Experimental Study and Optimization of Bioremediation of PAHs by Newly Isolated Thermophilic Filamentous Fungi in Slurry Bioreactor

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Sarayloo, Ehsan | 2012

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 43157 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Vossoughi, Manouchehr; Alemzadeh, Iran
Abstract:
Sustainable development requires the development and promotion of environmental management and a constant search for green technologies to treat a wide range of aquatic and terrestrial habitats contaminated by increasing anthropogenic activities. In this work we investigated the ability of four previously isolated fungi, BBRC 9057, BBRC 20020, BBRC 9058 and BBRC 9002, in degradation of polycyclic (PAHs) and the effect of UV irritation on fungi cell growth and degradation of PAHs. Also we studied the degradation of anthracene by thermophilic filamentous fungi in liquid and soil slurry bioreactor. Cell growth and anthracene degradation ability of BBRC 9057 increased 6.51 and 1.82 times rather than parental strain when exposed to UV for 10 minutes.
The effect of temperature and rotation speed on the degradation process was studied in the shaking flasks. The maximum degradation of anthracene (94.7) had done by strain BBRC 9057 at 150 rpm and 50 ◦C.
Finally we investigated the effect of pH and temperature on degradation of anthracene by BBRC 9057 in soil slurry bioreactor. At 45 ◦C and pH=5 anthracene was completely degraded by BBRC 9057
Keywords:
Bioremediation ; Polynuclear Aromatic Hydrocarbons (PAHs) ; Thermophilic Bacteria ; Slurry Bioreactor