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A simple 2-step purification process of α-amylase from bacillus subtilis: optimization by response surface methodology

Ataallahi, E ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2021.09.139
  3. Publisher: Elsevier B.V , 2021
  4. Abstract:
  5. Purification of extracellular α-amylase from Bacillus subtilis was carried out via fractional precipitation by acetone and ion exchange chromatography. These steps provide fast precipitation as well as purification of α-amylase to improve enzyme purity, activity and stability. Compared with two-phase methods in which the yield was less than 1, this method resulted in a yield of more than 3. Moreover, 95% of acetone was recovered that enhanced the economy of the downstream process. Using the data provided by 2D electrophoresis, purification was done by a single step ion exchange chromatography. The enzyme exhibited a molecular mass (SDS-PAGE) of 50KD and the pI of 5. Maximum “yield” and “purification fold” were achieved through optimization of operation parameters such as volume and flowrate of loaded protein using response surface methodology (RSM). 0.5ml of loaded protein at a flow rate of 0.5 ml/min was purified as 48 folds and achieved a specific activity of 524 U/mg. © 2021 Elsevier B.V
  6. Keywords:
  7. Acetone ; Amylase ; Bacillus subtilis ; Enzyme activity ; Enzyme purification ; Enzyme stability ; Flow rate ; Fractional precipitation ; Intermethod comparison ; Ion exchange chromatography ; Molecular weight ; Nonhuman ; Process optimization ; Protein folding ; Response surface method ; Volume
  8. Source: International Journal of Biological Macromolecules ; Volume 192 , 2021 , Pages 64-71 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813021020675