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Synthesis of Hydroxyapatite Whisker and Nanorods/Polycaprolactone Composites and Scaffolds: In vitro & In vivo studies

Lebasi, Mohammad Ali | 2015

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  1. Type of Document: M.Sc. Thesis
  2. Language: English
  3. Document No: 47923 (57)
  4. University: Sharif University of Technology, International Campus, Kish Island
  5. Department: Science and Engineering
  6. Advisor(s): Mohammadi, Mohammad Reza
  7. Abstract:
  8. This work describes the effect of hydroxyapatite with different morphology including nanorods and whisker on the properties of hydroxyapatite/polycaprolactone (HA/PCL) composite. Biodegradable polymer/Hydroxyapatite (HA) composites with the morphology of nanorods and whisker of (HA) as bone replacement scaffolds are synthesized by sol-gel and hydrothermal methods, respectively with a Ca/P ratio of 1.67 and 20:80 (HA/PCL).The crystallization behavior and porosity of HA/PCL composite are studied by scanning electron microscope and X-Ray diffraction. The response of bone marrow-derived human mesenchymal stem cells (hMSCs) in terms of cell proliferation and differentiation to the osteoblastic phenotype was evaluated using the alkaline phosphatase activity. It was found that the ALP activity increased in a statistically significant manner until day 21 and after that increased slowly toward the day 28. In the present study, we investigated PCL/HA scaffolds in vitro and in vivo performance by means of hydrolytic degradation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay, as well as repair the calvarial defects in clinical white rabbits. The results demonstrated that the degradable scaffold held good biocompatibility. The cell number of chondrocytes increased remarkably in one week, following a relatively slow increase during days 14–28. Qualitative analysis of bone regeneration process was performed by radiological examination. The results indicated that new bone formed originally from the margin of host bone, and then grew toward the center of defects. At 20th week, the defects of treatment were covered with the new bone
  9. Keywords:
  10. Hydroxyapatite ; Nanorod ; Polycaprolactone Composite ; Bone Tissue Engineering ; Whisker

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