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Synthesis and Biological Evaluation of Superparamagnetic Iron Oxide Nanoparticles as Contrast Agents for Bioimaging Applications

Ali Abouzar, Mitra | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 41469 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Maddah Hosseini, Hamid; Oghabian, Mohammad Ali
  7. Abstract:
  8. A biocompatible ferrofluid containing ?Fe?_3 O_4 nanoparticles was produced using co precipitation of ?FeCl?_2.?4H?_(2 )O and ?FeCl?_3 ?6H?_2 O under ultrasonic irradiation power with NaOH being the alkaline. Two variables studied in this research were concentration and molecular weight of PEG, as the coating agent. The impact of these variables were carefully monitored on shape, size distribution and magnetic behavior of nanoparticles through SEM, DLS, XRD and VSM characterization techniques. Moreover, MRI relaxation times,T_1and T_2, were calculated using synthesized nanoparticles as contrast agents. Accordingly these nanoparticles were biologically evaluated by MTT assay. Results indicated that nanoparticles produced under ultrasonic irradiation via coprecipitation had a narrow size distribution and proper saturation magnetization for bioimaging applications. It was also clear from SEM and DLS images that the more the PEG concentration, the higher the hydrodynamic diameter. Using these nanoparticles as contrast agents for MRI images helped reduce the relaxation times compared to water, causing optimal contrast for detection and diagnosis. MTT assays revealed satisfactory cell viability (%) for tested samples in 3-h and 24-h of incubation. However, viability decreased as incubation time and Fe concentration increased
  9. Keywords:
  10. Ferrofluid ; Co-Precipitation ; Relaxation Time ; MTT Assay ; Viability

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