Loading...

Characterization of Essential Oil Components of Green and Black Cardamoms Using GC-MS Combined with Chemometric Resolution Techniques & Quantification of Two Types of Taxus Using Fast Liquid Chromatography Combined with MCR

karimi, Mehdi | 2011

739 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42783 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Jalali Heravi, Mehdi
  7. Abstract:
  8. Progress in analysis of multicomponent mixtures and processes focused on the interpretation of the multivariate responses, rely on the combination of sophisticated instrumental techniques and suitable chemometric methods. Among the present chemometric method, curve resolution techniques have a wide application in the analysis of multicomponent mixtures and processes. Chemometric resolution techniques are used in the present work. At the first, Essential oil of green and black cardamoms was extracted by hydrodistillation and was analyzed by gas chromatography–mass spectrometry (GC-MS). Due to the fact that the essential oils are complex mixtures, strong overlap is observed between the peaks. Multivariate curve resolution (MCR) techniques were applied for resolving coeluted GC-MS peak clusters. Various MCR methods such as morphological scores (MS) and fixed size moving window evolving factor analysis (FSMWEFA) were used to determine the number of components, pure variables, and selective regions. The peak clusters were resolved into pure chromatograms and pure mass spectra by using multivariate curve resolution-alternating least square (MCR-ALS) technique. The results showed that MCR-ALS is a good method for resolving peak overlap and determining embeded peaks. Also the use of MCR techniques extends the number of essential oil components and combining these methods with hyphenated chromatographic techniques produce a more accurate and reliable results. In the second part of the contribution, the fast liquid chromatographic method was used for quantification of 10DABΙΙΙ and Taxol in the methanol extract of yew plant in less than 5 min using mobile phase of Water-Acetonitrile (80-20) with flow-rate of 1 mL min−1. Corresponding standards were also analyzed in the same conditions. Appropriate baseline correction through Kvalheim and Liang method was applied to reduce the background noise. Then, second-order calibration based on MCR-ALS technique was implemented on the data obtained from the liquid chromatography with diode array detection (LC-DAD) for resolving co-eluted peaks. Statistical parameters such as coefficient of determination, calibration error curve and relative Standard deviation were calculated. To ensure that the results are accurate, measurements were repeated with ultra performance liquid chromatography (UPLC). As a result of using fast liquid chromatography combined with curve resolution techniques the time of analysis and also solvent consumption were reduced, compared to the analysis in the optimum conditions.
  9. Keywords:
  10. Gas Chromatography/Mass Spectrometry (GC/MS) ; High Performance Liquid Chromatography ; Diode Detector ; Chemometrics Method ; Curve Resolution ; Ultra Performance Liquid Chromatography ; Taxol ; Cumin ; Black Cardomom

 Digital Object List

 Bookmark

No TOC