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Numerical Modeling of Endothelial Cell’s Groups Migration in a Microfluidics Device

Abeddoust, Mohammad | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46943 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir
  7. Abstract:
  8. Cell migration plays a key role in many biological processes including metastasis, wound healing, inflammatory response, body immune response and formation of new blood vessels. The migration of cells in response to the gradient of concentration gradient is regarded as chemotaxis. In the present study, the group chemotaxis of endothelial cells is simulated in response to gradient concentration of biochemical species using a developed cell migration model. At the first step, the numerical simulation of fluid flow and concentration transport of biochemical species is performed using a developed FVM code. At the second part, a model is developed to mimic the group migration of endothelial cells, in response to gradient concentration, using viscoelastic models for cell mechanics modeling. The developed model is more accurately tuned using available experimental observations using an alternative method. Using the developed cell migration model the influence of various parameters including cell’s group density, variation in the cell-cell interactions, and cytoskeletal orientation on the group migration of cells are investigated comprehensively in the presence and absence of biochemical concentration gradient, separately. It is noticed that increasing the cell density and so the cell-cell interactions in the presence of concentration gradient of biochemical species improves cell’s group migration in the direction of imposed concentration gradient. Moreover, investigating the orientation of cytoskeletal stress fibers reveals that alignment of stress fibers orientation in direction of imposed concentration gradient has a pivotal role in improvement of chemotaxis. In other words, as the cell-cell interactions are increased, the aliment of cytoskeletal stress fibers causes an intensified applied force on the cell in direction of migration. The obtained numerical results are also compared with available experimental observations that show a satisfactory agreement between the two results
  9. Keywords:
  10. Microfluidic Devices ; Viscoelastic Model ; Endothelial Cell ; Collective Cell Migration ; Numerical Cell Mechanics Modeling

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