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A rapid and sensitive HPLC-fluorescence method for determination of mirtazapine and its two major metabolites in human plasma

Lavasani, H ; Sharif University of Technology

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  1. Type of Document: Article
  2. Abstract:
  3. A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethyl mirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH = 3, 20:80, v/v) in isocratic mode at a flow rate of 2 mL/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/mL for MRZ and 2 ng/mL for both NDM and 8-OHM. The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer
  4. Keywords:
  5. Mirtazapine ; Metabolites ; HPLC ; Plasma ; Fluorescence
  6. Source: Iranian Journal of Pharmaceutical Research ; Volume 13, Issue 3 , Summer 2014 , Pages 853-862
  7. URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177645