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Evaluating the toxic effect of an antimicrobial agent on single bacterial cells with optical tweezers

Samadi, A ; Sharif University of Technology | 2015

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  1. Type of Document: Article
  2. DOI: 10.1364/BOE.6.000112
  3. Publisher: OSA - The Optical Society , 2015
  4. Abstract:
  5. We implement an optical tweezers technique to assess the effects of chemical agents on single bacterial cells. As a proof of principle, the viability of a trapped Escherichia coli bacterium is determined by monitoring its flagellar motility in the presence of varying concentrations of ethyl alcohol. We show that the “killing time” of the bacterium can be effectively identified from the correlation statistics of the positional time series recorded from the trap, while direct quantification from the time series or associated power spectra is intractable. Our results, which minimize the lethal effects of bacterial photodamage, are consistent with previous reports of ethanol toxicity that used conventional culture-based methods. This approach can be adapted to study other pairwise combinations of drugs and motile bacteria, especially to measure the response times of single cells with better precision
  6. Keywords:
  7. (170.3650) Lifetime-based sensing ; (170.4520) Optical confinement and manipulation ; (350.4855) Optical tweezers or optical manipulation ; Antimicrobial agents ; Bacteria ; Ethanol ; Time series ; Toxicity ; Correlation statistics ; Culture-based methods ; Escherichia coli bacteria ; Lifetime-based sensing ; Optical confinement and manipulation ; Optical manipulation ; Pair-wise combinations ; Proof of principles ; Optical tweezers ; Alcohol ; Antiinfective agent ; Antibiotic sensitivity ; Bacterial cell ; Bactericidal activity ; Interferometry ; Light damage ; Nonhuman ; Optical instrumentation ; Optical tweezers ; Single cell analysis ; Bacteria (microorganisms) ; Escherichia coli
  8. Source: Biomedical Optics Express ; Volume 6, Issue 1 , 2015 , Pages 112-117 ; 21567085 (ISSN)
  9. URL: https://www.osapublishing.org/boe/abstract.cfm?uri=boe-6-1-112