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Experimental Removal of Diesel in Soil Using Indigenous Microorganisms Isolated Iran

Rezaei, Nader | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42401 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Kariminia, Hamidreza; Yaghmaei, Soheila
  7. Abstract:
  8. Crude oil and petroleum products at the time of extraction, production and transportation cause serious contaminations in soil, water and environment. Biological methods have shown a promising effect for the cleanup of these hydrocarbon pollutants. Bioremediation employs the metabolic activity of microorganisms capable of using oil as their energy and carbon source. Biological methods are also less expensive than other cleanup technologies such as incineration and landfill. Large number of microbial stains have been discovered in nature, capable to degrade oil and petroleum products. Biological cleaning of oil contamination by microorganisms occurs naturally in the environment. It has been estimated that petroleum hydrocarbon degrading bacteria existing in soil and water, include about one percent of the total microbial population in the world. The population of these bacteria increases in the presence of hydrocarbons and reaches to about ten percent of the total microbial population in the contaminated area. The objective of the present research is to investigate the removal of diesel as a contaminant, from soil using indigenous soil bacteria previously isolated from polluted sites in Iran. It was observed that, the four bacterial strains selected among the isolates, were all capable of using diesel as the sole carbon source in the soil phase. Over 70% of diesel removal was observed in 30 days for these strains, when the initial concentration of diesel was 15,000 mg diesel/kg soil. Strain Sh-3, exhibited the highest diesel removal efficiency of 80%, that is comparable with other researchers findings. A mixed culture of the 4 selected strains also resulted in nearly 80% contamination removal. However, the removal rate was increased compared for the mixed culture. The effect of different type of surfactants (anionic, cationic and non-ionic) was investigated and no major improvement in the diesel removal efficiency was observed. Microbial growth kinetic parameters were also determined using Michaelis-Menten equation
  9. Keywords:
  10. Diesel Fuel ; Biological Treatment ; Soil ; Biological Degradation ; Bioremediation ; Baeterial Suspension

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