Search for: wound-healing
Total 33 records
Modeling and Control of Dermal Wound Healing-Remodeling Phase by Computational Intelligent Techniqes, M.Sc. Thesis Sharif University of Technology ; Seifipour, Navid
Wound healing is a complex biological process dependent on multiple variables: tissue oxygenation, wound size, contamination, etc. Many of these factors depend on multiple factors themselves. Mechanisms for some interactions between these factors are still unknown. In this resarch we try to simulate and control wound healing process with focusing on remodeling phase by neural networks as an intelligence technique. For these purposes some materials like mathematical modeling, finite elements method, and effect of external forces on the scar tissue are used here
Fabrication of Artificial skin with Electrospun of Nanofiber made of PCL and PVA, M.Sc. Thesis Sharif University of Technology ; Shamloo, Amir ; Naghdabadi, Reza ; Vosooghi, Manoochehr ; Firoozbakhsh, Keikhosro
The main goal of this project is to fabricate the scaffolds including Polycaprolactone and Polyvinyle alcohol nanofibers in order to use as artificial skin. For this purpose, electrospinning parameters have been optimized for various injection rates and the scaffolds containing different percent of PCL and PVA have been made. By investigating the cell growth on these scaffolds, the one made up PVA/PCL (30% ) has been introduced as the best scaffold. To improve biological properties of chosen scaffold, a layer of collagen was coated. The angiogenesis capacity has been improved by adding Heparin to PVA nanofibers. Based on assay of heparin release, it was shown that Heparin is released within...
Fabrication of a Multi-Layered Scaffold to Be Used in Dermal Wound Healing, M.Sc. Thesis Sharif University of Technology ; Shamloo, Amir ; Asghari, Mohsen
Wound healing by engineered scaffolds is a new step in bio-technology and medical studies in recent years. The goal of the current study is to propose a novel structure for a tissue-engineered scaffold to be used in wound healing. Influenced from the multi-layered structure of natural human skin, the fabricated scaffold consists of two layers to maximize similarity with natural skin. This product is comprised of an electrospun layer made of polycaprolactone and polyvinyl alcohol and a hydrogel layer made of chitosan and gelatin. In order to form a porous medium in the hydrogel layer, freeze-gelation was used instead of freeze drying. The evaluation of fabricated scaffolds was performed by...
Fabrication of Porous Gelatin-based Scaffold and Evaluation of Effect of Gamma Irradiation, M.Sc. Thesis Sharif University of Technology ; Forounchi, Massoud ; Dadbin, Susan
Biocompatible porous polymeric scaffolds provide a suitable 3-D environment for proliferation of stem cells in human body. For instance, growth and proliferation concurrent with differentiation of stem cells in such scaffolds can regenerate the tissues or organs. In this attitude we fabricated a porous Gelatin-based scaffold using both of freeze-drying and freeze-extraction methods.Also, effect fo gamma irradiation on microstructure of scaffolds was investigated. In addition, poly(ethylene glycol) was employed to make the scaffold softer. Moreover, effects of various parameters including freezing temperature, cross-linking agent concentration, gelatin and PEG concentrations and their...
Construction of Scaffold by Electrospinning Method for Use in Skin Tissue Engineering, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran ; Vosoughi, Manouchehr
Making artificial skin and skin alternatives is one of the most important areas in tissue engineering. Although much progress has been made so far, there is still no definitive cure for second- and third-degree burns. To create new tissue in the body, a suitable substrate for cells is needed, which is called scaffolding, and an ideal scaffold in tissue engineering should mimic the dimensions of extracellular matrix, and nanofibers seem to be the best option for this purpose. Among the methods of manufacturing of nanofibers, electrospun is very easy and accurate method. In previous studies, many natural and synthetic polymers such as chitosan, alginate, collagen, polyathylene oxide, etc....
Fabrication and Chracatization of Novel Electrospun Chitosan Fibers Reinforced with Bacterial Cellulose and Nanodiamond for Wound Dressing Applications, M.Sc. Thesis Sharif University of Technology ; Simchi, Abdolreza
Chronic wounds are among the serious injuries which have called the attention of scientists to invest in an effort to fabricate temporary skin grafts and wound dressing materials. One of the widely acceptable solutions to this obstacle is to design a bioactive scaffold capable of regenerating tissue as well as delivering active agents to the site specific area. To this end, natural biopolymers are considered as suitable candidates to be employed in regenerative medicine due to their excellent biocompatibility. In addition, nanoparticles possess special properties such as the ability in sequestering the drug release which make them promising candidates in the fabrication of the...
Type V collagen in scar tissue regulates the size of scar after heart injury, Article Cell ; Volume 182, Issue 3 , 2020 , Pages 545-562.e23 ; McCourt, J ; Ma, F ; Ren, S ; Li, S ; Kim, T. H ; Kurmangaliyev, Y. Z ; Nasiri, R ; Ahadian, S ; Nguyen, T ; Tan, X. H. M ; Zhou, Y ; Wu, R ; Rodriguez, A ; Cohn, W ; Wang, Y ; Whitelegge, J ; Ryazantsev, S ; Khademhosseini, A ; Teitell, M. A ; Chiou, P. Y ; Birk, D. E ; Rowat, A. C ; Crosbie, R. H ; Pellegrini, M ; Seldin, M ; Lusis, A. J ; Deb, A ; Sharif University of Technology
Cell Press 2020
Scar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function. A systems genetics approach across 100 in-bred strains of mice demonstrated that collagen V is a critical driver of postinjury heart function. We show that collagen V deficiency alters the mechanical properties of scar tissue, and altered reciprocal feedback between matrix and cells induces...
Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models, Article Journal of Materials Science: Materials in Medicine ; Volume 28, Issue 5 , 2017 , 73 ; 09574530 (ISSN) ; Eslahi, N ; Mehdipour, A ; Mohammadi, M ; Akbari, M ; Samadikuchaksaraei, A ; Simchi, A ; Sharif University of Technology
Springer New York LLC 2017
Abstract: In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats. Nano-fibrous chitosan-based skin grafts crosslinked by Genepin with physico-mechanical properties close to natural skins were...
Synthesis of nanobentonite–poly(vinyl alcohol)–bacterial cellulose nanocomposite by electrospinning for wound healing applications, Article Physica Status Solidi (A) Applications and Materials Science ; Volume 217, Issue 6 , 2020 ; Ghanbari, H ; Mahmoudi, N ; Haramshahi, S. M. A ; Javadpour, J ; Sharif University of Technology
Wiley-VCH Verlag 2020
Polymer-based composites are used for wound healing applications. This work aims to prepare an inorganic-polymer nanocomposite based on bentonite, poly(vinyl alcohol), and bacterial cellulose by electrospinning for wound healing. The nanocomposite is synthesized using a solution intercalation technique, with 1–2 wt% nanobentonite concentration variation. The effects of commercial and laboratory-synthesized nanobentonite as well as the extract of the green walnut shell (EGWS) are examined and characterized by different techniques. The addition of nanobentonite increases the average size of fibers and tensile strength up to 200 nm and more than 15 MPa, respectively, due to the presence of...
Synthesis of a novel magnetic starch-alginic acid-based biomaterial for drug delivery, Article Carbohydrate Research ; Volume 487 , 2020 ; Forouzandehdel, S ; Rezghi Rami, M ; Sharif University of Technology
Elsevier Ltd 2020
The magnetic composite hydrogel was fabricated by the graft copolymerization of itaconic acid (IA) onto starch and Alginic acid in the presence graphene sheets (Gr) and Fe3O4 nanoparticles (Fe3O4@Gr-IA/St-Alg) for Guaifenesin (GFN) delivery and wound healing. The Fe3O4@Gr-IA/St-Alg biomaterial is a hydrogel network endowed the material with magnetic property. In addition, GFN not only achieved effectively bound to the magnetic hydrogel, but also released in a controlled manner. The using external magnetic field has significantly positive influence on the drug release rate. To close, these hydrogel drug carriers offer a favorable platform for magnetically targeted drug delivery as well as a...
Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing, Article Advanced Drug Delivery Reviews ; Volume 123 , 2018 , Pages 33-64 ; 0169409X (ISSN) ; Sahandi Zangabad, P ; Moosavi Basri, S. M ; Sahandi Zangabad, K ; Ghamarypour, A ; Aref, A. R ; Karimi, M ; Hamblin, M. R ; Sharif University of Technology
Elsevier B.V 2018
According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally...
Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing, Article International Journal of Biological Macromolecules ; Volume 134 , 2019 , Pages 280-289 ; 01418130 (ISSN) ; Tamjid, E ; Eslahi, N ; Simchi, A ; Sharif University of Technology
Elsevier B.V 2019
To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique. The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG). FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry. The hydrogel particles were uniformly...
Modeling of forced dermal wound healing using intelligent techniques, Article 2010 The 2nd International Conference on Computer and Automation Engineering, ICCAE 2010, 26 February 2010 through 28 February 2010, Singapore ; Volume 2 , 2010 , Pages 207-211 ; 9781424455850 (ISBN) ; Ghaemi Osgouie, K ; Sharif University of Technology
Wound healing is a complex biological process dependent on multiple variables: tissue oxygenation, wound size, contamination, etc. Many of these factors depend on multiple factors themselves. Mechanisms for some interactions between these factors are still unknown but it is generally accepted that collagen synthesis, accumulation and organization are increased by mechanical stimuli, resulting in a forced healing process which improves mechanical properties of the damaged tissue. In this paper we focus on the neural networks and regard them as function approximators, and attempt to simulate remodeling phase of dermal wound healing process using neural networks as an intelligent technique
Modeling of dermal wound healing-remodeling phase by neural networks, Article 2009 International Association of Computer Science and Information Technology - Spring Conference, IACSIT-SC 2009, Singapore, 17 April 2009 through 20 April 2009 ; 2009 , Pages 447-450 ; 9780769536538 (ISBN) ; Seifipour, N ; Sharif University of Technology
Wound healing is a complex biological process dependent on multiple variables: tissue oxygenation, wound size, contamination, etc. Many of these factors depend on multiple factors themselves. Mechanisms for some interactions between these factors are still unknown, presenting a barrier for scientists intending to model wound healing using an object-based programming approach. In this paper we focus on the neural networks and regard them as function approximators, and attempt to simulate remodeling phase of dermal wound healing process using neural networks as an intelligence technique. © 2009 IEEE
Mathematical modeling of dermal wound healing's remodeling phase: a finite element solution, Article 2009 International Association of Computer Science and Information Technology - Spring Conference, IACSIT-SC 2009, Singapore, 17 April 2009 through 20 April 2009 ; 2009 , Pages 529-532 ; 9780769536538 (ISBN) ; Seifipour, N ; Sharif University of Technology
Though wound healing process is well-researched, this area is poorly known. One reason is that all interactions have not been discovered, the main reason, though, is that the involved processes interact in a very complicated manner with nonlinear feedback. Such complex feedback mechanisms can be easily addressed by mathematical modeling. This paper contains a review of the mathematical modeling of cell interaction with extracellular matrix components during the process of dermal wound healing with focusing on remodeling phase. The models are of partial differential equation type and solved by finite element method. © 2009 IEEE
Mathematical modeling of dermal wound healing: A numerical solution, Article 2010 The 2nd International Conference on Computer and Automation Engineering, ICCAE 2010, 26 February 2010 through 28 February 2010, Singapore ; Volume 2 , 2010 , Pages 153-156 ; 9781424455850 (ISBN) ; Ghaemi Osgouie, K ; Sharif University of Technology
Though wound healing process is well-researched, this area is poorly known. One reason is that all interactions have not been discovered, the main reason, though, is that the involved processes interact in a very complicated manner with nonlinear feedback. Such complex feedback mechanisms can be easily addressed by mathematical modeling. This paper contains a review of the mathematical modeling of cell interaction with extracellular matrix components during the process of dermal wound healing with focusing on remodeling phase. The models are of partial differential equation type and solved by numerical method
Freeze-gelled alginate/gelatin scaffolds for wound healing applications: An in vitro, in vivo study, Article Materials Science and Engineering C ; Volume 113 , 2020 ; Shamloo, A ; Kamali, A ; Sharif University of Technology
Elsevier Ltd 2020
In this study, fabrication of a three-dimensional porous scaffold was performed using freeze gelation method. Recently, fabrication of scaffolds using polymer blends has become common for many tissue engineering applications due to their unique tunable properties. In this work, we fabricated alginate-gelatin porous hydrogels for wound healing application using a new method based on some modifications to the freeze-gelation method. Alginate and gelatin were mixed in three different ratios and the resulting solutions underwent freeze gelation to obtain 3D porous matrices. We analyzed the samples using different characterization tests. The scanning electron microscopy (SEM) results indicated...
Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study, Article International Journal of Pharmaceutics ; Volume 592 , 2021 ; 03785173 (ISSN) ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
Elsevier B.V 2021
In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,...
Fabrication and evaluation of a bilayer hydrogel-electrospinning scaffold prepared by the freeze-gelation method, Article Journal of Biomechanics ; Volume 98 , 2020 ; Shamloo, A ; Sharif University of Technology
Elsevier Ltd 2020
This study presents a bilayer structure as a skin scaffold comprised of an electrospun sheet layer made of polycaprolactone and polyvinil alcohol and a porous hydrogel layer made of chitosan and gelatin. The hydrogel layer was fabricated by employing the freeze-gelation technique. The bilayer structure was achieved by pouring the hydrogel solution on the electrospun sheet at the bottom of a mold followed by the freeze-gelation technique to obtain a porous structure in the hydrogel. The hydrogel and hydrogel-electrospun samples were characterized by scanning electron microscopy, swelling, tensile strength, in vitro and in vivo analyses. From a mechanical strength standpoint, the combination...
Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering, Article Scientific Reports ; Volume 11, Issue 1 , 2021 ; 20452322 (ISSN) ; Shamloo, A ; Bahadoran, M ; Moravvej, H ; Sharif University of Technology
Nature Research 2021
Using the skin tissue engineering approach is a way to help the body to recover its lost skin in cases that the spontaneous healing process is either impossible or inadequate, such as severe wounds or burns. In the present study, chitosan/gelatin-based scaffolds containing 0.25, 0.5, 0.75, and 1% allantoin were created to improve the wounds’ healing process. EDC and NHS were used to cross-link the samples, which were further freeze-dried. Different in-vitro methods were utilized to characterize the specimens, including SEM imaging, PBS absorption and degradation tests, mechanical experiments, allantoin release profile assessment, antibacterial assay, and cell viability and adhesion tests....