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Experimental Investigation on Growth and Control of Animal Cells Attached on Scaffold

Solmaz, Naseri | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 44668 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vossoughi, Manoochehr; Alamzadeh, Iran
  7. Abstract:
  8. Major bone defects resulted from diseases or hurts that can not heal naturally, requaire bone transplantation. golden standard for transplantation is autologus one but it has some disad-vantages such as economic burden, the lack of graft source, surgery on patient, and so on. so exploring altrnative ways led to exploit stem cells.traditionally bone marrow mesenchymal stem cells (MSCs) used for stem cell-bsaed bone regeneration. bone marrow aspiration to aquaire stem cells is painfull and give a population of cells such as hematopoetic cells that requaire to seperate and can not be handled in first passages. MSCs can be substituted by multipotent adipose tissue-derived stem cells (ADSCs) in bone repair because of ablility of differentiate to osteoblasts, easily accesable, not need to surgery. in vitro, ADSCs requaires osteogenic medium and a substrate like ECM 1 to adhere, proliferate and differentiate. in this study ADSCs were cultured on 4 types of electrospun scaffolds to examine effect of scaffold on differentiation and mineralization. first biocampatibility were tested using MTT assay then differentiation and mineralization were examined by ALP assay on day 0, 4, and 10 and on day 10 by Alizarin Red staining, respectively. 3-D scaffolds mimic ECM well and their porosity promotes cell proliferation, adhesion and migration. effect of these type of 3 layer electrospun scaffolds on the osteoblastic differentiation was studied and showed ele- vated level of the expression of the genes specific to the osteoblasts like RUNX2, Collagen type I, and Osteopontin using Real Time-PCR
  9. Keywords:
  10. Stem Cell ; Biocompatibility ; Differentiation ; Mineralization ; Alkaline Phosphatas Assay ; In-Vitro ; MTT Assay

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