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Preparation of Gelatin-Alginate Hydrogel for Using as Cell Culture Scaffold

Fadaodini, Samira | 2013

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45166 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Mashayekhan, Shohreh; Maghsoudi, Vida
  7. Abstract:
  8. Hydrogels are polymeric three-dimensional networks able to swell in the presence of an aqueous medium. Hydrogels from natural proteins and polysaccharides are ideal scaffolds for tissue engineering since they resemble the extracellular matrices of tissue comprised of various amino acids and sugar-based macromolecules.The biocompatible and biodegradable hydrogel scaffolds are promising materials for tissue engineering. Here, we report a new class of hydrogels derived from oxidized alginate (OA) and gelatin. The prepared oxidized alginate was shown to be efficient in crosslinking gelatin, leading to hydrogel formation. The effect of degree of oxidation and concentration of OA on the mechanical properties of the prepared hydrogels was examined. The hydrogel young mudulus (E) increased with increase in concentration of OA, and increase in the degree of alginate oxidation. To demonstrate the cell behavior of cultured mesenchymal stem cells (MSCs) on the gel matrixes, the colorimetric MTT metabolic activity assay was performed. In general, density of cells was found to increase with decrease in OA concentration. It was found that the degradation behavior of OA-gelatine hydrogels relied heavily on the degree of alginate oxidation. The results obtained in the biological characterization allow to select the OA-gelatin 30:70 hydrogel as a suitable material to prepare 2D scaffolds for tissue engineering
  9. Keywords:
  10. Hydrogel ; Scaffold ; Gelatin ; Cell Culture ; Oxidized Alginate

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