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Simulation and Optimization of α-amylase Enzyme Purification Process
Using Column Chromatography
,
M.Sc. Thesis
Sharif University of Technology
;
Rosstaazad, Reza
(Supervisor)
Abstract
Enzymes, which are biochemical catalysts, have important applications in different industries such as food, pharmaceutical, medical, chemical identification and etc. Several methods have been used for separation of enzymes among which precipitation, chromatography and two-phase system. In this work, purification of α-amylase has been done by Ammonium Sulfate and Acetone precipitation, gel filtration based on Sephadex, and PEG-salt aqueous two-phase system. Furthermore, mass and momentum conservations’ equations were solved simultaneously to simulate the process based on the finite element method (FEM). Crude enzyme was precipitated with different saturations of ammonium sulfate and the...
A misbehavior‐tolerant multipath routing protocol for wireless Ad hoc networks [electronic resource]
, Article International Journal of Research in Wireless Systems (IJRWS) ; Vol. 2, Issue 9, pp. , Sep. 2013 ; Pakravan, Mohammad Reza ; Aref, Mohammad Reza ; Sharif University of Technology
Abstract
Secure routing is a major key to service maintenance in ad hoc networks. Ad hoc nature exposes the network to several types of node misbehavior or attacks. As a result of the resource limitations in such networks nodes may have a tendency to behave selfishly. Selfish behavior can have drastic impacts on network performance. We have proposed a Misbehavior-Tolerant Multipath Routing protocol (MTMR) which detects and punishes all types of misbehavior such as selfish behavior, wormhole, sinkhole and grey-hole attacks. The protocol utilizes a proactive approach to enforce cooperation. In addition, it uses a novel data redirection method to mitigate the impact of node misbehavior on network...
Phase-field simulation of counter-current spontaneous imbibition in a fractured heterogeneous porous medium
, Article Physics of Fluids ; Volume 29, Issue 6 , 2017 ; 10706631 (ISSN) ; Akhlaghi Amiri, A ; Sharif University of Technology
American Institute of Physics Inc
2017
Abstract
Spontaneous imbibition is well-known to be one of the most effective processes of oil recovery in fractured reservoirs. However, the detailed pore-scale mechanisms of the counter-current imbibition process and the effects of different fluid/rock parameters on this phenomenon have not yet been deeply addressed. Thiswork presents the results of a newpore-level numerical study of counter-current spontaneous imbibition, using coupled Cahn-Hilliard phase field and Navier-Stokes equations, solved by a finite element method. A 2D fractured medium was constructed consisting of a nonhomogeneous porous matrix, in which the grains were represented by an equilateral triangular array of circles with...
Analysis and Improvement of Intrusion Detection Methods in Data Network Routers
, M.Sc. Thesis Sharif University of Technology ; Aref, Mohammad Reza (Supervisor) ; Pakravan, Mohammad Reza (Co-Advisor)
Abstract
High-quality online services demand reliable and fast packet delivery at the network layer. However, clear evidence documents the existence of compromised routers in the ISP and enterprise networks, threatening network availability and reliability. A compromised router can stealthily drop, modify, inject, or delay packets in the forwarding path to launch Denial-of-Service, surveillance, man-in-the-middle attacks, etc. So researches tried to create intrusion detection methods to identify adversarial routers and switches. To this end, data-plane fault localization (FL) aims to identify faulty links and is an effective means of achieving high network availability. FL protocols use...
Topological Defects in Confined Nematics by Planar Anchoring
, M.Sc. Thesis Sharif University of Technology ; Ejtehadi, Mohammad Reza (Supervisor) ; Mozaffari, Mohammad Reza (Supervisor)
Abstract
Confining nematic liquid crystal between two curved boundary conditions while the nematic molecules have a degenerate planar anchoring leads to complex and beautiful textures of molecular disparagement(defect) in the bulk and on the surfaces. Such the liquid crystal shells are made with double-emulsion techniques in microfluidic devices have provided applications for micro-scale colloidal linkers.In this work, we have numerically minimized the elastic energy in one-constant approximation in present of Fournier’s degenerate surface potential with finite element method. The nematic shell is confined between two spherical surfaces that we have studied the final energies and their related...
Pore-level influence of wettability on counter-current spontaneous imbibition
, Article 79th EAGE Conference and Exhibition 20172017 ; 2017 ; 9789462822177 (ISBN) ; Akhlaghi Amiri, H. A ; Sharif University of Technology
2017
Abstract
Medium wettability plays the main role during spontaneous imbibition (SI) in fractured porous media; however, one can rarely find pore-scale studies addressing wettability effects on this process. In present work Cahn-Hilliard phase field coupled with Navier-Stokes equations were solved using finite element method to simulate counter-current SI in a horizontal heterogeneous porous medium containing fracture. Capillary governed fluid displacement in media with different contact angles - ?=p/10 (extremely water-wet) up to ?=p/2 (neutral wet) - were simulated. The simulated models realistically captured micro-scale mechanisms during imbibition process in strongly water wet media, e.g., oil film...
Pore-level influence of micro-fracture parameters on visco-capillary behavior of two-phase displacements in porous media
, Article Advances in Water Resources ; Volume 113 , 2018 , Pages 260-271 ; 03091708 (ISSN) ; Akhlaghi Amiri, H. A ; Sharif University of Technology
Elsevier Ltd
2018
Abstract
In this work, coupled Cahn–Hilliard phase field and Navier–Stokes equations were solved using finite element method to address the effects of micro-fracture and its characterizations on water-oil displacements in a heterogeneous porous medium. Sensitivity studies at a wide range of viscosity ratios (M) and capillary numbers (Ca), and the resultant log Ca–log M stability phase diagram, revealed that in both media, with/without fracture, the three regimes of viscous fingering, capillary fingering and stable displacement similarly occur. However, presence of the fracture caused water channeling phenomenon which resulted in reduction of the number of active fingers and hence the final oil...
Improvement of Routing Security in Ad Hoc Networks Against Several Attacks
, M.Sc. Thesis Sharif University of Technology ; Aref, Mohammad Reza (Supervisor) ; Pakravan, Mohammad Reza (Supervisor)
Abstract
Secure routing is a major key to service maintenance in wireless ad hoc networks. Ad hoc nature exposes the network to several types of node misbehavior or attacks. Wormhole attack is one of the most severe active attacks to mobile ad hoc networks. Noting the resource limitations in such networks, nodes may have a tendency to behave selfishly. Selfish behavior can have drastic impacts on network performance. We have proposed a Misbehavior-Tolerant Multipath Routing protocol (MTMR) which detects and punishes all types of misbehavior such as selfish behavior, wormhole, sinkhole and grey-hole attacks. The protocol utilizes a proactive approach to enforce cooperation. In addition, it uses a...
Solving Symmetric Nonlinear Equations System Using BFGS Trust Region Quadratic Method
, M.Sc. Thesis Sharif University of Technology ; Razvan, Mohammad Reza (Supervisor) ; Peyghami, Mohammad Reza (Supervisor)Design and Implementation of 2.5 Gbps Circuit Switching Fabric
, M.Sc. Thesis Sharif University of Technology ; Pakravan, Mohammad Reza (Supervisor) ; Movahhedy, Mohammad Reza (Supervisor)
Abstract
Providing high bandwidth network infrastructures for ever increasing need of data transport is of great importance. The underling infrastructure for many communication services such as GSM/3G/4G mobile networks and Internet services is Synchronous Digital Hierarchy (SDH) optical transport systems. SDH are standardized protocols that multiplex multiple lower rate digital bit streams, such as E1 and Ethernet, and transfer them synchronously over optical fiber using lasers or LEDs. In addition to high data transfer rates, flexible network management and protection mechanisms have great importance, hence are part of SDH standards. In order to obtain flexible network architecture and protected...
Preserving Privacy in Smart Grid
, M.Sc. Thesis Sharif University of Technology ; Aref, Mohammad Reza (Supervisor) ; Pakravan, Mohammad Reza (Co-Advisor)
Abstract
Development of Smart Grid and deployment of smart meters in large scale has raised a lot of concerns regarding customers’ privacy. Consequently, several schemes have been proposed to overcome the above mentioned issue. These schemes mainly rely on data aggregation as a method of protecting users’ privacy from the grid operators. However, the main problem with most of these schemes is the fact that they require a large amount of processing power at the meter side. This, together with the fact that smart meters don’t usually have a powerful processor, can cause the unavailability of smart meter data at the required time for operators of the grid, and at the same time disables smart meters from...
Effect of the Geometry of Colloidal Particles on Nematic Liquid Crystals
, Ph.D. Dissertation Sharif University of Technology ; Ejtehadi, Mohammad Reza (Supervisor) ; Mozaffari, Mohammad Reza (Co-Advisor)
Abstract
A liquid crystal is a state of matter composed of highly anisotropic building blocks. When liquid crystals are exposed to surfaces with given shapes and anchoring conditions, the orientation of their molecules gets spatially disordered and topological defects may form in the system. The interplay between liquid crystals and the geometry and topology of the colloidal particles has motivated extensive recent studies.In this thesis, we present the numerical modeling results for two faceted colloidal particles in a thermotropic nematic liquid crystal. To obtain the equilibrium nematic field with a finiteelement method, we numerically minimize the free energy of the system that based on the...
Solving Nonlinear Systems of Mixed Iqualities and Inequalities Via Trust Region Quadratic Methods
, M.Sc. Thesis Sharif University of Technology ; Razvan, Mohammad Reza (Supervisor) ; Payghami, Mohammad Reza (Supervisor)Jamming Resilient Algorithms for Rendezvous in Cognitive Radio Networks
, M.Sc. Thesis Sharif University of Technology ; Aref, Mohammad Reza (Supervisor) ; Pakravan, Mohammad Reza (Co-Advisor)
Abstract
Dynamic spectrum access with the aid of cognitive radio (CR) technology is a viable solution to spectrum scarcity. There are many military and emergency applications that require the use of cognitive radio ad-hoc networks (CRAHNs). In these net- works, nodes must first find each other in a wide spectrum and then exchange their desired data. The process of finding each other in a multichannel environment is a challenging task and is usually known as rendezvous. Since rendezvous is the first step in communication, disrupting will disable the nodes from establishing proper communication sessions. That results in a drastic degrade in the performance of the network. Therefore, it is important to...
Numerical Study of Spray Impingement to Solid Wall
, M.Sc. Thesis Sharif University of Technology ; Morad, Mohammad Reza (Supervisor) ; Jahannama, Mohammad Reza (Co-Supervisor)
Abstract
Spray-wall impingement is one of the phenomenon that associated in different subject such as spray cooling, combustion chamber and gas turbine. With the impingement of spray to the wall, liquid film begins to form. Wall film characteristics is still an important subject to study. Due to the various stages of liquid film formation from injection of spray till spray-wall impingement, the investigation of this phenomenon is far more complicated. This paper is meant to provide detailed data on spray-wall impingement and wall film formation physics with the help of computational analysis of OpenFOAM . A Lagrangian-Eulerian method based on discrete phase model (DPM) was employed to model...
Assessing and Improving Algorithms to Control the Propagation of Light through Scattering Media
, M.Sc. Thesis Sharif University of Technology ; Rahimitabar, Mohammad Reza (Supervisor) ; Nasiriavanaki, Mohammad Reza (Supervisor)
Abstract
Light propagation through turbid media, such as biological tissues, experience scattering due to inhomogeneous distribution of refractive indices of their microscopic structure. Scattering and diffusion of light not only limit many optical imaging techniques, but are also big challenges for telecommunication, spectroscopy, and other optical techniques. Recently many new imaging methods are developed that work in strongly scattering media such as optical coherence tomography, diffusion tomography, and laser speckle velocimetry. In this thesis, we have studied how wavefront shaping techniques can tackle the problem of scattering. By spatially shaping the wavefront of the incident beam, using...
Numerical analysis of heat conduction treated with highly conductive copper oxide nanoparticles In porous media
, Article Special Topics and Reviews in Porous Media ; Volume 7, Issue 2 , 2016 , Pages 149-160 ; 21514798 (ISSN) ; Rabbani, A ; Ayatollahi, S ; Taghikhani, V ; Sharif University of Technology
Begell House Inc
2016
Abstract
In this paper, the effect of highly conductive copper oxide nanoparticles on the effective thermal conductivity (ETC) of rock samples was mathematically investigated. To solve the governing conservation equations for the ETC a commercial finite element package (COMSOL Multiphysics) was used. It should be stressed that the single-phase approach was employed to mathematically model the effect of nanofluid on the heat transfer improvement. The computational geometry of the rock samples was obtained by analyzing the microscopic images of the limestone rock samples. The results obtained from the mathematical modeling of the rock samples showed that the conductive heat transfer through porous...
Investigations on the Effect of Biosurfactants on the Wettability of Carbonate Rocks via Interactions with Rock and Fluid in Ex-Sito MEOR
, Ph.D. Dissertation Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Kamali, Mohammad Reza (Supervisor) ; Shadizadeh, Reza (Co-Advisor)
Abstract
The effects of biosurfactants on sandstone rocks are known in the literature. However, there are few examples of experimental works in Iranian carbonate reservoirs dealing with the effects of biosurfactants under reservoir conditions. This thesis discusses the effect of an efficient biosurfactant produced from Pseudomonas aeroginosa MRo1, a bacterial strain isolated from oil excavation areas in southern Iran, on the recovery of residual oil trapped within carbonate rocks was investigated. The MRO1 could reduce surface tension from 72 to 28 dyne/cm. It also could reduce Interfacial tension from 18.382 to 5.646 dyne/cm.
In a core holder set-up bearing four limestone- and...
In a core holder set-up bearing four limestone- and...
Photocatalytic mechanism of action of apatite-coated Ag=AgBr=TiO 2 on phenol and Escherichia coli and Bacillus subtilis bacteria under various conditions
, Article Progress in Reaction Kinetics and Mechanism ; Vol. 1 , Vol.36 (1) , 2011 , pp.38-52 ; Gholami, M. R. (Mohammad Reza) ; Rahimnejad, S. (Sara) ; Pourbaba, R. (Reza) ; Haghighi, S.(Saeed) ; Sharif University Of Technology
2011
Abstract
Multi-component photocatalysts based on apatite-coated Ag/AgBr/TiO(2) were prepared by the deposition method. The effects of various kinds of apatites, with hydroxyl and fluoro substituents, on photocatalytic activity were investigated. The antibacterial processes in the dark, and under visible light, on two types of bacteria indicate that the multi-composites can inhibit the growth of bacteria by two different mechanisms. TEM images and optical microscopic data demonstrate that by attaching the nanosize catalyst to the outer membrane of the cell, the bacteria could not derive nourishment from surrounding media, i.e. this component acts as bacteria-static. The mechanism for deactivation of...
Solvatochromic Probes Absorbance Behavior in Mixtures of 2-Hydroxy ethyl ammonium formate with methanol, ethylene glycol and glycerol
, Article Journal of Solution Chemistry ; Volume 39, Issue 10 , October , 2010 , pp 1509-1519 ; Harifi-Mood, A. R. (Ali Reza) ; Elahifard, M. R. (Mohammad Reza) ; Gholami, M. R. (Mohammad Reza) ; Sharif University of Technology
2010
Abstract
Solute-solvent and solvent-solvent interactions were investigated for binary mixtures of an ionic liquid (IL) 2-hydroxy ethylammonium formate as with methanol, ethylene glycol and glycerol. The physicochemical properties of the solvent mixtures at 25 °C, over the whole range of mole fractions, were determined using solvatochromic probes. High normal polarity ( ENT ) in the alcohol-rich region confirms solute-solvent interactions in this medium. Dipolarity/polarizability (π ∗) show a different trend to ENT with a positive deviation from ideal behavior in IL-glycerol mixtures. However, these deviations for other solvent mixtures are insignificant. Contrary to what is observed for ENT and π ∗,...