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Mechanochemical Synthesis of Copper Doped Nanostructured Fluorapatite
Nikonam Mofrad, Raheleh | 2012
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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 43967 (07)
- University: Sharif University of Technology
- Department: Materials Science and Engineering
- Advisor(s): Sadrnezhad, Khatiboleslam; Vahdati Khaki, Jalil
- Abstract:
- Fluorapatite (FA) has been widely used on orthopedic and dentistry prosthesis due to its excellent bioactivity properties. Therefore, the aim of this work is to prepare and characterize copper doped nanostructured fluorapatite powder via mechanical alloying (MA) method using a high energy planetary ball mill.FA powder samples with the general chemical formula Cux.Ca(10-x).(PO4)6.F2 (where x is the ratio of substitution of Cu-2 by F−1) are successfully synthesized using the starting materials of calcium oxide (CaO), phosphorous pentoxide (P2O5), calcium fluoride (CaF2), and copper (II) oxide (CuO) powders under various milling times. In order to evaluate the antibacterial effect of copper, the x values are set to 0 – 0.4 – 0.9.The various experimental techniques like x-ray diffraction (XRD), Fourier transform infrared (FT - IR), atomic adsorption spectrophotometer (AAS), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FE-SEM) are utilized in order to characterize the synthesized FA powder samples .Moreover, in vitro tests of copper-doped nanostructured fluorapatite powder are performed by immersing the prepared FA in the simulated body fluid (SBF) and the antibacterial effect of the samples against Staphylococcus aureus was observed by the disc diffusion test method.
The XRD results indicates that the nanostructured fluorapatite could be synthesized with the high purity after 48 hours milling for the substitution ratios of 0 and 0.4, with the respective crystallite size of 22nm and 17nm. However, the synthesis time mounts to 72 hours in case of nanostructured fluorapatite with substitution ratio of 0.9 and crystallite size of 14nm.
Additionally, both FA and CuFA samples have bioactivity properties and CuFA has comparatively less solubility than FA in SBF solution. While high antibacterial properties are discerned for all the three samples containing different amount of copper, when exposed to Staphylococcus aureus bacteria, a positive correlation between copper content and the anti-bacterial properties is identified - Keywords:
- Nanostructure ; Antibacterial Properties ; Mechanochemical Alloying Process ; Copper Doped Nanostructured Floorapatite
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