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Investigation of optimum fermentation condition for PHA production by four species: Hydrogenophaga pseudoflava DSMZ 1034, azohydromonas lata DSMZ 1123, cupriavidus necator DSMZ 545, Azotobacter beijinckii DSMZ 1041

Karbasi, F ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. DOI: 10.5829/idosi.wasj.2012.20.12.1414
  3. Publisher: 2011
  4. Abstract:
  5. Batch cultures of Azohydromonas lata DSMZ 1123, Hydrogenophaga pseudoflava DSMZ 1034, Cupriavidus necator DSMZ 545 and Azotobacter beijinckii DSMZ 1041 were investigated for producing the intracellular bioplastic poly-•-hydroxybutyrate (PHB). The effects of temperature, seed age, type of carbon sources and the agitation rate on the production rate of PHB were investigated using four mentioned spices. The optimized shaking rate, temperature and seed age were obtained 250 rpm, 30°C and 15 hrs for H. pseudoflava DSMZ 1034 and A. beijerinckii DSMZ 1041 and 18 hrs and 24 hrs for A. lata DSMZ 1123 and C. necator DSM 545, respectively.The effect of carbon sources including glucose, fructose, whey and molasses on biopolymer production rate were studied in this investigation. The maximum productivity of PHB production using fructose as a carbon source was obtained after 72 hrs by C. necator DSMZ 545. In this case, the maximum cell dry weight (CDW) and produced polyhydroxybutyrate were 10.2 g.LG• 1 and 5.8 g.LG• 1, respectively
  6. Keywords:
  7. Batch fermentation ; Biodegradable ; Bioplastic ; Biopolymer ; Poly-•-hydroxybutyrate (PHB) ; Polyhydroxyalkanoate
  8. Source: World Applied Sciences Journal ; Volume 14, Issue SPL ISS 3 , 2011 , Pages 36-47 ; 18184952 (ISSN)
  9. URL: http://www.idosi.org/wasj/wasj20%2812%2912/19.pdf