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- Type of Document: Ph.D. Dissertation
- Language: Farsi
- Document No: 51148 (08)
- University: Sharif University of Technology
- Department: Mechanical Engineering
- Advisor(s): Saeedi, Mohammad Saeed; Firoozabadi, Bahar; Mosadegh, Bobak
- Abstract:
- Renal diseases and consequently kidney failure is one of the important death factors around the world in recent years. It causes hundreds of thousands die every year and is in progress. Researchers believe that increasing chemical and nonchemical materials density in the blood which is the air pollution, use of tobacco and other parameters cause renal cells misfunction in urine filtration and regulation of blood pressure. The goal of this work is design and fabrication of a microfluidic device which provides different cell types to be cultured and imposed pressure. To do this, a high throughput microfluidic device is needed to mimic kidney biologically. Having such a device helps to analyze the cells function, proliferation and growth accurately. Thus, three-dimensional flow simulation software is utilized for design and microfabrication methods are used for fabrication of the device. Finally, the function of device has been verified by culturing the cells and circulating the blood as well. The results showed that the device has the capability of growth and proliferation of the endothelial and renal epithelial cells. Also, the results showed that the pressure doesn't affect cells viability both in static and dynamic cultures. The cells tight junctions weakened due to the pressure in static culture tests and consequently caused an increase of mass transfer from the tissue
- Keywords:
- Cell Culture ; Microfluidic Devices ; Organ on Chip ; Kidney Diseases ; Pressure Test Setup ; Kidney on Chip
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