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Experimental Study and Prediction of Vancomycin Partitioningin Surfactant-Salt Aqueous Two-Phase Systems

Shirvani, Sara | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45921 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosughi, Manouchehr; Rashtchian, Davood; Pazuki, Gholamreza
  7. Abstract:
  8. In the past years, upstream processes have been receiving a considerable R&D investment and commercial attention. The downstream processes have, however, been overlooked causing a production bottleneck that is shifting the costs of production from up to downstream. The need to develop more efficient and cost-effective separation and purification processes is, thus, crucial in order to improve process efficiency and economics keeping the required high standards of quality for market approval. In this research, promising aqueous two-phase systems (ATPS) containing Tween 20 as a quite biocompatible non-ionic surfactant and different salts (K3Citrate and NaH2PO4) were prepared and their extractive potential were evaluated. These ATPS possess top and bottom phases well-stocked in surfactant and salt which can offer appealing characteristics such as low interfacial tension, low volatility and ease of scaling-up. Ternary phase diagrams of ATPS consisting of Tween 20 and two different salts were obtained and the results were successfully fitted to Merchuck equation. The salting-out ability of salts were assessed through the effective excluded volume (EEV) parameter. Also, the tie-line data were correlated with the Othmer-Tobias and Hand equations. Afterwards, central composite design (CCD) combined with response surface methodology (RSM) have been applied to correlate and optimize the partition coefficient of Vancomycin. The effect of four process variables, including temperature, Tween20 wt%, salt wt% and Vancomycin wt% as well as their binary interactions, were modeled. The influences of K3Citrate and NaH2PO4 on partition coefficients were justified by Hofmeister series. The experimental data ofVancomycin partition coefficients indicated the proper potential of both systems, especially Tween 20-NaH2PO4-water biphasic system at high weight percent of Tween and salt, for bio separation process
  9. Keywords:
  10. Salts ; Optimization ; Surfactants ; Aqueous Two-Phase System ; Response Surface Method ; Vancomycin Antibiotic

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