif University of Technology
    Bentham Science Publishers B.V 
    Background: Smart stimulus-responsive nanocarriers represent a rapidly growing class of drug-delivery systems for cancer and other diseases. Objective: The alterations of redox potential between the intracellular environment and the extracellular space have been widely utilized as a trigger for delivery of therapeutic agents by smart stimulus-responsive nanocarriers. We set out to review the scientific literature. Method: Published papers between 1980 and the present day were surveyed. Results: The advantages of redox-activated smart delivery of drugs, genes, and imaging agents include: the amplitude of the redox-responsive signal; the simple chemical features needed to trigger the... 

    Spectrophotometric Determination of Glutathione and Cysteine Based on Aggregation of Colloidal Gold Nanoparticles

    , M.Sc. Thesis Sharif University of Technology Seyyed Hosseini, Ensieh (Author) ; Hormozi Nezhad, Mohammad Reza (Supervisor)
    low-molecular-weigth aminothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play a critical role in many biochemical pathways . Their level in biological fluids such as human plasma and urine are important for clinical diagnostics of a variety of diseases.
    We report herein the development of a highly sensitive colorimetric method for detection of cysteine and glutathione using the citrate capped gold nanoparticles (Au-NPs). This thiol containing molecules exhibit intriguing reactivities with Au nanoparticles. The reactivity involves the displacement of the citrate shell by the thiolate shell which is followed by intermolecular electrostatic interactions or... 

    Wide color-varying visualization of sulfide with a dual emissive ratiometric fluorescence assay using carbon dots and gold nanoclusters

    , Article Microchemical Journal ; Volume 157 , September , 2020 Farahmand Nejad, M. A ; Bigdeli, A ; Hormozi Nezhad, M. R ; Sharif University of Technology
    Elsevier Inc  2020
    Development of visual detection methods regarding water pollutants can effectively facilitate water resource monitoring programs. A wide color-varying ratiometric approach has been developed for sensitive determination of sulfide by mixing orange emissive glutathione stabilized gold nanoclusters (AuNCs) and blue emissive urea stabilized carbon dots (CDs) in an appropriate ratio. Under a single excitation wavelength of 365 nm, the ratiometric fluorescence (RF) probe exhibited two distinct emission bands at 452 and 607 nm corresponding to CDs and AuNCs, respectively. Owing to the high affinity of sulfide ions to AuNCs in alkaline medium, the fluorescence (FL) of AuNCs was turned-off while the... 

    Interactions of glutathione tripeptide with gold cluster: Influence of intramolecular hydrogen bond on complexation behavior

    , Article Journal of Physical Chemistry A ; Volume 116, Issue 17 , 2012 , Pages 4338-4347 ; 10895639 (ISSN) Tehrani, Z. A ; Jamshidi, Z ; Javan, M. J ; Fattahi, A ; Sharif University of Technology
    Understanding the nature of the interaction between metal nanoparticles and biomolecules has been important in the development and design of sensors. In this paper, structural, electronic, and bonding properties of the neutral and anionic forms of glutathione tripeptide (GSH) complexes with a Au 3 cluster were studied using the DFT-B3LYP with 6-31+G**-LANL2DZ mixed basis set. Binding of glutathione with the gold cluster is governed by two different kinds of interactions: Auâ€"X (X = N, O, and S) anchoring bond and Au··•·•H-X nonconventional hydrogen bonding. The influence of the intramolecular hydrogen bonding of glutathione on the interaction of this peptide with the gold cluster has been... 

    Theoretical Investigation of Hydrogen Bonding Effects on Interaction of Metal Nanoclusters with Biomolecules & Acidity Enhancement of Alcohols

    , Ph.D. Dissertation Sharif University of Technology Ali Akbar Tehrani, Zahra (Author) ; Fattahi, Alireza (Supervisor) ; Mahmoodi Hashemi, Mohammad (Supervisor) ; Jamshidi, Zahra (Co-Advisor)
    This thesis is divided into three parts: In part I, conformational properties of neutral, anionic, cationic and zwitterionic forms of glutathione tripeptide were investigated by means of DFT-B3LYP method with 6-31+G (d,p) basis set. Results show that glutathione is a very flexible molecule and its conformational energy landscape is strongly influenced by forming intramolecular hydrogen bond and its charge. Conformation of each amino acid in glutathione tripeptide depends on its orientation within the peptide sequence in addition to the conformation of other amino acids within the chain. Investigation of intramolecular hydrogen bonds in these conformers by means of AIM analysis demonstrates... 

    Simultaneous Determination of Glutathione- Cysteine, and Detection of Mercury Ion (II) Based on Fluorescence Resonance Energy Transfer between Gold Nanoparticles and Fluorescein Isothiocyanate

    , M.Sc. Thesis Sharif University of Technology Mohammadi, Jamaloddin (Author) ; Hormozi-Nezhad, Mohammad Reza (Supervisor)
    Turn-On Fluorescence detection is a selective and sensitive method for many analytical purposes. Fluorescence quenching is a commonly observed consequence when fluorophores and quencher, gold nanoparticles (Au NPs) in this work, are in same media. Here, we report a “turn-on” fluorescent nanoprobe for simultaneous determination of glutathione and cysteine using H-point standard addition method (HPSAM). This probe develops based on the fluorescence resonance energy transfer (FRET) between Au NPs and fluorescein isothiocyanate (FITC), in which FITC acts as the donor and Au NPs as the acceptor. FRET causes the fluorescence intensity of FITC strictly quenched as a result of noncovalently adsorbed on... 

    Evaluation of the effects of fullerene c60 nanoparticles on oxidative stress parameters in normal rats liver and brain

    , Article Journal of Advances in Medical and Biomedical Research ; Volume 27, Issue 124 , 2019 , Pages 8-15 ; 26766264 (ISSN) Namadr, F ; Bahrami, F ; Bahri, Z ; Ghanbari, B ; Elahi, S. A ; Mohammadi, M. T ; Sharif University of Technology
    Zanjan University of Medical Sciences and Health Services  2019
    Background & Objective: The potent antioxidant property of fullerene C60 nanoparticles and their derivatives has been demonstrated in a wide range of in vitro and in vivo studies. Hence, we examined the effects of fullerene C60 on the oxidative stress parameters in brain and liver of the rats in normal situation. Materials & Methods: The study was performed in two groups of Wistar rats (each group, n = 6); normal and fullerene-treated normal animals. Treated rats received fullerene via oral gavage at dose of 1 mg/kg/day for 60 days. At termination of the study, the oxidative stress parameters were determined in brain and liver tissues, including the contents of glutathione (GSH) and... 

    A colorimetric sensor array for detection and discrimination of biothiols based on aggregation of gold nanoparticles

    , Article Analytica Chimica Acta ; Volume 882 , July , 2015 , Pages 58-67 ; 00032670 (ISSN) Ghasemi, F ; Hormozi-Nezhad, M.R ; Mahmoudi, M ; Sharif University of Technology
    Elsevier  2015
    Developments of sensitive, rapid, and cheap systems for identification of a wide range of biomolecules have been recognized as a critical need in the biology field. Here, we introduce a simple colorimetric sensor array for detection of biological thiols, based on aggregation of three types of surface engineered gold nanoparticles (AuNPs). The low-molecular-weight biological thiols show high affinity to the surface of AuNPs; this causes replacement of AuNPs' shells with thiol containing target molecules leading to the aggregation of the AuNPs through intermolecular electrostatic interaction or hydrogen-bonding. As a result of the predetermined aggregation, color and UV-vis spectra of AuNPs... 

    Association of glomerular and tubular dysfunction with glycaemic control, lipid, lipoprotein, apolipoprotein and antioxidant status in type 2 diabetes mellitus

    , Article Singapore Medical Journal ; Volume 48, Issue 9 , 2007 , Pages 840-846 ; 00375675 (ISSN) Farvid, M. S ; Djalali, M ; Siassi, F ; Farvid, S. S ; Sharif University of Technology
    Singapore Medical Association  2007
    Introduction: This study was conducted to investigate the relationship of glomerular and tubular dysfunctions with glycaemic control, lipid, lipoprotein, apolipoproteins and antioxidant status in 72 patients with type 2 diabetes mellitus. Methods: Urine albumin concentration was measured by immunoturbidimetric and urine N-acetyl-beta-D-glucosaminidase (NAG) and alanine aminopeptidase (AAP) activities with colorimetric methods. Glycated haemoglobin was measured using affinity chromatography. Erythrocyte glu ta thione reductase and glutathione peroxidase activities and serum levels of malondialdehyde, lipids, lipoproteins and apolipoproteins were determined in patients with type 2 diabetes... 

    Spectrophotometric determination of glutathione and cysteine based on aggregation of colloidal gold nanoparticles

    , Article Scientia Iranica ; Volume 19, Issue 3 , June , 2012 , Pages 958-963 ; 10263098 (ISSN) Hormozi Nezhad, M. R ; Seyedhosseini, E ; Robatjazi, H ; Sharif University of Technology
    We report herein the development of a highly sensitive colorimetric method for the determination of cysteine and glutathione, based on aggregation of the citrate capped gold nanoparticles (Au NPs). This was exploited from high affinity of low-molecular-weight aminothiols towards the Au NPs surface, which could induce displacement of the citrate shell by the thiolate shell of target molecules, resulting in aggregation of the NPs through intermolecular electrostatic interaction or hydrogen-bonding. As a result of aggregation, which can be affected by the ionic strength, pH and concentration of Au NPs, the plasmon band at around 521 nm decreases gradually, along with formation of a new red... 

    The metabolome profiling of obese and non-obese individuals: Metabolically healthy obese and unhealthy non-obese paradox

    , Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 186-194 Chashmniam, S ; Madani, N. H ; Ghoochani, B. F. N. M ; Safari Alighiarloo, N ; Khamseh, M. E ; Sharif University of Technology
    Mashhad University of Medical Sciences  2020
    Objective(s): The molecular basis of “metabolically healthy obese” and “metabolically unhealthy non-obese” phenotypes is not fully understood. Our objective was to identify metabolite patterns differing in obese (metabolically healthy vs unhealthy (MHO vs MUHO)) and non-obese (metabolically healthy vs unhealthy (MHNO vs MUHNO)) individuals. Materials and Methods: This case-control study was performed on 86 subjects stratified into four groups using anthropometric and clinical measurements: MHO (21), MUHO (21), MHNO (22), and MUHNO (22). Serum metabolites were profiled using nuclear magnetic resonance (NMR). Multivariate analysis was applied to uncover discriminant metabolites, and enrichment... 

    NMR spectroscopy-based metabolomic study of serum in sulfur mustard exposed patients with lung disease

    , Article Biomarkers ; Volume 22, Issue 5 , 2017 , Pages 413-419 ; 1354750X (ISSN) Nobakht, B.F., M. Gh ; Arefi Oskouie, A ; Rezaei Tavirani, M ; Aliannejad, R ; Taheri, S ; Fathi, F ; Naseri, M. T ; Sharif University of Technology
    Taylor and Francis Ltd  2017
    Sulfur mustard (SM) is a vesication chemical warfare agent for which there is currently no antidote. Despite years of research, there is no common consensus about the pathophysiological basis of chronic pulmonary disease caused by this chemical warfare agent. In this study, we combined chemometric techniques with nuclear magnetic resonance (NMR) spectroscopy to explore the metabolic profile of sera from SM-exposed patients. A total of 29 serum samples obtained from 17 SM-injured patients, and 12 healthy controls were analyzed by Random Forest. Increased concentrations of seven amino acids, glycerol, dimethylamine, ketone bodies, lactate, acetate, citrulline and creatine together with the... 

    Stimuli-responsive chitosan as an advantageous platform for efficient delivery of bioactive agents

    , Article Journal of Controlled Release ; Volume 317 , 2020 , Pages 216-231 Sabourian, P ; Tavakolian, M ; Yazdani, H ; Frounchi, M ; van de Ven, T. G. M ; Maysinger, D ; Kakkar, A ; Sharif University of Technology
    Elsevier B.V  2020
    Despite a diverse range of active pharmaceutical agents currently at our disposal, high morbidity rate diseases continue to pose a major health crisis globally. One of the important parameters in this regard is the controlled cargo delivery at desired sites. Among a variety of synthetic and natural macromolecular systems, chitosan, an abundant biopolymer, offers a platform for tailored architectures that could have high loading capacity of cargo, target and deliver. Stimuli directed accumulation of vehicles and drug release is an area of direct relevance to biomedical applications. In this review, we highlight essential characteristics of modified chitosan that present themselves for... 

    Engineering folate-targeting diselenide-containing triblock copolymer as a redox-responsive shell-sheddable micelle for antitumor therapy in vivo

    , Article Acta Biomaterialia ; Volume 76 , 2018 , Pages 239-256 ; 17427061 (ISSN) Behroozi, F ; Abdkhodaie, M. J ; Sadeghi Abandansari, H ; Satarian, L ; Molazem, M ; Al Jamal, K. T ; Baharvand, H ; Sharif University of Technology
    Acta Materialia Inc  2018
    The oxidation-reduction (redox)–responsive micelle system is based on a diselenide-containing triblock copolymer, poly(ε-caprolactone)-bis(diselenide-methoxy poly(ethylene glycol)/poly(ethylene glycol)-folate) [PCL-(SeSe-mPEG/PEG-FA)2]. This has helped in the development of tumor-targeted delivery for hydrophobic anticancer drugs. The diselenide bond, as a redox-sensitive linkage, was designed in such a manner that it is located at the hydrophilic–hydrophobic hinge to allow complete collapse of the micelle and thus efficient drug release in redox environments. The amphiphilic block copolymers self-assembled into micelles at concentrations higher than the critical micelle concentration (CMC)... 

    Inorganic nanomaterials for chemo/photothermal therapy: a promising horizon on effective cancer treatment

    , Article Biophysical Reviews ; Volume 11, Issue 3 , 2019 , Pages 335-352 ; 18672450 (ISSN) Khafaji, M ; Zamani, M ; Golizadeh, M ; Bavi, O ; Sharif University of Technology
    Springer Verlag  2019
    During the last few decades, nanotechnology has established many essential applications in the biomedical field and in particular for cancer therapy. Not only can nanodelivery systems address the shortcomings of conventional chemotherapy such as limited stability, non-specific biodistribution and targeting, poor water solubility, low therapeutic indices, and severe toxic side effects, but some of them can also provide simultaneous combination of therapies and diagnostics. Among the various therapies, the combination of chemo- and photothermal therapy (CT-PTT) has demonstrated synergistic therapeutic efficacies with minimal side effects in several preclinical studies. In this regard,... 

    Review on alzheimer's disease: inhibition of amyloid beta and tau tangle formation

    , Article International Journal of Biological Macromolecules ; Volume 167 , 2021 , Pages 382-394 ; 01418130 (ISSN) Ashrafian, H ; Hadi Zadeh, E ; Hasan Khan, R ; Sharif University of Technology
    Elsevier B.V  2021
    It is reported that approximately 40 million people are suffering from dementia, globally. Dementia is a group of symptoms that affect neurons and cause some mental disorders, such as losing memory. Alzheimer's disease (AD) which is known as the most common cause of dementia, is one of the top medical care concerns across the world. Although the exact sources of the disease are not understood, is it believed that aggregation of amyloid-beta (Aβ) outside of neuron cells and tau aggregation or neurofibrillary tangles (NFTs) formation inside the cell may play crucial roles. In this paper, we are going to review studies that targeted inhibition of amyloid plaque and tau protein tangle formation,... 

    Challenges and future prospects for the delivery of biologics: oral mucosal, pulmonary, and transdermal routes

    , Article AAPS Journal ; Volume 19, Issue 3 , 2017 , Pages 652-668 ; 15507416 (ISSN) Morales, J. O ; Fathe, K. R ; Brunaugh, A ; Ferrati, S ; Li, S ; Montenegro Nicolini, M ; Mousavikhamene, Z ; McConville, J. T ; Prausnitz, M. R ; Smyth, H. D. C ; Sharif University of Technology
    Springer New York LLC  2017
    Biologic products are large molecules such as proteins, peptides, nucleic acids, etc., which have already produced many new drugs for clinical use in the last decades. Due to the inherent challenges faced by biologics after oral administration (e.g., acidic stomach pH, digestive enzymes, and limited permeation through the gastrointestinal tract), several alternative routes of administration have been investigated to enable sufficient drug absorption into systemic circulation. This review describes the buccal, sublingual, pulmonary, and transdermal routes of administration for biologics with relevant details of the respective barriers. While all these routes avoid transit through the...