Search for: animal-experiment
Total 53 records
کلیدواژه های تکراریCurcumin loading potentiates the neuroprotective efficacy of Fe3O4 magnetic nanoparticles in cerebellum cells of schizophrenic rats, Article Biomedicine and Pharmacotherapy ; Volume 108 , 2018 , Pages 1244-1252 ; 07533322 (ISSN) ; Ashrafi Hafez, A ; Abdorahim, M ; Abdollahifar, M. A ; Shabani, R ; Peirovi, H ; Simchi, A ; Ashtari, K ; Sharif University of Technology
Background: The aim of this study was to investigate the neurotoxic effects of Fe3O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model. Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation,...
Heart Motion Measurement and Prediction for Robotic Assisted Beating Heart Surgery, Ph.D. Dissertation Sharif University of Technology ; Farahmand, Farzam ; Vossoughi, Gholamreza
An essential requirement for performing robotic assisted surgery on a freely beating heart is a prediction algorithm which can estimate the future heart trajectory with a high accuracy in a long horizon. The main objective of this research was measurement and prediction of the heart motion for robotic assisted beating heart surgery. In this study, first the feasibility of a stereo infrared tracking system for measuring the free beating heart motion was investigated by experiments on a heart motion simulator. Simulator experiments revealed a high tracking accuracy when the capturing times were synchronized and the tracker pointed at the target from an appropriate distance.Then, the heart...
Type V collagen in scar tissue regulates the size of scar after heart injury, Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
Cell Press 2020
Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces...
The role of hippo signaling pathway in physiological cardiac hypertrophy, Article BioImpacts ; Volume 10, Issue 4 , 2020 , Pages 251-257 ; Tabrizi, A ; Sharif University of Technology
Tabriz University of Medical Sciences 2020
Introduction: The role of Hippo signaling pathway, which was identified by genetic studies as a key regulator for tissue growth and organ size, in promoting physiological cardiac hypertrophy has not been investigated. Methods: Fourteen male Wistar rats were randomly assigned to the exercise and control groups. The exercise group ran 1 hour per day, 5 days/week, at about 65%-75% VO2max on the motor-driven treadmill with 15ºslope, and the control group ran 15 min/d, 2 days/ week at 9 m/min (0ºinclination), throughout the eight-week experimental period. Forty-eight hours after the last session, hearts were dissected and left ventricles were weighed and stored for subsequent RT-PCR analysis....
The olfactory bulb modulates entorhinal cortex oscillations during spatial working memory, Article Journal of Physiological Sciences ; Volume 71, Issue 1 , 2021 ; 18806546 (ISSN) ; Tabasi, F ; Nazari, M ; Ghazvineh, S ; Salimi, A ; Jamaati, H ; Raoufy, M. R ; Sharif University of Technology
BioMed Central Ltd 2021
Cognitive functions such as working memory require integrated activity among different brain regions. Notably, entorhinal cortex (EC) activity is associated with the successful working memory task. Olfactory bulb (OB) oscillations are known as rhythms that modulate rhythmic activity in widespread brain regions during cognitive tasks. Since the OB is structurally connected to the EC, we hypothesized that OB could modulate EC activity during working memory performance. Herein, we explored OB–EC functional connectivity during spatial working memory performance by simultaneous recording local field potentials when rats performed a Y-maze task. Our results showed that the coherence of delta,...
The effect of temperature on eccentric contraction-induced isometric force loss in isolated perfused rat medial gastrocnemius muscle, Article Tehran University Medical Journal ; Volume 66, Issue 6 , 2008 , Pages 388-395 ; 16831764 (ISSN) ; Keshavarz, M ; Gharibzadeh, S ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, E ; Sharif University of Technology
Tehran University of Medical Sciences 2008
Background: The typical features of eccentric exercise-induced muscle damage are delayed-onset muscle soreness (DOMS) and prolonged loss of muscle strength. It has been shown that passive warmth is effective in reducing muscle injury. Due to the interaction of different systems in vivo, we used isolated perfused medial gastrocnemius skeletal muscle to study the direct effect of temperature on the eccentric contraction-induced force loss. Methods: After femoral artery cannulation of a rat, the left medial gastrocnemius muscle was separated and then the entire lower limb was transferred into a prewarmed (35oC) chamber. With the chamber temperature at 31, 35 and 39oC before and during eccentric...
The effect of poly(ethylene glycol) coating on colloidal stability of superparamagnetic iron oxide nanoparticles as potential MRI contrast agent, Article International Journal of Pharmaceutics ; Volume 433, Issue 1-2 , 2012 , Pages 129-141 ; 03785173 (ISSN) ; Madaah Hosseini, H. R ; Shokrgozar, M. A ; Ahmadi, R ; Oghabian, M. A ; Sharif University of Technology
Superparamganetic iron oxide-based contrast agents in magnetic resonance imaging (MRI) have offered new possibility for early detection of lymph nodes and their metastases. According to important role of nanoparticle size in biodistribution, magnetite nanoparticles coated with different polyethylene glycol (PEG) concentrations up to 10/1 PEG/iron oxide weight ratio in an ex situ manner. To predict the PEG-coated nanoparticle behavior in biological media, such as blood stream or tissue, colloidal stability evaluation was performed to estimate the coating endurance in different conditions. Accordingly, optical absorbance measurements were conducted in solutions with different values of pH and...
Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models, Article Journal of Materials Science: Materials in Medicine ; Volume 28, Issue 5 , 2017 , 73 ; 09574530 (ISSN) ; Eslahi, N ; Mehdipour, A ; Mohammadi, M ; Akbari, M ; Samadikuchaksaraei, A ; Simchi, A ; Sharif University of Technology
Springer New York LLC 2017
Abstract: In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats. Nano-fibrous chitosan-based skin grafts crosslinked by Genepin with physico-mechanical properties close to natural skins were...
Synthesis of a novel magnetic starch-alginic acid-based biomaterial for drug delivery, Article Carbohydrate Research ; Volume 487 , 2020 ; Forouzandehdel, S ; Rezghi Rami, M ; Sharif University of Technology
Elsevier Ltd 2020
The magnetic composite hydrogel was fabricated by the graft copolymerization of itaconic acid (IA) onto starch and Alginic acid in the presence graphene sheets (Gr) and Fe3O4 nanoparticles (Fe3O4@Gr-IA/St-Alg) for Guaifenesin (GFN) delivery and wound healing. The Fe3O4@Gr-IA/St-Alg biomaterial is a hydrogel network endowed the material with magnetic property. In addition, GFN not only achieved effectively bound to the magnetic hydrogel, but also released in a controlled manner. The using external magnetic field has significantly positive influence on the drug release rate. To close, these hydrogel drug carriers offer a favorable platform for magnetically targeted drug delivery as well as a...
Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade, Article Cancer Cell International ; Volume 20, Issue 1 , August , 2020 ; Ebrahimzadeh, M. S ; Abdolalipour, E ; Yazdi, M ; Hosseini Ravandi, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd 2020
Background: Newcastle disease virus (NDV) has shown noticeable oncolytic properties, especially against cervical cancer. However, in order to improve the spread rate and oncotoxicity of the virus, employment of other therapeutic reagents would be helpful. It has been shown that some viral fusogenic membrane glycoproteins (FMGs) could facilitate viral propagation and increase the infection rate of tumor cells by oncolytic viruses. Additionally, immune checkpoint blockade has widely been investigated for its anti-tumor effects against several types of cancers. Here, we investigated for the first time whether the incorporation of influenza hemagglutinin-2 (HA2) FMG could improve the oncolytic...
Stimulus-specific adaptation decreases the coupling of spikes to LFP phase, Article Frontiers in Neural Circuits ; Volume 13 , 2019 ; 16625110 (ISSN) ; Zarei, M ; Jahed, M ; Daliri, M. R ; Sharif University of Technology
Frontiers Media S.A 2019
Stimulus repetition suppresses the neural activity in different sensory areas of the brain. This mechanism of so-called stimulus-specific adaptation (SSA) has been observed in both spiking activity and local field potential (LFP) responses. However, much remains to be known about the effect of SSA on the spike–LFP relation. In this study, we approached this issue by investigating the spike-phase coupling (SPC) in control and adapting paradigms. For the control paradigm, pure tones were presented in a random unbiased sequence. In the adapting paradigm, the same stimuli were presented in a random pattern but it was biased to an adapter stimulus. In fact, the adapter occupied 80% of the...
Role of endurance training in preventing pathological hypertrophy via large tumor suppressor (LATS) changes, Article Iranian Heart Journal ; Volume 20, Issue 3 , 2019 , Pages 52-59 ; 17357306 (ISSN) ; Soori, R ; Choobineh, S ; Gholipour, M ; Sharif University of Technology
Iranian Heart Association 2019
Background: One of the negative effects of cardiac sympathetic hyperactivity is pathologic hypertrophy. Recent studies have indicated that large tumor suppressor (LATS) is one of the molecules which play a critical role in cardiomyocyte apoptosis. Considering the preventive role of exercise training, we evaluated the effects of endurance training on LATS gene expression and its upstream pathway in the present study. Methods: Eighteen male Wistar rats were randomly divided into 2 groups: Endurance and control. Endurance training was performed for 8 weeks, 1 hour per day, and 6 days per week on the treadmill at a 15° inclination. Pathologic hypertrophy was induced with the injection of 3...
Rhythmic air-puff into nasal cavity modulates activity across multiple brain areas: A non-invasive brain stimulation method to reduce ventilator-induced memory impairment, Article Respiratory Physiology and Neurobiology ; Volume 287 , 2021 ; 15699048 (ISSN) ; Salimi, M ; Nazari, M ; Garousi, M ; Tabasi, F ; Dehdar, K ; Salimi, A ; Jamaati, H ; Mirnajafi Zadeh, J ; Arabzadeh, E ; Raoufy, M. R ; Sharif University of Technology
Elsevier B.V 2021
Mechanical ventilation (MV) can result in long-term brain impairments that are resistant to treatment. The mechanisms underlying MV-induced brain function impairment remain unclear. Since nasal airflow modulates brain activity, here we evaluated whether reinstating airflow during MV could influence the memory performance of rats after recovery. Rats were allocated into two study groups: one group received rhythmic air-puff into the nasal cavity during MV and a control group that underwent ventilation without air-puff. During MV, air-puffs induced time-locked event potentials in OB, mPFC and vHPC and significantly increased the oscillatory activity at the air-puff frequency. Furthermore, in...
Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system, Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood...
Preparation and evaluation of bioactive and compatible starch based superabsorbent for oral drug delivery systems, Article Journal of Drug Delivery Science and Technology ; Volume 23, Issue 5 , 2013 , Pages 511-517 ; 17732247 (ISSN) ; Ebrahimi, A. A ; Barzegar, S ; Sharif University of Technology
Novel types of highly swelling hydrogels (superabsorbent) were prepared by grafting crosslinked poly acrylic acid-co-2-hydroxyethylmetacrylate (PAA-co-HEMA) chains onto starch through a free radical polymerization method. The effect of grafting variables (i.e., concentration of methylenebisacrylamide (MBA), acrylic acid/2-hydroxy methymetacrylate (AA/HEMA) weight ratio, ammonium persulfate (APS), starch, neutralization percent, were systematically optimized to achieve a hydrogel with a maximum swelling capacity. The superabsorbent (SAP) formation was confirmed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The controlled-release behavior of...
Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats, Article Journal of Radioanalytical and Nuclear Chemistry ; Volume 287, Issue 1 , January , 2011 , Pages 119-127 ; 02365731 (ISSN) ; Hosseini Salekdeh, S. L ; Mahmoudi, M ; Yousefnia, H ; Majdabadi, A ; Pouladian, M ; Sharif University of Technology
In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O 4) was characterized by FT-IR. The particle size was shown to be ≈5-10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling...
Overexpression of protein kinase Mζ in the hippocampus mitigates alzheimer's disease-related cognitive deficit in rats, Article Brain Research Bulletin ; Volume 166 , 2021 , Pages 64-72 ; 03619230 (ISSN) ; Roosta Azad, R ; Motamedi, F ; Mirzapour Delavar, H ; Ghasemi, S ; Aliakbari, S ; Pourbadie, H. G ; Sharif University of Technology
Elsevier Inc 2021
Accumulation of amyloid beta (Aβ) soluble forms in the cerebral parenchyma is the mainstream concept underlying memory deficit in the early phase of Alzheimer's disease (AD). PKMζ plays a critical role in the maintenance of long-term memory. Yet, the role of this brain-specific enzyme has not been addressed in AD. We examined the impact of hippocampal PKMζ overexpression on AD-related memory impairment in rats. Oligomeric form of Aβ (oAβ) or vehicle was bilaterally microinjected into the dorsal hippocampus of male Wistar rats under stereotaxic surgery. One week later, 2 μl of lentiviral vector (108 T.U. / ml.) encoding PKMζ genome was microinjected into the dorsal hippocampus. Seven days...
Online analysis of local field potentials for seizure detection in freely moving rats, Article Iranian Journal of Basic Medical Sciences ; Volume 23, Issue 2 , 2020 , Pages 173-177 ; Nazari, M ; Shojaei, A ; Raoufy, M. R ; Mirnajafi Zadeh, J ; Sharif University of Technology
Mashhad University of Medical Sciences 2020
Objective(s): Seizure detection during online recording of electrophysiological parameters is very important in epileptic patients. In the present study, online analysis of field potential recordings was used for detecting spontaneous seizures in epileptic animals. Materials and Methods: Epilepsy was induced in rats by pilocarpine injection. During the chronic period of the pilocarpine model, local field potential (LFP) recording was run for at least 24 hr. At the same time, video monitoring of the animals was done to determine the real time of seizure occurrence. Both power and sample entropy of LFP were used for online analysis. Results: Obtained results showed that changes in LFP power...
Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment, Article Virology Journal ; Volume 17, Issue 1 , 2020 ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
BioMed Central Ltd 2020
Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune...
New proline, alanine, serine repeat sequence for pharmacokinetic enhancement of anti-vegf single-domain antibody, Article Journal of Pharmacology and Experimental Therapeutics ; Volume 375, Issue 1 , July , 2020 , Pages 69-75 ; Salimian, M ; Mehdizadeh, A ; Khosravy, M. S ; Vafabakhsh, A ; Karami, E ; Cohan, R. A ; Sharif University of Technology
American Society for Pharmacology and Experimental Therapy 2020
Therapeutic fragmented antibodies show a poor pharmacokinetic profile that leads to frequent high-dose administration. In the current study, for the first time, a novel proline, alanine, serine (PAS) repeat sequence called PAS#208 was designed to extend the plasma half-life of a nanosized anti-vascular endothelial growth factor-A single-domain antibody. Polyacrylamide gel electrophoresis, circular dichroism, dynamic light scattering, and electrophoretic light scattering were used to assess the physicochemical properties of the newly designed PAS sequence. The effect of PAS#208 on the biologic activity of a single-domain antibody was studied using an in vitro proliferation assay. The...