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Bioremediation of Contaminated Soil by Hydrocarbon

Asgharzadeh, Ahmad | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42659 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Shaygan, Jalaloddin
  7. Abstract:
  8. This research was done in order to test the capability of endemic microorganisms in treating hydro-carbonic pollutants found in the soil specimens of Assaluye industrial region. To find the optimum conditions for treating the contaminations, we tried several amounts of substrate as well as temperature and humidity. The total concentration of hydrocarbon compounds in polluted soil samples was measured by both infrared spectrometry and gas chromatography, in treatment process after extraction. The results show that, having 50mg substrate per 1kg of contaminated soil added and keeping humidity at 11%, in a 60-days course the level of initial pollution decreased by 60%. 10 degrees increase in temperature, from 25℃ to 35℃, also resulted in 10% increase in treatment rate. Adding micro-nutrition like MgSO4 and FeSO4 also expedited treatment rate by 14%. The population of bacterial colonies capable of decomposing hydrocarbon compounds was also measured during the research by microbial culturing method. Adding substrate and regulating humidity and temperature in some bioreactors brought about up to one hundred times increase in bacterial population. The samples underwent an independent study for determining the possibility of bioremediation polluted soil, due to the sulfuric composition of some contaminations; A considerable portion of bacterial population (6 *10^4 cfu/g) found to be capable of decomposing sulfuric organic compounds
  9. Keywords:
  10. Oil Pollution ; Bioremediation ; Enhanced Bioremediation ; Biodesulfurization (BDS)

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