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Producing Biodiesel from Waste Frying Oil Using Whole Cell Biocatalysts

Ghaderinezhad, Fariba | 2011

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 43048 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Yaghmaei, Soheyla; Kariminia, Hamid Reza
  7. Abstract:
  8. Nowadays, due to depletion of fossil fuels and the emission of green house gases, biodiesel has attracted lots of attention as a renewable, non-toxic, and biodegradable fuel. Biodiesel can be produced from different resources such as vegetable oil, animal fat and algae oil. In this study, waste frying oil was used as a raw material that can reduce the probable hazardous effects of its disposal and produce a clean fuel at the same. Biofuel was produced through an enzymatic method using the whole cell of Rizopu oryzae (a filamentous fungus) in both immobilized and free forms. In order to find the optimum conditions for biodiesel production, four variables including temperature, methanol to oil molar ratio, the amount of biomass, and rotational speed of shaker were considered as effective parameters. Experimental design using response surface methodology coupled with central composite design was employed for the optimization. Each variable was investigated in three surfaces and the purity of the biodiesel was considered as the response. The optimal value of the parameters in case of using immobilized or free microorganisms were obtained. The experimental data was well-fitted with a quadratic equation used for the statistical optimization. The optimum condition in immobilized and free cell cases was: 1 g microorganism in both cases, temperature 44 and 45○C, 5:1 and 4٫7:1 molar ratio of methanol to oil, and 150 and 160 rpm rotational speed of shaker, respectively
  9. Keywords:
  10. Biodiesel ; Used Frying Oil ; Experiments Design ; Optimization ; Response Surface Method ; Whole Cell

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