Design and Fabrication of Hydrogel Microcarrier for Tissue Engineering

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Radaei, Payam | 2014

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 47942 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Mashayekhan, Shohre; Yaghmaei, Soheila
Abstract:
Microcarriers (MCs) are an attractive technology with various applications in tissue engineering. In this study, chitosan/gelatin MCs were fabricated with blends of different ratio of chitosan/gelatin, by using a setup containing high voltage electrostatic field and syringe pump. Optimization of blend ratio, voltage and syringe pump flow carried out by the “Design expert” software leads to fabrication of MCs with constant diameter while having various elasticity. Mechanical strength and elasticity of MCs were determined. Human umbilical cord mesenchymal stem cells (hUCMSCs) were cultured on MCs dynamically by using mini-rocher in an incubator. Cells adhesion on MCs were successfully shown by MTT assay and cells morphology on the MCs was observed by invert microscopy. Finally the prepared MCs were optimized physically and biologically to achieve the desired cell attachment and proliferation on the beads
Keywords:
Tissue Engineering ; Hydrogel ; Chitosan ; Gelatin ; Microcarrier ; Mesenchymal Stem Cell Diffentiation

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