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Investigating the Optimum Conditions for Cell Growth and Behavior on Hydrogel Surfaces

Hajiabbas, Maryam | 2012

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42887 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohre; Maghsudi, Vida
  7. Abstract:
  8. Generally, the concept of producing ‘spare parts’ of the body for replacement of damaged or lost organs lies at the core of the varied biotechnological practices referred as tissue engineering. Tissue engineering is an interdisciplinary field that incorporates principles of engineering with the life sciences. Tissue engineering is based on three principle; cells, scaffolds for cells expansion, attachment as an environment like ECM and growth factors. These things together can help tissue engineers to provide microenvironments which are suitable for special cells. The most important thing in this kind of works is the ability to simulate environment for cells the same as body. According to the advantages of using hydrogel and combined scaffolds especially combination of poly saccharide and protein polymers in tissue engineering, we tried to make 2D hydrogel sheets scaffold from chitosan and gelatin. The project has an initial goal of evaluating the following set of polymers, to see if they could form suitable thin sheets which would then be used for cell seeding which have good handling for surgery. After preparing hydrogel sheets we had a goal of characterization of Smooth Muscle Cells and Muscle-Derived Cell (MDCs) behaviors on biocompatible polymeric hydrogels. (Investigating cells attachment, expansion, morphology, viability and proliferation) The results show that hydrogel sheets which contained chitosan2% and gelatin 6 %( w/w) has the good handling and can provide the best environment for cells attachment, growth and proliferation.

  9. Keywords:
  10. Tissue Engineering ; Scaffold ; Hydrogen ; Chitosan ; Gelatin

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