Loading...

Preparation of Electro Conductive-piezoelectric Nanocomposite for Cartilage Tissue Engineering

Bagheri, Lida | 2020

204 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 54921 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Ramazani, Ahmad; Nasrollahzadeh, Naser
  7. Abstract:
  8. Articular cartilage resides in a complex and dynamic mechanical and biophysical environment in vivo. Chondrocyte physiology and biosynthetic activity is influenced by these signals, allowing them to modulate the structural organization and function of tissue at all stages, including development, growth and repair. . In articular cartilage, during physical activity and mechanical loading, electric signal have been observed in form of stress-generated electric potential following the movement of the positive mobile ions (in interstitial fluid) away from the fixed negative charges.Despite increasing evidence that mechanical and electrical stimuli have positive effects on chondrogenesis, to date, the coupling effect of them has received a very limited attention. Designing a 3D biomaterials with relatively stiff structure and capable for simulating the electrical signals of native cartilage is central to study cartilage electro-mechanobiology (stress-generated electric potential) in vitro. To address this challenge, we designed a 3D piezo composite hydrogel with electro-mechanical properties within the range of cartilage. Despite traditional method used for in vitro electrical stimulation which are not biomimetic (e.g., using an external power supply), we employed electrospun piezoelectric polyvinylidene nanofibers in the hydrogel structure as an electroactive phase to generate electric signals upon mechanical loading. We showed that incorporation of nanofibers into hydrogel networks significantly increase their mechanical properties and enhance cells adhesion and proliferation inside the composite constructs thanks to the implemented nano-topographic structure. It was observed that piezoelectric effects promote cells growth and metabolic activity compared to respective control groups. The expression of chondrogenic markers following applied stimulation was also higher for the piezo-composite hydrogel compared to the pure hydrogels. This approach allowed us to evaluate the synergistic effect of electro-mechanical cues on chondrocyte biological response
  9. Keywords:
  10. Cartilage Tissue Engineering ; Hydrogel ; Piezoelectric Materials ; Three Dimentional Electrospun ; Fiber Reinforced Hydrogels ; Electromechanical Signals ; Polymer Nanocomposits

 Digital Object List

 Bookmark

No TOC