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Inhibitory activity on type 2 diabetes and hypertension key-enzymes, and antioxidant capacity of veronica persica phenolic-rich extracts
, Article Cellular and Molecular Biology ; Volume 62, Issue 6 , 2016 , Pages 80-85 ; 01455680 (ISSN) ; Tayeboon, G. S ; Sharifi Rad, J ; Iriti, M ; Varoni, E. M ; Razazi, S ; Sharif University of Technology
Cellular and Molecular Biology Association
2016
Abstract
Veronica genus (Plantaginaceae) is broadly distributed in different habitats. In this study, the inhibitory activity of free soluble and conjugated phenolic extracts of Veronica persica on key enzymes associated to type 2 diabetes (α-glucosidase and α-amylase) and hypertension (angiotensin I converting enzyme, ACE) was assessed, as well as their antioxidant power. Our results showed that both the extracts inhibited α-amylase, α-glucosidase and ACE in a dose-dependent manner. In particular, free phenolic extract significantly (P < 0.05) inhibited α-glucosidase (IC50 532.97 μg/mL), whereas conjugated phenolic extract significantly (P < 0.05) inhibited a-amylase (IC50 489.73 μg/mL) and ACE...
Simulation and Optimization of α-amylase Enzyme Purification Process
Using Column Chromatography
,
M.Sc. Thesis
Sharif University of Technology
;
Rosstaazad, Reza
(Supervisor)
Abstract
Enzymes, which are biochemical catalysts, have important applications in different industries such as food, pharmaceutical, medical, chemical identification and etc. Several methods have been used for separation of enzymes among which precipitation, chromatography and two-phase system. In this work, purification of α-amylase has been done by Ammonium Sulfate and Acetone precipitation, gel filtration based on Sephadex, and PEG-salt aqueous two-phase system. Furthermore, mass and momentum conservations’ equations were solved simultaneously to simulate the process based on the finite element method (FEM). Crude enzyme was precipitated with different saturations of ammonium sulfate and the...
Studing the Performance of Rotary Dryer in Drying Enzyme Products
, M.Sc. Thesis Sharif University of Technology ; Roosta Azad, Reza (Supervisor)
Abstract
Drying is one of the important downstream processes in the production of biotechnological products. The dryer design for heat-sensitive materials such as proteins requires special process considerations. In this study, a rotary dryer evaluated for drying protein products. The used liquid enzyme as model, a bacterial α-amylase, first, immobilized on sodium sulfate and sodium tripolyphosphate as carriers in a spraying mode and then, the obtained wet solid materials containing the enzyme, dried in a batch dryer. The performance of this dryer investigated to achieve suitable properties such as the enzyme activity and the final moisture content of product by changing the process conditions....
Determination of Optimum Temperature Profile in Fermentation of B.Subtilis to Maximize Alpha-Amylase Activity
, M.Sc. Thesis Sharif University of Technology ; Vosoughi, Manouchehr (Supervisor) ; Roostaazad, Reza (Supervisor)
Abstract
Alpha amylase enzyme is one of the most important enzymes used in a wide range of industries. The enzyme is used in industries such as detergents, food, pharmaceuticals, paper making and textile. Various national and international articles have been published on this enzyme that focused on specific targets. Despite the extensive studies carried out in this area inside the country, there is still no industrial production of alpha amylase and other enzymes in our country. In the view of the fundamental problem of the lack of industrialization of these studies, in addition to the lack of a riskier investor, it seems that there is a lack of industrial vision in these studies.In this study, the...
Design and Operation of Proper Freeze Drying Process to Recover Active Biological Agent
, M.Sc. Thesis Sharif University of Technology ; Rosta Azad, Reza (Supervisor)
Abstract
One of the most important unit operations in the production of dry powder biological products involves a freeze dryer apparatus. In this process, Liqueur, which is containing the α-amylase enzyme, undergoes a frigidity condition in which loses its moisture during sublimation. Under these circumstances, applying an adequate supply of sublimation enthalpy without raising the temperature of the frigid material up to melting, plays an important role in the quality of the product and preventing degradation of the α-amylase enzyme. Freeze drying depends on two main factors; time and energy. Furthermore, it is necessary to obtain an optimal temperature and pressure for different steps and...
Studying the Effective Factors and Optimizing Enzyme (α-amylase) Separation in Multi-Enzyme Systems by Agarose Column Chromatography
, Ph.D. Dissertation Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Soheila Yaghmaee (Supervisor) ; Yeganeh Sarkandi, Shahin (Co-Supervisor)
Abstract
In all types of chromatography for the extraction and purification of proteins, a fixed substrate is needed with which the mobile phase containing the proteins interact with them. This fixed bed should contain grains that have characteristics such as sphericity, high surface area, hydrophilicity, binding power to functional groups, chemical stability, low specific absorption, reusability and low cost. Commercially, the most commonly substrates in the ranges of the molecular weight of proteins are agarose and dextran. Agar was extracted from Gracilaria salicornia seaweed and removal of ionic groups was investigated by three methods. In washing with isopropanol, the ionic groups were reduced...
Investigating the Performance of Fluidized bed Dryer in Immobilization and Drying of A-amylase Enzyme
, M.Sc. Thesis Sharif University of Technology ; Roostaazad, Reza (Supervisor)
Abstract
Enzyme immobilization/drying operations are among the important operations of many biotechnological industrial units. The enzyme used in this study is bacterial alpha-amylase, which is widely used in various industries, including detergents. The production of alpha-amylase enzyme contains a large percentage of water in its composition; therefore, a wet wall tower was designed to conduct the experiments to evaporate the water present in the enzyme liquor, analyze its effects on activity, and also to validate the device. The results showed that in a flow of air around 240m3/hr and ambient temperature of 20◦C, enzyme liquor has a 250% increase in biological activity, which indicates the...
α-Amylase Immobilization on Nanocomposite Magnetic Silica Particles and Characterization of the Prepared Nanobiocatalysts
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Arpanaei, Ayyoob (Supervisor)
Abstract
Enzymes are widely used in industry, however, the expensive cost, low stability, and the limited activity in a certain range of temperature and pH, have limited the usage of such bio-catalists. In addition to increasing and preserving their activity under different conditions, the enzyme immobilization can improve its stability in different pH, temperature values and increase its thermostability, storage stability and the possibility of their recycling. Meanwhile, utilizing the super-magnetic systems could be very helpful as well. For instance, separation of the the magnetic nano-particles like Fe3O4 from reaction system using an external magnetic field is easily possible. In this study,...
studying the Effect of Adding Nanoparticles in Polymer/salt Aqueous Two Phase Systems for Separating of Alpha-amylase Enzyme
,
Ph.D. Dissertation
Sharif University of Technology
;
Vossoughi, Manoochehr
(Supervisor)
;
Pazuki, Gholamreza
(Supervisor)
Abstract
The effects of adding nanoparticles in partitioning of α-amylase in polymer/salt aqueous two phase system (ATPS) was studied in this research. First the binodal curves of magnesium sulfate salt with PEGs 2000,3000,4000,6000 and 10000 at 23 ° C has been plotted and the suitable system which was PEG 6000/ magnesium sulfate has been selected for studying the partitioning of enzyme. Thereupon the partition coefficient of α-amylase was measured and then effect of adding silica and graphene oxide nanoparticles was investigated. It was observed that nanoparticles caused a dramatic increase in partition coefficient and yield recovery (of top phase). For further investigation the partitioning of...
A simple 2-step purification process of α-amylase from bacillus subtilis: optimization by response surface methodology
, Article International Journal of Biological Macromolecules ; Volume 192 , 2021 , Pages 64-71 ; 01418130 (ISSN) ; Naderi-Manesh, H ; Roostaazad, R ; Yeganeh, S ; Sharif University of Technology
Elsevier B.V
2021
Abstract
Purification of extracellular α-amylase from Bacillus subtilis was carried out via fractional precipitation by acetone and ion exchange chromatography. These steps provide fast precipitation as well as purification of α-amylase to improve enzyme purity, activity and stability. Compared with two-phase methods in which the yield was less than 1, this method resulted in a yield of more than 3. Moreover, 95% of acetone was recovered that enhanced the economy of the downstream process. Using the data provided by 2D electrophoresis, purification was done by a single step ion exchange chromatography. The enzyme exhibited a molecular mass (SDS-PAGE) of 50KD and the pI of 5. Maximum “yield” and...
Prediction of the partition coefficients of biomolecules in polymer-polymer aqueous two-phase systems using the artificial neural network model
, Article Particulate Science and Technology ; Volume 28, Issue 1 , 2010 , Pages 67-73 ; 02726351 (ISSN) ; Taghikhani, V ; Vossoughi, M ; Sharif University of Technology
Abstract
In this work, an artificial neural network model was used to obtain the partition coefficients of biomolecules in polymer-polymer aqueous two-phase systems. In the artificial neural network, the partition coefficient of a biomolecule depends on the difference between concentrations of poly (ethylene glycol), dextran in the top and bottom phases, temperature and molecular weights of poly (ethylene glycol), dextran, and the biomolecule. The network topology is optimized and the (6-1-1) architecture is found using optimization of an objective function with sequential quadratic programming (SQP) method for 450 experimental data points. The results obtained from the neural network of the...
Influence of glycerol and clay contents on biodegradability of corn starch nanocomposites
, Article International Journal of Engineering, Transactions B: Applications ; Vol. 27, issue. 2 , 2014 , pp. 203-214 ; ISSN: 10252495 ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
Abstract
In this study, biodegradation of corn starch/glycerol/Na-MMT nanocomposites by soil burial tests were carried out for up to 6 months. Films were prepared by casting method according to central composite design and response surface methodology was applied for analysis of the results. The effects of formulation on the water solubility, contact angle and tensile strength of starch films were investigated. Increasing in Na-MMT content decreased biodegradability; while presence of glycerol, increased it. The effect of nanoparticles content might have been attributed to interactions between starch and Na-MMT that further prevented enzymatic attack on the biopolymer. In addition, the films with...
Measurement of partition coefficients of β-amylase and amyloglucosidase enzymes in aqueous two-phase systems containing poly(ethylene glycol) and Na2SO4/KH2PO4 at different temperatures
, Article Fluid Phase Equilibria ; Volume 292, Issue 1-2 , May , 2010 , Pages 80-86 ; 03783812 (ISSN) ; Taghikhani, V ; Vossoughi, M ; Safe kordi, A. A ; Alemzadeh, I ; Pazuki, G. R ; Sharif University of Technology
2010
Abstract
In this research, partitioning of β-amylase and amyloglucosidase was investigated in polymer-salt aqueous two-phase systems containing poly(ethylene glycol) (PEG) with different molecular weights such as 4000, 6000 and 10,000 and Na2SO4 or KH2PO4. The experimental partition coefficients were measured at T = (298.5, 301.5, 303.5, 305.5 and 308) K. The results showed that the concentration of salt in feed had more effect on partition coefficients of β-amylase and amyloglucosidase in aqueous two-phase systems than the concentration of polymer in initial feed. Also, the results indicated that temperature had a small effect on partitioning of β-amylase and amyloglucosidase enzymes. The partition...