Optimization of Cell Proliferation on Polymeric Carriers Composed of Accelular Heart Matrix

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Ghanbari Asl, Sasan | 2015

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 47915 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Mashayekhan, Shohreh; Abd Khodaei, Mohammad Jafar
Abstract:
Following heart coronary artery occlusion, heart stroke (HS) happens which leads to the creation of a dead zone on heart tissue named Myocardial Infarction (MI), the presence of which on a patient’s heart will result in succeeding HSs and the death of the patient. In this study, porous microcarriers capable of being utilized in cardiovascular tissue engineering is fabricated using a mixture of myocardium ectracellular matrix (ECM) and Chitosan (Cs). Results of Elasticity tests, SEM images, swelling behavior, biodegradability test, and cell proliferation assay showed that the scaffold consisting of 3.5% (w/w) Chitosan and 0.66% (w/w) ECM has the best potential in providing cardiovascular progenitor cells (CPCs) with a suitable environment in which they can grow and proliferate well. Results, also, proves that the aforementioned microcarrier has high potential in being used in cardiovascular tissue engineering for treating MI
Keywords:
Microcarrier ; Chitosan ; Extracellular Matrix ; Decellularization ; Heart Tissue Engineering

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