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Conjugated Linoleic Acid Microbial Production

Toori Karami, Hadiseh Sdat | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 47718 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Alemzadeh, Iran
  7. Abstract:
  8. Conjugated linoleic acid (CLA) is a collective term used to describe the mixture of geometric isomers of linoleic acid which has several beneficial biological effects. Considering the use of CLA for medicinal of nutraceutical purposes, an isomer-selective and safe process is required. The introduction of biological reactions to CLA production will solve this problems. Nowadays, CLA is produced through chemical isomerization of linoleic acid which results in by-production of unexpected isomers. From this point of view biotechnology methods and producing microorganisms implementation have gained lots of attention because of not producing unwanted bypass products and also being natural method. Goal of this research is CLA microbial production and investigation of process parameters on CLA production enhancement. Effect of reaction period, oil concentration and washed cells weight ratio on production of CLA by Lactobacillus plantarum PTCC1058 and Lactobacillus plantarum subsp.plantarum PTCC1745 were measured. For this purpose Design-expert software was used. Lactobacillus plantarum PTCC1058 bacteria had more yield than Lactobacillus plantarum subsp.plantarum PTCC1745. The highest yield was achieved for Lactobacillus plantarum PTCC1058 in reaction mixture including 15% (w/v) washed cells, 4.6(mg/ml) castor oil, buffer phosphate (pH=6.5), tween80 and in presence of lipase enzyme and reaction period of 121 hours. This yield was equal to 36.11% of CLA production yield
  9. Keywords:
  10. Gas Chromatography/Mass Spectrometry (GC/MS) ; Lactobacillus Acidophilus Bacteria ; Conjugated Linoleic Acid (CLA) ; Microbial Production

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