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Dehghan, Mohammad Reza | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 44325 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Aalemzadeh, Iran; Maghsoudi, Vida
  7. Abstract:
  8. Xanthan gum is a water-soluble exo-polysaccharide. It is produced industrially from carbon sources by fermentation using the gram-negative bacterium Xanthomonas campestris. There have been various attempts to produce xanthan gum by fermentation method using bacteria and yeast by using various cheap raw materials. In this study the cultural conditions for xanthan gum production by Xanthomonas campestris were investigated and optimized by response surface methodology, to maximize cell and xanthan production in batch experiments using YM broth without pH control. The individual and interactive effects of three independent variables (carbon source concentration (40-80 g/l), nitrogen source concentration (1-5 g/l), temperature (25–35 °C)) on xanthan gum and biomass production were studied, using a face-centered composite design of experiments. Glucose was used as carbon source and di ammonium sulfate as nitrogen sorce. A second ordered polynomial model was fitted and optimum conditions were estimated and optimum conditions were estimated. Overall optimization allowed us to point out an optimal range of the three independent variables. Optimal xanthan gum production was found at 80 g/l glucose concentration, 3 g/l di ammonium sulfate and 30°C.
    Keywords
  9. Keywords:
  10. Carbon Source ; Themperature ; Response Surface Methodology ; Xanthan Gum ; Xanthomonas Campestris Bacteria ; Nitrogen Source

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