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    کلیدواژه های تکراریCurcumin loading potentiates the neuroprotective efficacy of Fe3O4 magnetic nanoparticles in cerebellum cells of schizophrenic rats

    , Article Biomedicine and Pharmacotherapy ; Volume 108 , 2018 , Pages 1244-1252 ; 07533322 (ISSN) Naserzadeh, P ; Ashrafi Hafez, A ; Abdorahim, M ; Abdollahifar, M. A ; Shabani, R ; Peirovi, H ; Simchi, A ; Ashtari, K ; Sharif University of Technology
    Background: The aim of this study was to investigate the neurotoxic effects of Fe3O4 magnetic- CurNPs on isolated schizophrenia mitochondria of rats as an in vivo model. Methods: We designed CMN loaded superparamagnetic iron oxide nanoparticles (SPIONs) (Fe3O4 magnetic- CurNPs) to achieve an enhanced therapeutic effect. The physicochemical properties of Fe3O4 magnetic- CurNPs were characterized using X-ray diffraction (XRD), and dynamic laser light scattering (DLS) and zeta potential. Further, to prove Fe3O4 magnetic- CurNPs results in superior therapeutic effects, and also, the mitochondrial membrane potential collapse, mitochondrial complex II activity, reactive oxygen species generation,... 

    Heart Motion Measurement and Prediction for Robotic Assisted Beating Heart Surgery

    , Ph.D. Dissertation Sharif University of Technology Mansouri, Saeed (Author) ; Farahmand, Farzam (Supervisor) ; Vossoughi, Gholamreza (Supervisor)
    An essential requirement for performing robotic assisted surgery on a freely beating heart is a prediction algorithm which can estimate the future heart trajectory with a high accuracy in a long horizon. The main objective of this research was measurement and prediction of the heart motion for robotic assisted beating heart surgery. In this study, first the feasibility of a stereo infrared tracking system for measuring the free beating heart motion was investigated by experiments on a heart motion simulator. Simulator experiments revealed a high tracking accuracy when the capturing times were synchronized and the tracker pointed at the target from an appropriate distance.Then, the heart... 

    Type V collagen in scar tissue regulates the size of scar after heart injury

    , Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 Yokota, T ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
    Cell Press  2020
    Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces... 

    The role of hippo signaling pathway in physiological cardiac hypertrophy

    , Article BioImpacts ; Volume 10, Issue 4 , 2020 , Pages 251-257 Gholipour, M ; Tabrizi, A ; Sharif University of Technology
    Tabriz University of Medical Sciences  2020
    Introduction: The role of Hippo signaling pathway, which was identified by genetic studies as a key regulator for tissue growth and organ size, in promoting physiological cardiac hypertrophy has not been investigated. Methods: Fourteen male Wistar rats were randomly assigned to the exercise and control groups. The exercise group ran 1 hour per day, 5 days/week, at about 65%-75% VO2max on the motor-driven treadmill with 15ºslope, and the control group ran 15 min/d, 2 days/ week at 9 m/min (0ºinclination), throughout the eight-week experimental period. Forty-eight hours after the last session, hearts were dissected and left ventricles were weighed and stored for subsequent RT-PCR analysis.... 

    The olfactory bulb modulates entorhinal cortex oscillations during spatial working memory

    , Article Journal of Physiological Sciences ; Volume 71, Issue 1 , 2021 ; 18806546 (ISSN) Salimi, M ; Tabasi, F ; Nazari, M ; Ghazvineh, S ; Salimi, A ; Jamaati, H ; Raoufy, M. R ; Sharif University of Technology
    BioMed Central Ltd  2021
    Cognitive functions such as working memory require integrated activity among different brain regions. Notably, entorhinal cortex (EC) activity is associated with the successful working memory task. Olfactory bulb (OB) oscillations are known as rhythms that modulate rhythmic activity in widespread brain regions during cognitive tasks. Since the OB is structurally connected to the EC, we hypothesized that OB could modulate EC activity during working memory performance. Herein, we explored OB–EC functional connectivity during spatial working memory performance by simultaneous recording local field potentials when rats performed a Y-maze task. Our results showed that the coherence of delta,... 

    The effect of temperature on eccentric contraction-induced isometric force loss in isolated perfused rat medial gastrocnemius muscle

    , Article Tehran University Medical Journal ; Volume 66, Issue 6 , 2008 , Pages 388-395 ; 16831764 (ISSN) Vasaghi Gharamaleki, B ; Keshavarz, M ; Gharibzadeh, S ; Marvi, H ; Mosayebnejad, J ; Ebrahimi Takamjani, E ; Sharif University of Technology
    Tehran University of Medical Sciences  2008
    Background: The typical features of eccentric exercise-induced muscle damage are delayed-onset muscle soreness (DOMS) and prolonged loss of muscle strength. It has been shown that passive warmth is effective in reducing muscle injury. Due to the interaction of different systems in vivo, we used isolated perfused medial gastrocnemius skeletal muscle to study the direct effect of temperature on the eccentric contraction-induced force loss. Methods: After femoral artery cannulation of a rat, the left medial gastrocnemius muscle was separated and then the entire lower limb was transferred into a prewarmed (35oC) chamber. With the chamber temperature at 31, 35 and 39oC before and during eccentric... 

    The effect of poly(ethylene glycol) coating on colloidal stability of superparamagnetic iron oxide nanoparticles as potential MRI contrast agent

    , Article International Journal of Pharmaceutics ; Volume 433, Issue 1-2 , 2012 , Pages 129-141 ; 03785173 (ISSN) Masoudi, A ; Madaah Hosseini, H. R ; Shokrgozar, M. A ; Ahmadi, R ; Oghabian, M. A ; Sharif University of Technology
    Superparamganetic iron oxide-based contrast agents in magnetic resonance imaging (MRI) have offered new possibility for early detection of lymph nodes and their metastases. According to important role of nanoparticle size in biodistribution, magnetite nanoparticles coated with different polyethylene glycol (PEG) concentrations up to 10/1 PEG/iron oxide weight ratio in an ex situ manner. To predict the PEG-coated nanoparticle behavior in biological media, such as blood stream or tissue, colloidal stability evaluation was performed to estimate the coating endurance in different conditions. Accordingly, optical absorbance measurements were conducted in solutions with different values of pH and... 

    Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models

    , Article Journal of Materials Science: Materials in Medicine ; Volume 28, Issue 5 , 2017 , 73 ; 09574530 (ISSN) Mahmoudi, N ; Eslahi, N ; Mehdipour, A ; Mohammadi, M ; Akbari, M ; Samadikuchaksaraei, A ; Simchi, A ; Sharif University of Technology
    Springer New York LLC  2017
    Abstract: In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats. Nano-fibrous chitosan-based skin grafts crosslinked by Genepin with physico-mechanical properties close to natural skins were... 

    Synthesis of a novel magnetic starch-alginic acid-based biomaterial for drug delivery

    , Article Carbohydrate Research ; Volume 487 , 2020 Forouzandehdel, S ; Forouzandehdel, S ; Rezghi Rami, M ; Sharif University of Technology
    Elsevier Ltd  2020
    The magnetic composite hydrogel was fabricated by the graft copolymerization of itaconic acid (IA) onto starch and Alginic acid in the presence graphene sheets (Gr) and Fe3O4 nanoparticles (Fe3O4@Gr-IA/St-Alg) for Guaifenesin (GFN) delivery and wound healing. The Fe3O4@Gr-IA/St-Alg biomaterial is a hydrogel network endowed the material with magnetic property. In addition, GFN not only achieved effectively bound to the magnetic hydrogel, but also released in a controlled manner. The using external magnetic field has significantly positive influence on the drug release rate. To close, these hydrogel drug carriers offer a favorable platform for magnetically targeted drug delivery as well as a... 

    Synergy between hemagglutinin 2 (HA2) subunit of influenza fusogenic membrane glycoprotein and oncolytic Newcastle disease virus suppressed tumor growth and further enhanced by Immune checkpoint PD-1 blockade

    , Article Cancer Cell International ; Volume 20, Issue 1 , August , 2020 Miri, S. M ; Ebrahimzadeh, M. S ; Abdolalipour, E ; Yazdi, M ; Hosseini Ravandi, H ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Background: Newcastle disease virus (NDV) has shown noticeable oncolytic properties, especially against cervical cancer. However, in order to improve the spread rate and oncotoxicity of the virus, employment of other therapeutic reagents would be helpful. It has been shown that some viral fusogenic membrane glycoproteins (FMGs) could facilitate viral propagation and increase the infection rate of tumor cells by oncolytic viruses. Additionally, immune checkpoint blockade has widely been investigated for its anti-tumor effects against several types of cancers. Here, we investigated for the first time whether the incorporation of influenza hemagglutinin-2 (HA2) FMG could improve the oncolytic... 

    Stimulus-specific adaptation decreases the coupling of spikes to LFP phase

    , Article Frontiers in Neural Circuits ; Volume 13 , 2019 ; 16625110 (ISSN) Parto Dezfouli, M ; Zarei, M ; Jahed, M ; Daliri, M. R ; Sharif University of Technology
    Frontiers Media S.A  2019
    Stimulus repetition suppresses the neural activity in different sensory areas of the brain. This mechanism of so-called stimulus-specific adaptation (SSA) has been observed in both spiking activity and local field potential (LFP) responses. However, much remains to be known about the effect of SSA on the spike–LFP relation. In this study, we approached this issue by investigating the spike-phase coupling (SPC) in control and adapting paradigms. For the control paradigm, pure tones were presented in a random unbiased sequence. In the adapting paradigm, the same stimuli were presented in a random pattern but it was biased to an adapter stimulus. In fact, the adapter occupied 80% of the... 

    Simvastatin-loaded nano-niosomes confer cardioprotection against myocardial ischemia/reperfusion injury

    , Article Drug Delivery and Translational Research ; Volume 12, Issue 6 , 2022 , Pages 1423-1432 ; 2190393X (ISSN) Naseroleslami, M ; Mousavi Niri , N ; Akbarzade, I ; Sharifi, M ; Aboutaleb, N ; Sharif University of Technology
    Springer  2022
    Although simvastatin (SIM) has been proven to be a powerful agent against myocardial ischemia/reperfusion (MI/R) injury, poor water solubility, short half-life, and low bioavailability have made it futile while using conventional drug delivery system. Hence, this study aims to investigate therapeutic efficacy of SIM-loaded nano-niosomes on MI/R injury. Surface active agent film hydration method was used to synthesize nano-niosomes. The physicochemical properties of nano-niosomes were characterized using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Moreover, niosomes were characterized in entrapment efficiency (EE) and releasing pattern. Male Wistar rats were... 

    Role of endurance training in preventing pathological hypertrophy via large tumor suppressor (LATS) changes

    , Article Iranian Heart Journal ; Volume 20, Issue 3 , 2019 , Pages 52-59 ; 17357306 (ISSN) Tabrizi, A ; Soori, R ; Choobineh, S ; Gholipour, M ; Sharif University of Technology
    Iranian Heart Association  2019
    Background: One of the negative effects of cardiac sympathetic hyperactivity is pathologic hypertrophy. Recent studies have indicated that large tumor suppressor (LATS) is one of the molecules which play a critical role in cardiomyocyte apoptosis. Considering the preventive role of exercise training, we evaluated the effects of endurance training on LATS gene expression and its upstream pathway in the present study. Methods: Eighteen male Wistar rats were randomly divided into 2 groups: Endurance and control. Endurance training was performed for 8 weeks, 1 hour per day, and 6 days per week on the treadmill at a 15° inclination. Pathologic hypertrophy was induced with the injection of 3... 

    Rhythmic air-puff into nasal cavity modulates activity across multiple brain areas: A non-invasive brain stimulation method to reduce ventilator-induced memory impairment

    , Article Respiratory Physiology and Neurobiology ; Volume 287 , 2021 ; 15699048 (ISSN) Ghazvineh, S ; Salimi, M ; Nazari, M ; Garousi, M ; Tabasi, F ; Dehdar, K ; Salimi, A ; Jamaati, H ; Mirnajafi Zadeh, J ; Arabzadeh, E ; Raoufy, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Mechanical ventilation (MV) can result in long-term brain impairments that are resistant to treatment. The mechanisms underlying MV-induced brain function impairment remain unclear. Since nasal airflow modulates brain activity, here we evaluated whether reinstating airflow during MV could influence the memory performance of rats after recovery. Rats were allocated into two study groups: one group received rhythmic air-puff into the nasal cavity during MV and a control group that underwent ventilation without air-puff. During MV, air-puffs induced time-locked event potentials in OB, mPFC and vHPC and significantly increased the oscillatory activity at the air-puff frequency. Furthermore, in... 

    Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system

    , Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) Zhang, X ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
    An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood... 

    Preparation and evaluation of bioactive and compatible starch based superabsorbent for oral drug delivery systems

    , Article Journal of Drug Delivery Science and Technology ; Volume 23, Issue 5 , 2013 , Pages 511-517 ; 17732247 (ISSN) Pourjavadi, A ; Ebrahimi, A. A ; Barzegar, S ; Sharif University of Technology
    Novel types of highly swelling hydrogels (superabsorbent) were prepared by grafting crosslinked poly acrylic acid-co-2-hydroxyethylmetacrylate (PAA-co-HEMA) chains onto starch through a free radical polymerization method. The effect of grafting variables (i.e., concentration of methylenebisacrylamide (MBA), acrylic acid/2-hydroxy methymetacrylate (AA/HEMA) weight ratio, ammonium persulfate (APS), starch, neutralization percent, were systematically optimized to achieve a hydrogel with a maximum swelling capacity. The superabsorbent (SAP) formation was confirmed by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The controlled-release behavior of... 

    Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats

    , Article Journal of Radioanalytical and Nuclear Chemistry ; Volume 287, Issue 1 , January , 2011 , Pages 119-127 ; 02365731 (ISSN) Jalilian, A. R ; Hosseini Salekdeh, S. L ; Mahmoudi, M ; Yousefnia, H ; Majdabadi, A ; Pouladian, M ; Sharif University of Technology
    In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O 4) was characterized by FT-IR. The particle size was shown to be ≈5-10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling... 

    Preclinical evaluation of 188 Re-HYNIC-PSMA as a novel therapeutic agent

    , Article Journal of Radioanalytical and Nuclear Chemistry ; Volume 331, Issue 2 , 2022 , Pages 841-849 ; 02365731 (ISSN) Hadisi, M ; Vosoughi, N ; Yousefnia, H ; Bahrami-Samani, A ; Zolghadri, S ; Vosoughi, S ; Alirezapour, B ; Sharif University of Technology
    Springer Science and Business Media B.V  2022
    In this study, optimized preparation, quality control, cell assessments and biostribution of 188Re-HYNIC-PSMA in normal rats and tumor bearing mice are studied. Optimized conditions for radiolabeling were evaluated and radiochemical purity (> 99%) was investigated using ITLC and HPLC methods. 188Re-HYNIC-PSMA was stable both in PBS (4 °C) and in human serum (37 °C) even after 48 h. The results showed the complex was cleared from the blood very fast via urinary track. This new radiolabeled compound has a high potential to be considered as an agent for the treatment of patients with PSMA expressing tumors however more biological studies are still needed. © 2022, Akadémiai Kiadó, Budapest,... 

    Overexpression of protein kinase Mζ in the hippocampus mitigates alzheimer's disease-related cognitive deficit in rats

    , Article Brain Research Bulletin ; Volume 166 , 2021 , Pages 64-72 ; 03619230 (ISSN) Amini, N ; Roosta Azad, R ; Motamedi, F ; Mirzapour Delavar, H ; Ghasemi, S ; Aliakbari, S ; Pourbadie, H. G ; Sharif University of Technology
    Elsevier Inc  2021
    Accumulation of amyloid beta (Aβ) soluble forms in the cerebral parenchyma is the mainstream concept underlying memory deficit in the early phase of Alzheimer's disease (AD). PKMζ plays a critical role in the maintenance of long-term memory. Yet, the role of this brain-specific enzyme has not been addressed in AD. We examined the impact of hippocampal PKMζ overexpression on AD-related memory impairment in rats. Oligomeric form of Aβ (oAβ) or vehicle was bilaterally microinjected into the dorsal hippocampus of male Wistar rats under stereotaxic surgery. One week later, 2 μl of lentiviral vector (108 T.U. / ml.) encoding PKMζ genome was microinjected into the dorsal hippocampus. Seven days... 

    Oral administration of lithium chloride ameliorate spinal cord injury-induced hyperalgesia in male rats

    , Article PharmaNutrition ; Volume 21 , 2022 ; 22134344 (ISSN) Rahimi, G ; Mirsadeghi, S ; Rahmani, S ; Izadi, A ; Ghodsi, Z ; Ghodsi, S. M ; Rahimi Movaghar, V ; Kiani, S ; Sharif University of Technology
    Elsevier B.V  2022
    Background: Numerous studies have described the neuroprotective effect of lithium in spinal cord injury in addition to its ameliorative impact on pain sensation. In the present study, we aim to examine the efficacy of 85 mg/kg as well as 50 mg/kg dosage of the lithium chloride (LiCl) through oral consumption in spinal cord injured rats and their effect on gene expression of three candidate genes, corresponding to the hyper-sensitization. Methods: Adult Wistar (male) rats were divided into four experimental groups: control; oral administration of LiCl with 85 mg/kg and 50 mg/kg dosage; and 10 % sucrose receiver as the vehicle. BBB and heat plantar tests were performed weekly throughout four...