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Modeling and Engineering Design of Fluidic and Optical Parts of a Flow Cytometer Device

Yazdani, Amir | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46938 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saeedi, Mohammad Saeed
  7. Abstract:
  8. Nowadays counting small particles and gathering their morphological information is widely used in many fields as medicine, engineering, etc. One of the best examples of this technology is counting and distinguishing different cells which are considered bioparticles. In this thesis a brief review of different particle counting methods is presented. Among these methods flow cytometry technology is chosen, because of its speed and accuracy. In flow cytometry method cells are conducted to the center of a transparent capillary by sheath flow technique; then laser beam is radiated to the cells. The intensity of the light scattered from the cells is used to determine cell type and count. In following chapters, fluidic system of this device is designed using analytical and numerical solutions of flow field and validated by experimental tests. Light scattering pattern of different blood cells is modeled using DDA numerical method. These light scattering results is used to design a new and optimized optical system which does not need fluorescent dyes to distinguish blood cells. This new design reduces the running cost of a flow cytometer device because the fluorescent dyes are the most expensive materials needed to do a test with a flow cytometer device
  9. Keywords:
  10. Computational Fluid Dynamics (CFD) ; Light Scattering ; Discrete Dipole Approximation ; Sheath Flow ; Flowcytometry

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