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Mortality response of folate receptor-activated, PEG-functionalized TiO2 nanoparticles for doxorubicin loading with and without ultraviolet irradiation

Naghibi, S ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ceramint.2013.10.136
  3. Abstract:
  4. TiO2 nanoparticles (NPs) were synthesized by hydrothermal assisted sol-gel technique. In the next step, as-synthesized NPs were modified by poly ethylene glycol (PEG). Then, folic acid (FA) was conjugated to TiO 2-PEG. Finally, Doxorubicin (Dox) as an anticancer drug was loaded on as-prepared TiO2-PEG-FA nanocarrier. The optimization of TiO 2 and FA concentration and the influence of ultraviolet (UV) irradiation on photocatalytic activity of nanocarrier and Dox loaded carrier were assessed by utilizing the 3-(4,5-dimethylthiazol-2yl)-2,5- diphenyltetrazolium bromide (MTT)-assay method. Results indicated that the effect of NPs on cancer cells killing is dose-dependent, optimized at 1.25 g/L. The performance of FA on the selective targeting was confirmed and maximized at FA/TiO2=0.5. As-optimized TiO2-PEG-FA-Dox conjugate, in comparison to pure Dox, was more effective as far as cancer cell viability reduced to about 5%, especially under UV irradiation
  5. Keywords:
  6. Cytotoxicity ; Doxorubicin ; MTT assay ; Photocatalytic activity ; TiO2-PEG-folic acid
  7. Source: Ceramics International ; Vol. 40, issue. 4 , May , 2014 , pp. 5481-5488 ; ISSN: 02728842
  8. URL: http://www.sciencedirect.com/science/article/pii/S0272884213014089