Sharif Digital Repository

This research presents an efficient thermal-aware routing protocol based on Shortest Hop Routing algorithms for implanted bi medical sensor network. Implanted biomedical sensor networks provide a major application of sensor networks in the near future. These in-vivo sensor nodes collect desired biometric data and communicate the data wirelessly to a base-station through a multi-hop network. They generate the heat due to wireless communication and circuitry power consumption. A high temperature of the in-vivo nodes for a prolonged period might damage the surrounding tissues. Most of the protocols for sensor networks don’t take the thermal issue as a factor in the routing of packets. In its... 

Impedance spectroscopy is known as one of the important integrated sensing methods in micro scale Biosensors and electrochemical sensors. Recently, Impedance spectroscopy has been noticed in many micro scale applications. These micro scale applications is being developed specially in bioelectronics and biomedical. The goal of this thesis is designing an on-chip impedance spectroscope system for high frequency with 10 bits resolution via fully electrical blood glucose concentration measurement. This system extracts the blood glucose concentration data through measuring the permittivity coefficient of blood at 1 GHz frequency. In this thesis, new method for high frequency high resolution... 

In this project, quantification of sulfide ion was investigated based on peroxidase enzyme inhibition. The fungal peroxidase was obtained from the liquid culture of Coprinus cinereus NBRC 30628. Two different biosensing systems were suggested for sulfide ion measurement: (1) An amperometric inhibition peroxidase biosensor proposed for determination of sulfide by in situ bulk polymerization of acrylamide for immobilization of peroxidase on screen-printed carbon electrode. Measurements were performed using phosphate buffer solution as a supporting electrolyte in the presence of hydroquinone as a mediator. Identification method was based on reduction of generation current, caused by hydrolysis... 

Diabetes is one of the most catastrophic diseases in the world. There will be an end to patients’ problems if scientists can invent the artificial pancreas, but there is no implantable sensing core available. All of the sensors used for measuring blood glucose level were based on an electrochemical sensor which will generate a glucose level dependent electric current. The chemical structure of that sensor will deny its usability for in vivo applications. By exploring several biological and electrical literatures we introduced a fully electrical method for sensing the blood glucose level by impedance measurement. In this thesis we introduced a new method for sensing the blood glucose... 

The main goal of this project is analysis, design and implementation of scanning near field optical system for detection of biological species. The activities fall in two main category. Theoretical and experimental. In theoretical part, after studying different models describing near field interaction, we have developed software for computationally analysis of nonlinear interaction of light with nanostructures, considering third order nonlinear susceptibility and dispersion behavior of permittivity for metallic nanostructures. The software implements three dimensional finite difference time domain (FDTD) method for analysis of interaction of electromagnetic wave with matter. In developed... 

In this project an enzymatic peroxidase biosensor based on two naono structured material 1-Polyaniline nananofibers 2-Polyethylene glycol-Poly citric acid copolymer was studied for phenol detection.Polyaniline nanofibers and poly ethylene glycol-poly citric acid copolymer were synthetized by chemical polymerization.Electrochemical behaviors of these biosensors were studied using cyclic voltammetry (CV) and amperometric techniques. The amperometric response of the coated electrodes is highly stable with 92% of the initial activity remaining after 5 determinations. These phenol biosensors show an oxidation peak at 0.55V. The amperometric response for phenol biosensor based on Polyaniline... 

Fully integrated frequency synthesizer is one of the important system-on-chips building blocks. Among this circuit’s remarkable applications, Bioelectronics field is very common. In these applications, advanced microelectronic circuits are used in order to diagnosis and treatment of diseases. The aim of this thesis is design and implementation a wideband frequency synthesizer in a 0.18um CMOS technology. The main usage of this circuit is in a fully integrated implantable biomedical sensory chip. This circuit generates two necessary local signals in sensory chip to stimulus cells and process the information.
Frequency synthesizer is based on phased locked loop (PLL) circuit and produce... 

The present work describes sensing application of modified Pt nanostructures towards detection of glucose. Modified Pt nanostructured electrodes were prepared using electrodeposition method such as chronoampermetry and potentiostatic pulse methods. The electrodeposition of Pt nanostructures was first studied by voltammetry and amperommetry techniques and then potentiostatic pulse method has been used to produce the alloyed structures of platinum. In this regard, the first electrode was proposed based on alloyed Pt/Ni nanowire arrays (NWAs) for the non-enzymatic detection of glucose. The Pt/Ni NWAs were prepared by pulse electrodeposition of Pt and Ni within a nano-pore polycarbonate (PC)... 

In this project during 2 sections the synthesis and application of nanomaterials based on carbon nanotubes and graphene oxide for immobilization of Glucose oxide and Horseradish peroxidase enzymes for construction of effective biosensors were investigated. In section 1, we introduce a new nanomaterial in which TiO2-coated carbon nanotubes were functionalized with amine groups (NH2-TiO2-CNTs). This nanomaterial was fixed on a glassy carbon electrode surface and then its ability for direct electron transfer of Glucose oxidase (GOx) and Horseradish peroxidase (HRP) on the modified electrode was investigated. The direct electrochemistry of immobilized GOx and its electron transfer parameters at... 

In the present work, edge-functionalaized graphene was used as electrode material and its electrochemical properties were systematically characterized by electrochemical impedance spectroscopy and cyclic voltammetry. Two methods were applied to modify the gold electrode surface. First method was the self assembling process of graphene nanosheets on gold electrode surface. Second method was simply casting specific amounts of graphene suspension on the gold electrode surface. Nyquist plots show decrease of the charge transfer resistance on the electrode surface at both cases. In cyclic voltammetry studies, first electrode shows no significant changes while the second electrode exhibits... 

In this thesis periodic plasmonic nanostructures are studied for detection of biological specious. The behavior of metal nanostructure arrays under normal incidence has been widely reported. However, simulation of periodic dispersive structures under oblique incidence requires newer formulations. Formulations, and algorithms based on modified split-field finite-difference time-domain (SF-FDTD) method are introduced, permitting analysis of metallic nanostructures arrays under oblique incidence. These novel algorithms are practically implemented on a parallel processing system based on graphics processing unit (GPU). Test and verification of these formulations are done by analyzing referenced... 

Graphene, the newborn nanomaterial whose unique properties has extensively drawnattention of many scientists in the recent years, is an ideal candidate for sensing applications due to its exclusive properties like extremely high specific surface area, excellent electrical conductivity, andcapability of being easily functionalized. In this research, after reviewing the attempts already performed on detection of DNA, in order to detect the four bases of DNA (G, A, T, and C) via electrochemical approach, graphene nanosheets were synthesized by the popular Hummer’s method and were deposited on a graphite rod by electrophoretic deposition with a vertical preferred orientation. To eliminate the... 

This thesis is divided into three parts: In part I, conformational properties of neutral, anionic, cationic and zwitterionic forms of glutathione tripeptide were investigated by means of DFT-B3LYP method with 6-31+G (d,p) basis set. Results show that glutathione is a very flexible molecule and its conformational energy landscape is strongly influenced by forming intramolecular hydrogen bond and its charge. Conformation of each amino acid in glutathione tripeptide depends on its orientation within the peptide sequence in addition to the conformation of other amino acids within the chain. Investigation of intramolecular hydrogen bonds in these conformers by means of AIM analysis demonstrates... 

In this project, the behavior of single strand DNA (ssDNA) that is end-tethred to surface (surface is covered with an layer of mercaptohexane ) through the Monte Carlo simulation has been investigated. To understand this behavior, a ssDNA has been studied through freely jointed chain (FJC) model with electrostatic, base paring, and base-pair stacking interaction. The concentration of sodium chloride salt in solution is 1 mM and the applied surface potential is -1.5 - +0.25 V. Computational results show that the ssDNA chain can have the following configurations: 1) “very closed”, 2) “closed”, and 3) “open” that considering the amount of energy, the mentioned configurations can be compared... 

For application as a biosensor electrode, Aligned Carbon Nanotubes (CNTs) were produced on Stainless Steel 304 substrate by optimizing the CVD method. For better performance, Zinc Oxide (ZnO) nanoparticles were coated on CNTs, using electrochemical deposition method at room temperature and process variables were optimized to acquire discrete particles with a narrow distribution. After that, Polyaniline, which is a conductive polymer, was electropolymerized on CNT/ZnO composite to further improve the electrocatalytic abilities of the electrode. The produced electrode was then used to detect glucose by Cyclic Voltammetry and Amperometry methods. This electrode shows a linear relationship... 

In this research, an amperometric inhibition biosensor for the detection of cyanide has been fabricated by immobilization of the horseradish peroxidase (HRP) on the surface of glassy carbone electrode (GCE). Chitosan/acrylamide was applied for immobilization of HRP on the working electrode. All electrochemical measurement were carried out with three electrode: Ag/AgCl electrode as reference electrode, GCE as working electrode and platinium electrode as counter electrode which immersed in phosphate buffer solution. The amperometric measurement was done at an applied potential of -100 mV versus Ag/AgCl with a scan rate of 100 mV in the presence of hydroquinone as electron mediator and... 

An efficient method has been developed to synthesize well-aligned multi-walled carbon nanotubes (MWCNTs) on a conductive Ta substrate by chemical vapor deposition (CVD). By varying CVD parameters, aligned CNTs (A-MWCNTs) with diameter between 20-100 nm and length of some micrometers with bamboo-like shapes were produced. Free-standing MWCNTs arrays were functionalized and purified through electrochemical oxidation. Then in order to improve the performance, facile template-free electrochemical routes were developed for the shape-selective synthesis of less-common Au nanostructures, including flower, sphere, dendrite, sheet and rod on A-CNTs at room temperature. The results showed that the... 

Design, simulation, optimization and manufacturing of an enzyme-based microfluidic biosensor has been carried out during this research to measure histamine concentration in a sample. The designed biosensor is composed of a micro-bioreactor to degrade histamine and yield hydrogen peroxide, and a chemical reactor to measure the amount of hydrogen peroxide, using chemiluminescence methods. The geometry of the bioreactor and chemical reactor is developed through comparison and optimization of several different geometries. In theory, the designed sensor can determine histamine concentration in a range of 10M to 1mM under one minute, with a maximum error of 5%. Designed biosensor is built using... 

The most important factor in protein's function and its 3D structure is folding. Misfolding or native folding perturbation in some peptides and proteins force them get into amyloid fibril aggregation. It has been observed that these aggregations are the cause of some debilitative diseases especially ones that are related to aging like Alzheimer disease, Hantington's disease, typeII diabets etc. Recently it has been shown that specific regions in human catalase protein interact with some amyloid fibrils, such as Aß, IAPP, PrP etc. Inteins are self-splicing protein enzymes, which excise themselves from the mature protein and join the two flanking adjacent sequences by a new peptide bond in a... 

In this research, fabrication of a mediator free nano-biosensor for the detection of cyanide has been studied. This biosensor utilizes 75 nm diameter (nano) ZnO particles synthesized with sol- gel method for modification and immobilization of Horseradish peroxidase (HRP). Cyanide plays inhibitory role in decomposition of H2O2 while HRP plays catalyst role in this reaction. Cyanide inhibits enzyme activity thorough binding with enzyme active sites and result in decreasing electrical current in electrochemical decomposition of H2O2. Amperometery response of biosensor shows linear relation between inhibition percent and cyanide concentration in range of 3µM - 24µM with detection limit of 0.62µM...