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Co-Immobilization of Multi-Enzyme Cascade System into the Metal-Organic Frameworks (MOFs)for the Removal of Microcontaminants and Process Equipment Layout

Babaei, Hassan | 2022

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 56001 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Farhadi, Fatolla; Yaghmaei, Soheila; Ghobadiejad, Zahra
  7. Abstract:
  8. In recent years, the biodegradation of micro-contaminants using enzymes has attracted the attention of many researchers in the field of environment and health. Micro- and nano-scale environments play an important role in enzymatic catalysis that directly affects the activity and stability of enzymes. Therefore, setting a nanoscale environment for enzymes is very important, especially in liquids with undesirable properties (such as pH, temperature, toxicity, etc.) in this work, a method proposed in which the pH and the substrate concentration are regulated for enzymatic catalysis using a metal-organic framework, consisting of Glucose Oxidase (GOx) and Horseradish Peroxidase (HRP) immobilized in ZIF-8. Due to the selective diffusion function of ZIF-8 and in-situ production of gluconic acid and electron donor (hydrogen donor) using GOx, a synergistic effect of selective material diffusion and reaction on the solid-liquid interface was conducted to engineer the microenvironment of HRP to improve its stability and biocatalytic activity. The accuracy of ZIF-8 nanoparticles synthesis and engineering with enzyme immobilization was confirmed using SEM, EDX, TEM, XRD, FT-IR, TGA, CLSM, and nitrogen adsorption-desorption isotherms (BET) analyses. The immobilized enzymes hold a higher thermal, chemical, and storage stability compared to the free enzymes. The result is not only a new method for precise control of the enzymatic reaction but also an intelligent cascade system for the effective removal of micro-contaminants in water in a green process; so that 80% and 58% removal of bisphenol-A and diclofenac micropollutants was achieved using HRP/GOx@ZIF-8 biocatalytic cascade system in 6 hours, respectively
  9. Keywords:
  10. Bisphenol A ; Diclofenac Sodium ; Horseradish Peroxidase (HPR) ; Metal-Organic Framework ; Nanobiocatalyst ; Horseradish Peroxidase ; Enzyme Immobilization

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