Sharif Digital Repository

This paper presents a comprehensive 3D numerical simulation of reacting flows in micro scale dimension through centrifugal, or Lab-On-a-CD (LOCD), and pressure-driven, or Lab-On-a-Chip (LOC) devices. Three different serpentine channel configurations (rectangular, triangular and sinusoidal) are investigated. In these configurations, two chemical species enter from two inlets and according to an irreversible chemical reaction, start yielding other species. Both Newtonian and generalized Newtonian fluids are considered in the simulations and the results are compared for both LOC and LOCD devices. Besides, the effects of different parameters such as the aspect ratio of channels’ cross section,... 

We study the hydrodynamic dispersion (HD) by electroosmotic flow in soft microchannels. Considering a fully developed flow in a slit microchannel of low surface potential and adopting the Taylor dispersion theory, we derive analytical solutions for the solute concentration field and the effective dispersion coefficient. We also conduct numerical analyses to broaden the paper's scope to high surface potentials and to specify a criterion for the validity of the Debye-Hückel linearization in soft microconduits as well as to investigate the broadening of an analyte band from the time of injection. It is demonstrated that the effective dispersion coefficient of a neutral solute band is generally... 

Microfluidic devices have been widely used for biological and cellular studies. Microbioreactors for three-dimensional (3D) multicellular spheroid culture are now considered as the next generation in in vitro diagnostic tools. The feasibility of using 3D cell aggregates to form multicellular spheroids in a microbioreactor with U-shaped barriers has been demonstrated experimentally. A barrier array is an alternative to commonly used microwell traps. The present study investigates oxygen and glucose concentration distributions as key parameters in a U-shaped array microbioreactor using finite element simulation. The effect of spheroid diameter, inlet concentration and flow rate of the medium... 

This paper presents an analysis of the combined electro-osmotic and pressure-driven axial flows of viscoelastic fluids in a rectangular microchannel with arbitrary aspect ratios. The rheological behavior of the fluid is described by the complete form of Phan-Thien–Tanner (PTT) model with the Gordon–Schowalter convected derivative which covers the upper convected Maxwell, Johnson–Segalman and FENE-P models. Our numerical simulation is based on the computation of 2D Poisson–Boltzmann, Cauchy momentum and PTT constitutive equations. The solution of these governing nonlinear coupled set of equations is obtained by using the second-order central finite difference method in a non-uniform grid... 

Recently, electrode-based Dielectrophoresis (eDEP) has been used for particle manipulation by means of triangular electrodes. In this theoretical and numerical study, a microchannel with quarter-of-ellipse electrodes is presented and a detailed comparison with triangular electrodes is provided. Electric field, resultant DEP force, and particle trajectories for each microchannel are evaluated by means of COMSOL Multiphysics 4.4. Afterwards, focusing and separation efficiencies of the systems are assessed and compared. Finally, separation efficiency of our proposed model for live and dead cells is compared with that of our previous model published in the literature [1]. It is demonstrated that... 

The biofluids being manipulated in lab-on-a-chip devices usually contain elastic macromolecules. Accordingly, for an accurate modeling of the relevant flow physics one should invoke viscoelastic constitutive equations. In this paper, attention is paid toward the hydrodynamic dispersion by the fully developed electroosmotic flow of PTT viscoelastic fluids in slit microchannels of low zeta potential. Adopting the Taylor–Aris approach, analytical solutions are derived for late-time solute concentration and effective dispersion coefficient. Finite element-based numerical simulations are also conducted to monitor the broadening of an analyte band from the moment of injection. Both approaches are... 

An experimental test bed based on single particle tracking techniques is employed in order to investigate the velocity domain, slip velocity, and settling distribution of micro-particles in low-Reynolds number poiseuille flow in converging–diverging microchannel. Three-dimensional velocity domain of particles are studied in the presence of walls and compared with the particle-free fluid. The results show that the velocity of particles moving near the side walls of microchannel decreases about 30 % compared to those moving at the centerline. Furthermore, the effects of converging–diverging geometry on sedimentation of micro-particles are considered. The results show an average decrease of... 

Particle separation has a variety of applications in biology, chemistry and industry. Among them, circulating tumor cells (CTCs) separation has drawn significant attention to itself due to its high impact on both cancer diagnosis and therapeutics. In recent years, there has been growing interest in using inertial microfluidics to separate micro/nano particles based on their sizes. This technique offers label-free, high-throughput and efficient separation and can be easily fabricated. However, further improvements are needed for potential clinical applications. In this study, a novel inertial separation technique using spiral microchannel having stair-like cross section is introduced. The... 

The Lab On a CD (LOCD), also known as Centrifugal Microfluidics, has evolved into a sophisticated platform for performing biomedical assays due to its marvelous miniaturization and accurate simulation of biological reactions. Among the numerous applications of the LOCD is fluid mixing. In this paper a centrifugal, serpentine micromixer is simulated and reformed toward better mixing performance. The micromixer was chosen to be curved as a curved design was found to be thrice as functional and compact as a rectilinear design, mixing-wise. The two angular velocity and opening radius parameters were originally hypothesized to affect mixing performance. Effect of angular velocity was studied over... 

The limitations of the microfabrication technology do not allow producing perfectly smooth microchannels. Hence, exploring the influences of roughness on transport phenomena in microtubes is of great importance to the scientific community. In the present work, consideration is given toward the corrugated roughness effects on fully developed electroosmotic flow and heat transfer in circular microtubes. Analytical solutions based on perturbation technique are presented for the problem assuming a low zeta potential under the constant heat flux boundary condition of the first kind. It is revealed that higher values of the corrugation number and relative roughness give rise to smaller Nusselt... 

An experimental test bed based on single particle tracking techniques is employed in order to investigate the velocity domain, slip velocity, and settling distribution of micro-particles in low-Reynolds number poiseuille flow in converging–diverging microchannel. Three-dimensional velocity domain of particles are studied in the presence of walls and compared with the particle-free fluid. The results show that the velocity of particles moving near the side walls of microchannel decreases about 30 % compared to those moving at the centerline. Furthermore, the effects of converging–diverging geometry on sedimentation of micro-particles are considered. The results show an average decrease of... 

The secondary flow of PTT fluids in rectangular cross-sectional plane of microchannels under combined effects of electroosmotic and pressure driving forces is the subject of the present study. Employing second-order central finite difference method in a very refined grid network, we investigate the effect of electrokinetic and geometric parameters on the pattern, strength and the average of the secondary flow. In this regard, we try to illustrate the deformations of recirculating vortices due to change in the dimensionless Debye–Hückel and zeta potential parameters as well as channel aspect ratio. We demonstrate that, in the presence of thick electric double layers, significant alteration... 

This paper investigates two-dimensional, time-independent elecroosmotic pressuredriven flow generated by a direct current electric potential with asymmetrical and symmetrical zeta potential distributions along the microchannel walls. Fluid flow through the horizontal microchannel is simulated using a numerical method. Two different cases are proposed to study the effect of electric potential on the flow field. First, negative electric potential is applied on the microchannel walls. In this case, large segments with negative electric potential are initially placed on the first half of the microchannel walls with two different arrangements. Afterward, smaller segments with negative electric... 

Some of electrokinetic-based biomicrofluidic devices work at zeta potentials that are sufficiently high for ionic size (steric) effects to show up. In the present effort, consideration is given to the steric effects on the hydrodynamics of electroosmotic flow in a rectangular microchannel. The distinction between this research and the previous ones is that we account for non-linear rheology of the fluids encountered in biomicrofluidic systems by means of the power-law viscosity model. The method of analysis consists of a finite-difference-based numerical procedure for a non-uniform distribution of grid points, which is applied to the dimensionless form of the governing equations including... 

Ammonia detoxification is one of the main functions of the liver results in production of urea. In this study ammonia elimination and urea production was simulated in a microchannel mimicking the hepatic porto central axis. Navier- Stockes equations along with convection equations were solved for the related species in the entire domain. Since the Reynolds number was small (~1) the fluid flow regime was laminar. Urea cycle was modeled regarding its four main enzymes. Twelve rate equations were also solved in order to obtain the concentration of each metabolites participating in urea cycle. Concentration of the urea reached its maximum ca. 1.2e-5 M at the end of the channel which is in good... 

This is a theoretical study dealing with mixed electroosmotic and pressure-driven flow of a Newtonian liquid in a rectangular microchannel. Both and thermal boundary conditions are considered and the Debye-Hückel linearization is invoked. The governing equations are made dimensionless assuming fully developed conditions and then analytically solved using an infinite series solution. The governing factors are found to be the dimensionless Debye-Hückel parameter, velocity scale ratio, dimensionless Joule heating parameter, and channel aspect ratio. The results indicate that the Nusselt number is an increasing function of the channel aspect ratio, whereas the opposite is true for the velocity... 

Dielectrophoresis (DEP) is an electrokinetic phenomenon which is used for manipulating micro- and nanoparticles in micron-sized devices with high sensitivity. In recent years, electrode-based DEP by patterning narrow oblique electrodes in microchannels has been used for particle manipulation. In this theoretic study, a microchannel with triangular electrodes is presented and a detailed comparison with oblique electrodes is made. For each shape, the behavior of particles is compared for three different configurations of applied voltages. Electric field, resultant DEP force, and particle trajectories for configurations are computed by means of Rayan native code. The separation efficiency of... 

Microbubbles are broadly used as ultrasound contrast agents. In this paper we use a low-cost flow focusing microchannel fabrication method for preparing microbubble contrast agents by using some surface active agents and a viscosity enhancing material to obtain appropriate microbubbles with desired lifetime and stability for any in vitro infusion for velocity measurement. All the five parameters that govern the bubble size extract and some efforts are done to achieve the smallest bubbles by adding suitable surfactant concentrations. By using these microbubbles for the echo-particle image velocimetry method, we experimentally determine the velocity field of steady state and pulsatile pipe... 

The present study attempts to analyze the extended Graetz problem in combined electroosmotic and pressure driven flows in rectangular microchannels, by employing a variational formulation. Both the Joule heating and axial conduction effects are taken into consideration. Since assuming a uniform inlet temperature profile is not consistent with the existence of these effects, a step change in wall temperature is considered to represent physically conceivable thermal entrance conditions. The method of analysis considered here is primarily analytical, in which series solutions are presented for the electrical potential, velocity, and temperature. For general treatment of the eigenvalue problem... 

In high Knudsen number flow regimes microgas flow analysis may not be performed accurately using the classical CFD methods. Alternatively, the gas flow through micro-geometries can be investigated reliably using the direct simulation Monte Carlo (DSMC) method. Our concern in this paper is to use DSMC to study the mixing of two gases in entering simultaneously into a microchannel. The mixing process is assumed to be complete when the mass composition of each species deviates by no more than ±1% from its equilibrium composition. To enhance the mixing process, we focus on the effects of inlet-outlet pressure difference and the pressure ratios of the two incoming CO and N2 streams on the mixing...