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Characterization and determination of fatty acids in fish oil using gas chromatography-mass spectrometry coupled with chemometric resolution techniques

Jalali Heravi, M ; Sharif University of Technology | 2004

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  1. Type of Document: Article
  2. DOI: 10.1016/j.chroma.2003.10.032
  3. Publisher: Elsevier , 2004
  4. Abstract:
  5. Characterization and determination of a complex mixture of fatty acid methyl esters was performed for commercial fish oil using two-dimensional GC-MS data coupled with resolution techniques. Various principle component analysis methods such as significant factor analysis and fixed size moving window evolving factor analysis were used for the number of factors, zero concentration and selective regions. Then, the convoluted chromatograms were resolved into pure chromatograms and mass spectra using heuristic evolving latent projections (HELP) method. Fatty acids of C16:1ω7, C18:4ω3, C18:1ω11, C18:1ω9, C18:0, C20:2ω6, C20:1ω9, C 22:1ω11, C22:1ω9 and C24:1ω9 were resolved and identified by using similarity searches between deconvoluted mass spectra and MS database, in different parts of total ion current chromatogram. Window target testing factor analysis is also applied for confirming the presence or absence of target analytes. The results of the present work show that combination of hyphenated chromatographic methods and resolution techniques provide a complementary method for accurate analysis of real multi-component systems such as fish oil. © 2003 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Chemometrics ; Curve resolution ; Fatty acid methyl esters ; Oils ; Target testing
  8. Source: Journal of Chromatography A ; Volume 1024, Issue 1-2 , 2004 , Pages 165-176 ; 00219673 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0021967303018892