Separation and Purification of Conjugated Linoleic Acid Isomers from Vegetable Oils

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Hajizadeh, Mohammad | 2017

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 50391 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Alemzadeh, Iran
Abstract:
Conjugated linoleic acid (CLA), a mixture of positional and geometrical isomers of linoleic acid with conjugated double bonds, has gained considerable attention because of its anticarcinogenic and obesity treatment properties. The most biologically active CLA isomers are 9c, 11t-18:2 and 10t, 12c-18:2. The aim of this study is to separate and purify the two essential isomers via enzymatic method by Candida rugosa lipase and chemical method by urea crystallization and also optimization of reaction conditions in order to better separation. For enzymatic purification, L-menthol and Lauryl alcohol were used separately. Optimization of reaction conditions by enzyme content, time and the ratio of free fatty acid to L-menthol or Lauryl alcohol was performed by using design-expert, TableCurve curve fitting and toolbox of genetic algorithm in Matlab. For chemical purification, solution of urea in methanol was used for crystallization. Purification by L-menthol obtained these results: 56% 9c,11t and 77% 10t,12c and purification by Lauryl alcohol obtained 58% 9c,11t and 66% 10t,12c. Purification by urea crystallization, obtained 32% 9c,11t and 65% 10t,12c.
Keywords:
Conjugated Linoleic Acid (CLA) ; Safflower Oil ; Purification ; Chemical Separation ; Chemical Production ; Chemical Purification

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