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Experimental Studies on the Application of Biosensor for Detection of Toxic Compound

Ghanavati, Mehdi | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45086 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Roosta Azad, Reza; Musavi, Abbas
  7. Abstract:
  8. In this research, an amperometric inhibition biosensor for the detection of cyanide has been fabricated by immobilization of the horseradish peroxidase (HRP) on the surface of glassy carbone electrode (GCE). Chitosan/acrylamide was applied for immobilization of HRP on the working electrode. All electrochemical measurement were carried out with three electrode: Ag/AgCl electrode as reference electrode, GCE as working electrode and platinium electrode as counter electrode which immersed in phosphate buffer solution. The amperometric measurement was done at an applied potential of -100 mV versus Ag/AgCl with a scan rate of 100 mV in the presence of hydroquinone as electron mediator and 0.1 M phosphate buffer solution of pH 7.5. The parameters which can affect the performance of biosensor including the amount of substrate (H_2 O_2), hydroquinone concentration as mediator and PH electrolyte were optimized. The determination of cyanide can be achieved in a linear range of 1.64–13.13 μM with a detection limit of 0.43 μM. Common anions and cations in environmental water did not interfere with cyanide detection by the developed biosensor. Sulfide interference on the enzyme inhibition caused 51.96% error in the calibration assay. Because of high sensitivity and the low-cost of this biosensor, this inhibition biosensor can be successfully used for analysis of environmental water samples
  9. Keywords:
  10. Inhibition ; Cyanide Determination ; Amperometric Method ; Enzymatic Inhibition ; Biosensor

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