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Synthesis and Characterization of Zeolitic Imidazolate Framework-8 as a Carrier for Gene Therapy Applications
, M.Sc. Thesis Sharif University of Technology ; Simchi, Abdolreza (Supervisor) ; Behmanesh, Mehrdad (Co-Supervisor)
Abstract
Advances in genetics and biology have led to the ability to produce therapeutic genes and modify defective genes in patients' genomes, resulting in a solution to many genetic diseases. Many genetic diseases can be overcome through gene therapy by transferring therapeutic genes into the cell nucleus. However, researchers have not yet been able to develop a safe, high-efficiency carrier for the transfer of genes to the target cell, and this has become a bottleneck for the clinical applications of gene therapy. In this study, the ZIF-8 organic-metallic framework was used as a new vector for the transfer of therapeutic genes to target cells. For this purpose, ZIF-8 was synthesized by a...
Evaluation of Plasmid Transmission in Bacillus Subtilis by Cell Wall Modification
, M.Sc. Thesis Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Aghamollaei, Hossein (Supervisor)
Abstract
Today, almost all the country's needs in the field of industrial enzymes are met through imports. In this regard, much attention has been paid to the industrial production of various enzymes in the country. The production of recombinant proteins through strain engineering is one of the most important ways to meet this demand due to its many benefits. One of the most important microorganisms that is widely used in the enzyme industry is Bacillus subtilis. Due to its thick peptidoglycan wall, this gram-positive bacterium hardly accepts engineered carriers for genetic modification as hosts. The aim of this study was to evaluate the permeability of the wall and cell membrane of Bacillus subtilis...
Synthesis and Characterization of Transition Metal Complexes Supported on Carbon-Based Nanocomposites and Their Application as Gene and Drug Delivery Systems
, Ph.D. Dissertation Sharif University of Technology ; Bagherzadeh, Mojtaba (Supervisor)
Abstract
In this dissertation, an attempt has been made to optimize some smart nanocarriers and the controllable release of drug (doxorubicin) and genetic material (plasmid CRISPR). Regarding, some metal nanoparticles, metal-organic frameworks, transition metal complexes, graphene oxide, and functionalized carbon nanotubes have been synthesized and also characterized and their hybrids and nanocomposites have been prepared successfully. All the abovementioned nanomaterials were designed and synthesized due to achieve biodegradable, biocompatible, higher positive surface potential, lower cytotoxicity, better responsivity to stimulus, and greener compounds. Doxorubicin and plasmid CRISPR has been...