Search for: cartilage-tissue-engineering
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    Dosign and Construction of Natural Polymers-based Hydrogels for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Nasiri, Bita (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hajebrahimi, Zahra (Supervisor)
    Once damaged, articular cartilage has very little capacity for spontaneous healing because of the avascular nature of the tissue. Although many repair techniques have been proposed over the past decades, none has successfully regenerated long-lasting tissue to replace damaged cartilage. Tissue engineering have recently demonstrated tremendous approaches for regeneration of cartilage tissue lesions. Tissue engineering is based on three principles: cells, scaffolds for cell adhesion and growth factors. Three-dimensional biodegradable scaffolds play an important role in tissue engineering. In this study, novel cross-linked hybrid chitosan/ECM scaffolds were prepared for articular cartilage... 

    Design and Fabrication of Scaffold Composed of Acellular Cartilage Matrix and Chitosan

    , M.Sc. Thesis Sharif University of Technology Sivandzade, Farzane (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hasanzadeh, Zabihollah (Supervisor)
    Cartilage is an avascular tissue, having limited ability to repair itself. Since the methods for treatment of cartilage defects have been not effective, in recent years, new therapies based on tissue engineering are considered.This paper reports on the development of porous microcarriers composed of acellular matrix of cartilage and natural polymer chitosan. Microcarriers were prepared by electrospray method. Results of mechanical tests, SEM imaging, water uptake behaviour, biodegradation test, and MTT assay demonstrated that the microcarriers composed of 2% (wt) chitosan and 1% (wt) ECM has the best potential for growth and proliferation of primary chondrocyte cells. These results... 

    Synthesis and Characterization of ATPEG-PMDA Hydrogels Conjugated with Thermo-Responsive Coated Magnetic Nanoparticles for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Abdorahim, Marjan (Author) ; Simchi, Abdoreza (Supervisor)
    A novel thermo responsive decorated system is designed by coating Fe3O4 magnetic nanoparticles with conjugated Pluronic-ATPEG copolymer incorporated into the PEG-PMDA Hydrogel for drug delivery to the cartilage tissue. Grafted copolymer was synthesized by reaction between carboxylated Pluronic and amino terminated Poly ethylene glycol (ATPEG) and confirmed by FTIR and NMR analysis. The magnetic nanoparticles were modified with the produced copolymer and characterized by TEM, HRTEM, XRD, DLS, and VSM. A typical product has 13 nm magnetic core and 105 nm hydrodynamic diameter with narrow size distribution. DLS results showed that there was an increase in size by increasing temperature from 25℃... 

    Fabrication of in Situ Forming Bioadhesive Hydrogel for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Abdolmaleki, Hamid (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hasanzadeh, Zabihollah (Supervisor)
    In recent years, many efforts have been made in tissue engineering and new methods for the treatment of cartilage damage, with an emphasis on their non-invasive and less aggressive nature. Meanwhile, injectable and in situ forming hydrogels have been considered as a less invasive nature. On the other hand, lack of enough mechanical properties in these hydrogels is one of their main problem. In this study, gelatin and alginate was used to fabricate hydrogel as interpenetrating network (IPN) hydrogel and silica nano particles were also used to increase mechanical properties in the fabricating of hydrogels. Gelatin is also combined with dopamine in order to induce bio adhesive properties of... 

    Smart polymeric hydrogels for cartilage tissue engineering: A review on the chemistry and biological functions

    , Article Biomacromolecules ; Volume 17, Issue 11 , 2016 , Pages 3441-3463 ; 15257797 (ISSN) Eslahi, N ; Abdorahim, M ; Simchi, A ; Sharif University of Technology
    American Chemical Society 
    Stimuli responsive hydrogels (SRHs) are attractive bioscaffolds for tissue engineering. The structural similarity of SRHs to the extracellular matrix (ECM) of many tissues offers great advantages for a minimally invasive tissue repair. Among various potential applications of SRHs, cartilage regeneration has attracted significant attention. The repair of cartilage damage is challenging in orthopedics owing to its low repair capacity. Recent advances include development of injectable hydrogels to minimize invasive surgery with nanostructured features and rapid stimuli-responsive characteristics. Nanostructured SRHs with more structural similarity to natural ECM up-regulate cell-material... 

    Fabrication of Composite Scaffold Composed of Cartilage Extracellular Matrix/Chitosan with High Mechanical Strength for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Khozaei Ravari, Mojtaba (Author) ; Mashayekhan, Shohreh (Supervisor) ; Baghban Eslami Nejad, Mohammad Reza (Supervisor)
    Methods that has been used for articular defects are faced with many limitations, so new therapies based on tissue engineering were taken into consideration in recent years. However, tissue engineering also encounters challenges regarding optimal scaffold construction and suitable cell source selection. Mature harvested chondrocytes are limited in number and may lose their chondrogenic potential in several cultures, leading to dedifferentiation. In addition, using stem cells also presents unique challenges associated with them, among which hypertrophic differentiation is the most substantial problem. Choosing the appropriate biomaterial similar to the cartilage structure with sufficient... 

    Hybrid System for Growth Factor Delivery

    , M.Sc. Thesis Sharif University of Technology Torabi Rahvar, Parisa (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)
    Platelet Rich Plasma (PRP) is a blood-derived product containing concentrate of platelets, which are a rich source of autologous growth factors. PRP injection has been used clinically as a therapeutic method for cartilage repair. However, clinical efficiency of this method is unpredictable, maybe as a result of burst release of growth factors then fail cell-stimulating potential as most biomolecules are cleaned before they can exert a therapeutic effect. The aim of this project was to prepare a suitable scaffold for PRP delivery to regenerate cartilage injuries. In order to resemble polysaccharide-protein nature of the cartilaginous extracellular matrix, in this study, we developed an... 

    Combination of PRP (platelet Rich plasma)with Polymeric Scaffold for Cartilage Regeneration

    , M.Sc. Thesis Sharif University of Technology Rafiei, Mehrnoosh (Author) ; Abdekhodaei, Mohammad Jafar (Supervisor)
    The aim of this study is the fabrication of 3D porous PCL scaffolds contain core-shell fibers for cartilage tissue engineering. The novel fabrication method is co-axial wet electrospinning simultaneously. These tablets like scaffolds have superior porosity and pore sizes for cell culturing and cell-cell interaction as they have good mechanical properties for cartilage tissue engineering in comparison to 2D electrospun scaffolds. The structure of these 3D scaffolds is mimicking the ECM of cartilage. This study presents the coaxial electrospinning of PCL nanofibers encapsulated with bovine serum albumin and platelet rich plasma for demonstration of controlled release and bioactivity retention,... 

    Fabrication of in Situ Forming Hydrogels Composed of Acellular Cartilage Matrix with Improved Mechanical Properties for Recovery of Local Damages in Articular Cartilage

    , M.Sc. Thesis Sharif University of Technology Shojarazavi, Nastaran (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hassanzadeh, Zabihollah (Co-Supervisor)
    Since cartilage has limited self-regeneration, in-situ forming hydrogels can act as an ideal scaffold for cartilage tissue engineering to fill the defect gap due to their ability to homogeneously encapsulate the desired cells, efficient mass transfer and minimally invasive characteristics. In this project, an injectable hydrogel with improved structure by adding silk fibroin (SF) nanofibers and better biochemical properties by employing the cartilage extracellular matrix (ECM) was fabricated. The in-situ forming hydrogel is consisted of different concentrations of ionic crosslinked alginate incorporated with different concentrations of SF nanofibers and 1% w/v enzymatically crosslinked... 

    Design and Fabrication of Three Dimensional Scaffold Using Bio-Printing Technique for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Mohsenifard, Sadaf (Author) ; Mashayekhan, Shohreh (Supervisor) ; Hassanzadeh, Zabiollah (Co-Supervisor)
    Herein, we successfully prepared two types of hydrogels, alginate-beta-cyclodextrin based hydrogel and alginate without beta-cyclodextrin hydrogel in order to use in cartilage tissue engineering. At first, sodium alginate was modified with beta-cyclodextrin ring due to incorporate the kartogenin (KGN) differentiating drug in the hydrogel structure. For confirming the correctness of the modification reactions, H-NMR spectroscopy was used. In the presence of phenolized-ECM, alginic acid, modified alginate with beta cyclodextrin, HRP, H2O2, calcium carbonate, and glucono-delta-lactone (GDL), ECM-alginate-IPN and ECM-modified-alginate-IPN hydrogels were synthesized. Then, the KGN was loaded on... 

    Preparation of Electro Conductive-piezoelectric Nanocomposite for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Bagheri, Lida (Author) ; Ramazani, Ahmad (Supervisor) ; Nasrollahzadeh, Naser (Co-Supervisor)
    Articular cartilage resides in a complex and dynamic mechanical and biophysical environment in vivo. Chondrocyte physiology and biosynthetic activity is influenced by these signals, allowing them to modulate the structural organization and function of tissue at all stages, including development, growth and repair. . In articular cartilage, during physical activity and mechanical loading, electric signal have been observed in form of stress-generated electric potential following the movement of the positive mobile ions (in interstitial fluid) away from the fixed negative charges.Despite increasing evidence that mechanical and electrical stimuli have positive effects on chondrogenesis, to... 

    Design of a Double-Network Hydrogel Scaffold for Cartilage Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Ganjali, Amir Reza (Author) ; Abdekhodaei, Mohammad Jafar (Supervisor)
    Osteoarthritis has always been one of the most common diseases in middle age because it causes severe pain and inflammation in the joints of the body. Cartilage tissue does not have the ability to repair itself. For this reason, fabricating and designing the most efficient, least-expensive, and most convenient methods for the treatment of cartilage defects is always an important issue. Today, there are surgical and injectable methods to relieve pain and initiate the body's natural healing response. But due to the many disadvantages and limitations of these methods, tissue engineering science has turned to modifying these methods or providing new methods. One of these methods is the use of... 

    The impact of zirconium oxide nanoparticles content on alginate dialdehyde-gelatin scaffolds in cartilage tissue engineering

    , Article Journal of Molecular Liquids ; Volume 335 , 2021 ; 01677322 (ISSN) Ghanbari, M ; Salavati Niasari, M ; Mohandes, F ; Firouzi, Z ; Mousavi, S.-D ; Sharif University of Technology
    Elsevier B.V  2021
    The desire to regenerate and repair native tissues can be immediately performed by multiple tissue engineering procedures. Gelatin and alginate are biocompatible and biodegradable polymers. The addition of ZrO2 nanoparticles (NPs) into the alginate-gelatin hydrogel is considered to improve mechanical and chemical properties. Therefore, nanocomposite hydrogels have been manufactured by the freeze-drying procedure utilizing oxidized alginate-gelatin with ZrO2 NPs as a reinforcement. The fabricated nanocomposite hydrogels were character-ized by FTIR, FESEM, and rheometer. The hydrogels containing a higher ZrO2 NPs content (1.5%) have better mechanical properties than the hydrogels without NPs.... 

    Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering

    , Article Journal of Biomaterials Applications ; Volume 36, Issue 5 , 2021 , Pages 803-817 ; 08853282 (ISSN) Shojarazavi, N ; Mashayekhan, S ; Pazooki, H ; Mohsenifard, S ; Baniasadi, H ; Sharif University of Technology
    SAGE Publications Ltd  2021
    In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was... 

    Design and Manufacture of a Scaffold with a Drug Delivery System for a Better Tissue Wound Healing Process

    , M.Sc. Thesis Sharif University of Technology Shaygani, Hossein (Author) ; Shamloo, Amir (Supervisor) ; Aryanpour, Masoud (Supervisor)
    Articular cartilage is devoid of blood vessels, lymphatics, and nerves which gives it a very limited intrinsic healing and repair capabilities. Being under constant harsh biomechanical environment, makes maintaining the health of articular cartilage a vital principle in having healthy joints. Tissue engineering as a method for regeneration of damaged tissue have attracted a lot of attention. Articular cartilage engineered scaffolds act as a macro scale drug delivery system which in addition to having a good mechanical properties similar to that of cartilage tissue, have to provide a highly porous environment for cell migration and proliferation. The aim of this study is to fabricate a drug... 

    Fabrication of porous scaffolds with decellularized cartilage matrix for tissue engineering application

    , Article Biologicals ; Volume 48 , 2017 , Pages 39-46 ; 10451056 (ISSN) Nasiri, B ; Mashayekhan, S ; Sharif University of Technology
    Academic Press  2017
    Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM. The... 

    Injectable polyethylene glycol-laponite composite hydrogels as articular cartilage scaffolds with superior mechanical and rheological properties

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 66, Issue 3 , 2017 , Pages 105-114 ; 00914037 (ISSN) Nojoomi, A ; Tamjid, E ; Simchi, A ; Bonakdar, S ; Stroeve, P ; Sharif University of Technology
    Taylor and Francis Inc  2017
    In this study, injectable PEG-based hydrogels containing Laponite particles with mechanical and structural properties close to the natural articular cartilage are introduced. The nanocomposites are fabricated by imide ring opening reactions utilizing synthesized copolymers containing PEG blocks and nanoclay through a two-step thermal poly-(amic acid) process. Butane diamine is used as nucleophilic reagent and hydrogels with interconnected pores with sizes in the range of 100–250 µm are prepared. Improved viscoelastic properties compared with the conventional PEG hydrogels are shown. Evaluation of cell viability utilizing human mesenchymal stem cells determines cytocompatibility of the... 

    Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 67, Issue 1 , 2018 , Pages 50-60 ; 00914037 (ISSN) Zamanlui, S ; Mahmoudifard, M ; Soleimani, M ; Bakhshandeh, B ; Vasei, M ; Faghihi, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    The simultaneous effect of electrospun scaffold alignment and polymer composition on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMMSC) is investigated. Aligned and randomly oriented polycaprolactone/poly(lactic-co-glycolic acid) (PLGA) hybrid electrospun scaffolds with two different ratios are fabricated by electrospinning. It is found that aligned nanofibrous scaffolds support higher chondrogenic differentiation of hBMMSCs compared to random ones. The aligned scaffolds show a higher expression level of chondrogenic markers such as type II collagen and aggrecan. It is concluded that the aligned nanofibrous scaffold with higher PLGA ratio could significantly... 

    Construction of 3D fibrous PCL scaffolds by coaxial electrospinning for protein delivery

    , Article Materials Science and Engineering C ; Volume 113 , 2020 Rafiei, M ; Jooybar, E ; Abdekhodaie, M. J ; Alvi, M ; Sharif University of Technology
    Elsevier Ltd  2020
    In this study, a three-dimensional tablet-like porous scaffold, comprising core-shell fibers to host proteins inside the core, was developed. The fabrication method involved the novel combination of coaxial and wet electrospinning in a single setting. Poly (ε-caprolactone) was chosen as the based polymer and bovine serum albumin was used as a model protein. These 3D tablet-like scaffolds exhibited adequate porosity and suitable pore size for cell culture and cell infiltration, in addition to appropriate mechanical properties for cartilage tissue engineering. The effects of different parameters on the behavior of the system have been studied and the 3D scaffold based on the core-shell fiber... 

    Fabrication of a novel 3D scaffold for cartilage tissue repair: In-vitro and in-vivo study

    , Article Materials Science and Engineering C ; Volume 128 , 2021 ; 09284931 (ISSN) Haghighi, P ; Shamloo, A ; Sharif University of Technology
    Elsevier Ltd  2021
    Self-repairing is not an advanced ability of articular cartilage. Tissue engineering has provided a novel way for reconstructing cartilage using natural polymers because of their biocompatibility and bio-functionality. The purpose of cartilage tissue engineering is to design a scaffold with proper pore structure and similar biological and mechanical properties to the native tissue. In this study, porous scaffolds prepared from gelatin, chitosan and silk fibroin were blended with varying ratios. Between the blends of chitosan (C), gelatin (G) and silk fibroin (S), the scaffold with the weight per volume ratio of 2:2:3 (w/v) showed the most favorable and higher certain properties than the...