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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 42270 (06)
- University: Sharif University of Technology
- Department: Chemical and Petroleum Engineering
- Advisor(s): Yaghmaei, Soheyla; Abdekhodaie, Mohammad Jafar; Mehrvar, Mehrab
- Abstract:
- Kluyveromyces genus is known to be one of the important yeast used in processes of industrial biotechnology. This importance is due to production of Lactase, key enzyme in dairy industries that catalyses hydrolysis of lactose in milk. But high concentration of carbon source which is lactose in most cases, causes crab-tree effect, a metabolic pathway that leads to more production of ethanol rather than lactase. In this research the main purpose was to control the initial lactose concentration in medium to prevent decrease in enzyme activity. Controlled release system via Ethylene Vinyl Acetate tablets was chosen as a controller mechanism of lactose concentration as EVA was a well-known polymer as a sugar release agent. Two sets of experiments was done in normal shake flask and special controlled release systems and major parameters such as cell growth, lactose and ethanol concentration and enzyme activity was measured for further comparison. It was finally found that biomass concentration is initially higher in normal shake flask system and then it lowers to below other test’s results. Furthermore, ethanol production decreased significantly in controlled release system compared with other system and more enzyme activity was also gained. Ethanol concentration decline is due to decrease in production as well as consumption by cells
- Keywords:
- Intercellular Enzyme ; Kluyveromyces Fragilis ; CONTROLLED RELEASE ; Lactase Enzyme
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