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Investigation of biotechnological production of Vitamin C

Alizad Derakhshi Azar, Sepideh | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 46998 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran
  7. Abstract:
  8. Vitamin C is an essential for the survival of human, animals and plants. Human and most animals can not produce vitamin C in their bodies, thus the need to produce vitamin C as a supplement is necessary. Nowadays, for the industrial production of vitamin C, two Chinese and Rychstyn methods are used, the first method is a two-step method which is accomplished biological and the second method is done chemically. In Chinese method, first step, which is the most important step is production of L-sorbose from D-sorbitol by Gluconobacter oxydans bacteria .Thus, in this study it was attempted to optimize the production of L-sorbose to be used in industrial production of L-ascorbic acid. In this regard test design software, Design Expert 7, with response surface methodology was used to optimize concentrations of materials of culture medium and inoculum percent of Gluconobacter oxydans and this bacteria was immobilized in Calcium alginate beads to be used in semi-continuous operations in reactor. For keeping both substrate and oxygen permeation and mechanical strength of beads at high level, optimal concentration of sodium alginate solution during the investigations was obtained 2.5 g per 100 ml of deionized water and bead diameter was 3.1mm. The maximum amount of target compound was produced by free cells under optimal conditions and the amount was 43.4 grams per liter of deionized water. The maximum production efficiency was obtained 28.6%. The maximum amount of L-sorbose produced in reactor using immobilized cells was measured 37.4 grams per liter of deionized water. All culturing operations were accomplished at T=30℃ and initial pH of 5.1
  9. Keywords:
  10. Response Surface Method ; Vitamin C ; D-Sorbitol ; L-Sorbose ; Calcium Alginate Beads ; Gluconobacter Oxydans Bacteria

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